한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제38권5호
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  • Pages.653-656
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  • 2009
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  • 1226-3311(pISSN)
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  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
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참당귀(Angelica gigas Nakai) 중 Decursin 및 Decursinol Angelate의 분리 및 정제

Isolation and Purification of Decursin and Decursinol Angelate in Angelica gigas Nakai

  • 김강민 (인제대학교 식의약생명공학과) ;
  • 정재연 (인제대학교 식의약생명공학과) ;
  • 황성우 (인제대학교 식의약생명공학과) ;
  • 김묘정 (인제대학교 식의약생명공학과) ;
  • 강재선 (경성대학교 약학과)
  • 발행 : 2009.05.29
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초록

생약재로 사용 중인 Angelica gigas Nakai의 decursin 및 decursinol angelate를 60% ethanol로 최종적으로 추출 후 recycling HPLC 및 HPLC/MS에 의해 각각을 분리하였다. 60% ethanol로 $-20^{\circ}C$에서 추출하였을 경우 95% 순도의 decursin 및 decursinol angelate를 얻을 수 있었고 10번 이상의 recycle를 통해서 순수한 decursin 및 decursinol angelate를 분리할 수 있었다. 분리한 decursin 및 decursinol angelate를 HPLC/MS를 통해 분자량을 확인하였을 경우 m/z=329($[M+H]^+$) 및 m/z=351($[M+Na]^+$)과 같은 결과를 얻을 수 있었다. 이 분리 방법을 통해 decursin 및 decursinol angelate를 통한 의약품 및 식품 등에 관한 여러 연구에 있어 두 물질 각각에 대한 약리학적 효과를 도출하는데 기여할 것이며, 물질표준화에 기여할 것으로 사료된다.

This paper is intended as an investigation of the method of extraction and the analysis by high-performance liquid chromatography mass spectroscopy of decursin and decursinol angelate in the dried root of Angelica gigas Nakai. The extracted decursin and decursinol angelate were the purity of >95% using 60% ethanol at $-20^{\circ}C$ for 12 hours by HPLC analysis. Decursin and decursinol angelate were efficiently isolated using recycling HPLC. The purity of isolated decursin and decursinol angelate was identified as 99.97 and 99.40% by HPLC analysis, respectively. The molecular weights of Decursin and decursinol angelate were also identified as m/z=329 ($[M+H]^+$) and m/z=351 ($[M+Na]^+$) by mass spectroscopy.

키워드

참고문헌

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피인용 문헌

  1. Decursin attenuates hepatic fibrogenesis through interrupting TGF-beta-mediated NAD(P)H oxidase activation and Smad signaling in vivo and in vitro vol.108, pp.2, 2014, https://doi.org/10.1016/j.lfs.2014.05.012
  2. The Improvement Effect of Fermented Angelica gigas Nakai Powder Against Orotic Acid-induced Fatty Liver in Rats vol.24, pp.4, 2014, https://doi.org/10.5352/JLS.2014.24.4.411
  3. Physiochemical Characteristics of Extruded Angelica gigas Nakai Depending on the Extrusion Processing Parameter vol.22, pp.5, 2014, https://doi.org/10.7783/KJMCS.2014.22.5.349
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  16. Bacillus polyfermenticus KJS-2와 참당귀 추출물의 triton WR-1339 유발 고지혈증에 대한 예방효과 vol.28, pp.6, 2009, https://doi.org/10.5352/jls.2018.28.6.726
  17. 참당귀 지상부 추출물의 지표성분 decursin의 분석법 개발 및 검증 vol.34, pp.1, 2009, https://doi.org/10.13103/jfhs.2019.34.1.52
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