Molecular & Cellular Toxicology

The Korean Society of Toxicogenomics and Toxicoproteomics (대한독성유전 단백체학회)
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Expression Profile of Inflammatory Genes in Human Airway Epithelial A549 Cells

  • Sohn, Sung-Hwa (BK21 Oriental Medical Science Center, KyungHee University) ;
  • Ko, Eun-Jung (BK21 Oriental Medical Science Center, KyungHee University) ;
  • Kim, Sung-Hoon (Department of Oriental Pathology, College of Oriental Medicine, KyungHee University) ;
  • Kim, Yang-Seok (BK21 Oriental Medical Science Center, KyungHee University) ;
  • Shin, Min-Kyu (BK21 Oriental Medical Science Center, KyungHee University) ;
  • Hong, Moo-Chang (BK21 Oriental Medical Science Center, KyungHee University) ;
  • Bae, Hyun-Su (BK21 Oriental Medical Science Center, KyungHee University)
  • Published : 2009.03.31
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Abstract

This study was conducted to evaluate the inflammation mechanisms of tumor necrosis factor-$\alpha$ (TNF-$\alpha$), interleukin-4 (IL-4), and IL-$1{\beta}$-induced stimulation of A549 human epithelial cells. In the present study, A549 cells were stimulated with TNF-$\alpha$, IL-4 and IL-$1{\beta}$ to induce expression of chemokines and adhesion molecules involved in eosinophil chemotaxis. The effects of TNF-$\alpha$, IL-4 and IL-$1{\beta}$ on gene expression profiles in A549 cells were evaluated by oligonucleotide microarray and Real time RT-PCR. The gene expression profiles for the A549 cells varied depending on the cytokines. Also, the results of the microarray and Real time RT-PCR revealed that inflammatory-related genes were up-regulated in cytokine stimulated A549 cells. Cytokines can affect inflammation in A549 cells. A microarray-based genomic survey is a high-throughput approach that enables evaluation of gene expression in cytokine stimulated cell lines.

Keywords

References

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