The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Gagam-Gongjin-dan Extract Attenuates Immune Responses to Ovalbumin in Balb/c Mice

Balb/c 마우스에서 Ovalbumin 면역반응에 대한 가감공진단(加減拱辰丹) 추출물의 억제효과

  • Kim, Hong-Jun (Department of Oriental Medical Prescription, College of Oriental Medicine, Woosuk University) ;
  • Hwang, Sung-Yeoun (Department of Oriental Medical Prescription, College of Oriental Medicine, Woosuk University) ;
  • Mok, Ji-Ye (Atopy & Health Research Institute, Jeonju University) ;
  • Hwang, Byung-Soon (Jeonju Biomaterials Institute) ;
  • Jeong, Seung-Il (Jeonju Biomaterials Institute) ;
  • Jang, Seon-Il (Atopy & Health Research Institute, Jeonju University)
  • 김홍준 (우석대학교 한의과대학 본초방제학교실) ;
  • 황성연 (우석대학교 한의과대학 본초방제학교실) ;
  • 목지예 (전주대학교 아토피.건강전문연구소) ;
  • 황병순 (전주생물소재연구소) ;
  • 정승일 (전주생물소재연구소) ;
  • 장선일 (전주대학교 아토피.건강전문연구소)
  • Published : 2009.12.30
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Abstract

Objectives : Gagam-Gongjin-dan (GGD) composited with Cervi parvum Cornu, Corni Fructus, Angelica gigantis Radix, Lycii Fructus, Dioscoreae Rhizoma, Citri Pericarpium, Gastrodiae Rihzoma, Agastachis Herba, Cassiae cortkex, Scutellariae Radix, Schisandrae Fructus has been traditionally used for chronic diseases or weakness after illness in oriental countries. However, little is known about the effects of methanol extract of GGD on immune responses to ovalbumin (OVA) plus alum. Therefore, the purpose of this study was to investigate the effects of GGD on immune responses to ovalbumin plus alum in Balb/c mice. Methods : In this study, the extract of GGD was prepared by extracting with methanol for 7 days. The extract was freeze-dried following filtration through vacuum distillation system. Mice were orally administrated with or without GGD extract of different doses (50-200 mg/kg/day) for 30 days. We examined the effects of GGD extract on the serum levels of total IgE, OVA-specific IgE, IgG1, IgG2a, and CTACK/CCL27 production and CCR10 expression in lymph node cells and body weight change and foot pad swelling responses in ovalbumin treated Balb/c. Results : The oral administration of GGD dose-dependently reduced the serum levels of total IgE, OVA-specific immunoglobulin (IgE, IgG1 and IgG2b) and CTACK/CCL27 production in ovalbumin treated BALB/c mice. The levels of CCR10 expression from lymph node cells of OVA treated mice were markedly suppressed by treatment with GGD in a concentration dependent manner. Furthermore, foot pad swelling responses were also markedly suppressed by GGD. However, body weight were significantly increased dose dependently by GGD treatment. Conclusions : These results suggest that GGD treatment suppresses immune responses to ovalbumin, and these properties may contribute to allergic disease care.

Keywords

References

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