The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Anxiolytic and Antidepressive Effect of Non-saponin Fraction of Korean Red Ginseng

홍삼 비사포닌 분획물의 항불안 및 항우울에 대한 효과

  • Lee, Beom-Joon (East-west Medicine Depart. Graduate School of East-west Medical Science, Kyung Hee University) ;
  • Kim, Jung-Woo (East-west Medicine Depart. Graduate School of East-west Medical Science, Kyung Hee University) ;
  • Ji, Eun-Young (East-west Medicine Depart. Graduate School of East-west Medical Science, Kyung Hee University) ;
  • Yun, Seung-Youn (East-west Medicine Depart. Graduate School of East-west Medical Science, Kyung Hee University) ;
  • Lee, Sang-Myung (KT&G Central Research Institute) ;
  • Lew, Jae-Hwan (East-west Medicine Depart. Graduate School of East-west Medical Science, Kyung Hee University)
  • 이범준 (경희대학교 동서의학대학원 동서의학과) ;
  • 김정우 (경희대학교 동서의학대학원 동서의학과) ;
  • 지은영 (경희대학교 동서의학대학원 동서의학과) ;
  • 윤승연 (경희대학교 동서의학대학원 동서의학과) ;
  • 이상명 (KT&G 중앙연구소) ;
  • 류재환 (경희대학교 동서의학대학원 동서의학과)
  • Published : 2009.12.30
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Abstract

Objectives : Anxiety and depression are stress-related disorders. Their prevalence are increasing rapidly. Ginseng is the root of Panax ginseng C.A. Meyer (Araliaceae) which has been used for many centuries in asian region. Anxiolytic effect is one of the popular effects of ginseng. Several studies reported saponin fraction of ginseng, including ginsenoside, is a major ingredient of anxiolytic effect. In present study, we investigated anxiolytic-like and antidepressant-like effect of non-saponin fraction in mice. Material and Method : Mice were divided into five groups. Experimental groups were administered non-saponin fractions (25 mg/kg; nsp25, 50 mg/kg; nsp50, 100 mg/kg; nsp100) respectively once a day in the morning at 9am for 1 week. Then, we performed elevated plus-maze (EPM) test for investigating the anxiolytic-like effect and forced swimming test (FST) for investigating the antidepressant-like action. Results : Non-saponin fraction 50 mg/kg group increased frequency and time spent (p<0.05) in open arm on EPM test and decreased immobility time (p<0.05) on FST compared with control group. Conclusions : We suggest that non-saponin fraction has anxiolytic-like effect and antidepressant like effect in mice.

Keywords

References

  1. Rai D, Bhatia G, Sen T, Palit G. Anti-stress Effects of Ginkgo biloba and Panax ginseng: a Comparative Study. J Pharmacol Sci. 2003 ; 93(4) : 458-64. https://doi.org/10.1254/jphs.93.458
  2. Holmes A, Heilig M, Rupniak NM, Steckler T, Griebel G. Neuropeptide systems as novel therapeutic targets for depression and anxiety disorders. Trends Pharmacol Sci. 2003 ; 24(11) : 580-8. https://doi.org/10.1016/j.tips.2003.09.011
  3. Sutanto W, de Kloet ER. The use of various animal models in the study of stress and stressrelated phenomena. Lab Anim. 1994 ; 28(4) : 293-306 https://doi.org/10.1258/002367794780745092
  4. 이미정, 김은혜, 이동권. 스트레스에 대한 고려 인삼의 효능. 고려인삼학회지 2008 ; 32(1) : 8-14
  5. 백현, 김장현. 감맥대조탕(甘麥大棗湯)이 신체부동 스트레스 백서(白鼠)에 미치는 영향(影響). 대한한의학회지. 2007 ; 28(3) : 183-96
  6. 권보형, 이상용. 구속스트레스 흰쥐에 미치는 사물안신탕 효능에 관한 연구. 동의신경정신과학회지. 1994 ; 5(1) : 81-91
  7. 류종훈, 김민선, 황영선, 육창수. Elevated plus-maze를 이용한 신기환, 보중익기탕 및 사물탕의 항불안효과. 응용약물학회지. 2001 ; 9(2) : 125-30
  8. Luo YM, Cheng XJ, Yuan WX. Effects of ginseng root saponins and ginsenoside Rb1 on immunity in cold water swim stress mice and rats. Zhongguo Yao Li Xue Bao. 1993 ; 14(5) : 401-4
  9. 박정일, 오기완. 항불안효과 및 그 관련연구. 최신고려인삼연구. 2007 ; 105-12
  10. Kim DH, Jung JS, Suh HW, Huh SO, Min SK, Park JH, Kim ND, Kim YH, Song DK. Inhibition of stress-induced plasma corticosterone levels by ginsenosides in mice: involvement of nitric oxide. Neuroreport. 1998 ; 9 : 2261–4. https://doi.org/10.1097/00001756-199807130-00021
  11. Kim DH, Moon YS, Jung JS, Min SK, Son BK, Suh HW, Song DK. Effects of ginseng saponin administered intraperitoneally on the hypothalamopituitary- adrenal axis in mice. Neurosci Lett. 2003 ; 343(1) : 62–6 https://doi.org/10.1016/S0304-3940(03)00300-8
  12. Lee SH, Jung BH, Kim SY, Lee EH. Chung BC. The anti stress effect of ginseng total saponin and ginsenoside Rg3 and Rb1 evaluated by brain polyamine level under immobilization stress. Pharmacol Res. 2006 ; 54 : 46-9 https://doi.org/10.1016/j.phrs.2006.02.001
  13. Kim DH, Moon YS, Lee TH, Jung JS, Suh HW, Song DK. The inhibitory effect of ginseng saponins on the stress-induced plasma interleukin-6 level in mice. Neurosci Lett. 2003 ; 353(1) : 13-6. https://doi.org/10.1016/j.neulet.2003.08.070
  14. Cha HY, Seo JJ, Park JH, Choi KJ, Hong JT, Oh KW. Anxiolytic Effects of Total Saponin Fraction from Ginseng Radix Rubra on the Elevated Plus- Maze Model in Mice. J Ginseng Res. 2004; 28(3): 132-5 https://doi.org/10.5142/JGR.2004.28.3.132
  15. Cha HY, Park JH, Hong JT. Yoo HS, Song SG, Hwang BY, Eun JS, Oh KW. Anxiolytic-Like Effects of Ginsenosides on the Elevated Plus-Maze Model in Mice. Biol Pharm Bull. 2005 ; 28(9) : 1621-5. https://doi.org/10.1248/bpb.28.1621
  16. Kim TW, Choi HJ, Kim NJ, Kim DH. Anxiolyticlike effects of ginsenosides Rg3 and Rh2 from red ginseng in the elevated plus-maze model. Planta Med. 2009 ; 75(8) : 836-9. https://doi.org/10.1055/s-0029-1185402
  17. Lee SH, Jung BH, Kim SY, Lee EH, Chung BC. The antistress effect of ginseng total saponin and ginsenoside Rg3 and Rb1 evaluated by brain polyamine level under immobiliztion stress. Pharmacol Res. 2006 ; 54 : 46-9. https://doi.org/10.1016/j.phrs.2006.02.001
  18. Choi KT. Botanical characteristics, pharmacological effects and medicinal components of Korean Panax ginseng C A Meyer. Acta Pharmacol Sin. 2008 ; 29 (9) : 1109-18. https://doi.org/10.1111/j.1745-7254.2008.00869.x
  19. Okuda H, Lee SD, Matsuura Y, Zheng Y, Sekiya K, Takaku T, Kameda K, Hirose K, Sakata T. Biological Activities of Non-saponin Compounds Isolated from Korean Red Ginseng. 고려인삼학회, International Symposium on Ginseng. 1990 ; 7 : 15-9.
  20. Han BH, Park MH, Han YN, Suh DY. Chemical and Biochemical Studies on Non-saponin constituents of Korean Ginseng. Journal of Ginseng Research. 1992 ; 16(3) : 228-34.
  21. Rodgers RJ, Daly A. Anxiety, Defence and the Elevated plus-maze. Neurosci Biobehav Rev. 1997; 21 (6) : 801-10. https://doi.org/10.1016/S0149-7634(96)00058-9
  22. Petit-Demouliere B, Chenu F. Bourin M. Forced swimming test in mice: a review of antidepressant activity. Psychopharmacology (Berl). 2005 ; 177 : 245-55. https://doi.org/10.1007/s00213-004-2048-7
  23. Lister RG. The use of a plus-maze to measure anxiety in the mouse. Psychopharmacology (Berl). 1987 ; 92 : 180-5.
  24. Porsolt RD, Anton G, Blavet N, Jalfre M. Behavioural despair in rats: a new model sensitive to antidepressant treatments. Eur J Pharmacol. 1978 ; 47 : 379-91. https://doi.org/10.1016/0014-2999(78)90118-8
  25. Zhang ZJ. Therapeutic effects of herbal extracts and constituents in animal models of psychiatric disorders. Life Sci. 2004 ; 75(14) : 1659-99 https://doi.org/10.1016/j.lfs.2004.04.014
  26. Ma Y, Eun JS, Oh KW. Therapeutic Effects of Ginseng on Psychotic Disorders. J Ginseng Res. 2007 ; 31(3) : 117-26 https://doi.org/10.5142/JGR.2007.31.3.117
  27. Hogg S. A review of the validity and variability of the elevated plus-maze as an animal model of anxiety. Pharmacol Biochem Behav. 1996 ; 54(1) : 21-30. https://doi.org/10.1016/0091-3057(95)02126-4
  28. Kasuga S, Ushijima M, Morihara N, Itakura Y, Nakata Y. Effect of aged garlic extract (AGE) on hyperglycemia induced by immobilization stress in mice. Nippon Yakurigaku Zasshi. 1999 ; 114(3) : 191-7. https://doi.org/10.1254/fpj.114.191
  29. Marti O, Martín M, Gavaldà A, Giralt M, Hidalgo J, Hsu BR, Kuhn RW, Armario A. Inhibition of corticosteroid-binding globulin caused by a severe stressor is apparently mediated by the adrenal but not by glucocorticoid receptors. Endocrine. 1997 ; 6(2) : 159-64. https://doi.org/10.1007/BF02738959
  30. Bertram G Katzung. Katzung's 임상약리학. 한우리. 1998 ; 404-23.
  31. 박종대, 고려인삼의 화학성분에 관한 고찰. 고려인삼학회지. 1996 ; 20(4) : 389-415
  32. Pleuvry BJ. CNS stimulants: basic pharmacology and relevance to anaesthesia. Anaesthesia & intensive care medicine. 2006 ; 7(2) : 60-2 https://doi.org/10.1383/anes.2006.7.2.60
  33. Carr MN, Bekku N, Yoshimura H. Identification of anxiolytic ingredients in ginseng root using the elevated plus-maze test in mice. Eur J Pharmacol. 2006 ; 531(1-3) : 160-5 https://doi.org/10.1016/j.ejphar.2005.12.014
  34. Porsolt RD, Le Pichon M, Jalfre M. Depression: a new animal model sensitive to antidepressant treatments. Nature. 1977 ; 266(5604) : 730-2. https://doi.org/10.1038/266730a0
  35. Dhir A, Kulkarni SK. Effect of Addition of Yohimbine (Alpha-2-Receptor Antagonist) to the Antidepressant Activity of Fluoxetine or Venlafaxine in the Mouse Forced Swim Test. Pharmacology. 2007 ; 80(4) : 239-43. https://doi.org/10.1159/000104877