The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
권호 표지
DOI QR코드

DOI QR Code

The Effect of Mibaeksan(MB) on Melanin Synthesis and Gene Expression

미백산(美白散)이 멜라닌 생성 및 유전자 발현에 미치는 영향

  • Kim, Soo-Min (Dept. of Oriental Medicine Graduate School, Daejeon University) ;
  • Yoo, Dong-Youl (Dept. of Oriental Medicine Graduate School, Daejeon University)
  • 김수민 (대전대학교 한의과대학 부인과교실) ;
  • 유동열 (대전대학교 한의과대학 부인과교실)
  • Published : 2009.11.27
Download PDF
⟨ Previous Next ⟩

Abstract

Purpose: This study was performed to elucidate the inhibitory effect of Mibaeksan (MB) on melanin synthesis in B16F10 mouse melanoma cell. Methods: To demonstrate the inhibitory effects of MB on melanin synthesis, we measured the amount of released and produced melanin in B16F10 melanoma cell. Also, we evaluated tyrosinase-activity in vitro as well as in B16F10 melanoma cell. And to investigate the action mechanism, we assessed the gene expression of tyrosinase, TRP-1, TRP-2, MMP-2, PKA, $PKC{\beta}$, ERK-1 ERK-2, AKT-1 and MITF in B16F10 melanoma cells. Results: 1. MB decreased the release and production of melanin in B16F10 melanoma cells. 2. MB decreased tyrosinase activity in vitro and in B16F10 melanoma cells. 3. MB decreased the expression of tyrosinase, TRP-1, TRP-2, PKA, $PKC{\beta}$ and MMP-2 in B16F10 melanoma cells. 4. MB increased the expression of ERK-1, ERK-2 and AKT-1 in B16F10 melanoma cells. 5. MB decreased the expression of MITF in B16F10 melanoma cells. Conclusion: From these results, it may be concluded that MB has the antimelanogenetic effects.

Keywords

References

  1. 김은기, 이향복. 기능성 화장품-미백. NEW & INFORMATION FOR CHEMICAL ENGINEERS. 2007;25(2):138-43.
  2. 2000년 화장품공업협회 생산수입실적 자료.
  3. 한덕희, 박길순. 미백화장품의 구매실태 및 만족도 분석. 한국패션뷰티학회지. 2006;4(4):42-55.
  4. Kameyama K et al. Pigment production in murine melanoma cells is regulated by tyrosinase. TRP-1, TRP-2 and a melanogenic inhibitor. J. Invest Dermatol.. 1993;100:126. https://doi.org/10.1111/1523-1747.ep12462778
  5. J. Cabanes, S. Chazarra, F. Garcia-Carmona. Kojic acid, a cosmetic skin whitening agent, is a slow-blinding inhibitor of catecholase activity of tyrosinase. J. Pharm Pharmacol. 1994;46:982. https://doi.org/10.1111/j.2042-7158.1994.tb03253.x
  6. M. Veronique, B. Friedrich. Tyrosinase and related protein in mammalian pigmenttation. FEBS Letters. 1996;381:165. https://doi.org/10.1016/0014-5793(96)00109-3
  7. 楊維傑 編. 黃帝內經 素門. 臺化: 樂羣出版社業 有限公司. 1994;624-79.
  8. 신연상, 노석선. 기미에 관한 문헌적 고찰. 대한외관과학회지. 1998;11(1):82-98.
  9. 巢元方. 諸病源候論. 北京:人民軍醫出版社. 2006;290.
  10. 王肯堂. 證治準繩. 北京:中國中醫藥出版社. 1997;281.
  11. 송종석, 유동열. 적하수오의 멜라닌 생성 억제와 작용기전에 관한 연구. 대한한방부인과학회지. 2008;21(2):59-75.
  12. 장두현. 시엽의 멜라닌 생성억제와 작용기전에 관한 연구. 대전대 대학원. 2008.
  13. 양현옥 등. 산수유 추출물이 B16F10 melanoma 세포주의 멜라닌 생성에 미치는 영향. 동의병리학회지. 2002;16(4):818-22.
  14. 박지선 등. B16 melanoma 세포주의 멜라닌 합성에 대한 西施玉容散의 효과. 대한동의병리학회지. 2000;14(1):160-70.
  15. 홍의석등. 백지의 $\alpha$-Melanocyte Stimulating Hormone에 유도된 B16 흑색종 세포의 멜라닌 형성에 미치는 영향. 한방안이비인후피부과학회지. 2005;18(1):1-2.
  16. 이관순 등. 天花粉이 멜라닌 형성에 미치는 영향. 대한외관과학회지. 2001;14(1):209-25.
  17. 이종구 등. 황백으로부터 멜라닌 생합성 억제 물질의 분리. 생약학회지. 2007;38(4):387-93.
  18. 대전대학교 한방병원. 한방병원처방집. 대전:한국출판사. 2001:549.
  19. Hosoi J et al. Regulation of melanin synthesis of B16F10 mouse melanoma cells by vitamin D$_{3}$ and retinoic acid. Cancer Res. 1985;45:1474-8.
  20. Mason HS, Peterson EW. Melanoproteins I. Reaction between enzyme-generated quinones and amino acids. Biochim Biophys Acta. 1965;111:134-46. https://doi.org/10.1016/0304-4165(65)90479-4
  21. Martinez EM. Mechanismand melanogenesis inhibition by tumor necrosis factor-a in B16F10 mouse melanoma cells. Eur. J. Biochem. 1998;225:139-46.
  22. 吳克潛. 病源辭典. 서울:萬岳出版社. 1975:458.
  23. 이상희. 麻黃 및 摩風膏의 미백효과에 관한 연구. 경희대학교 동서의학대학원. 2001.
  24. 한방여성의학 편찬위원회. 한방여성의학 상. 서울:정담. 2007:453, 455.
  25. 김창민 등. 중약대사전. 서울:정담. 1999:261, 4015, 4179, 4435, 5053, 5083.
  26. 전국한의과대학본초학교수. 본초학. 서울:영림사. 1995:129, 182, 185, 514, 568, 594.
  27. Abdel MZ et al. Mitogenic and melanogenic stimulating of normal human melanocytes by melanotropic peptides. Proc Natl Acad Sci USA. Feb 28 1995;92(5):1789-93. https://doi.org/10.1073/pnas.92.5.1789
  28. Abdel MZ et al. The melanocortin-1 receptor and humnan pigmentation. Ann NY Acad Sci. Oct 20 1999;885:117-33. https://doi.org/10.1111/j.1749-6632.1999.tb08669.x
  29. 山孝一 良, Hearing VJ. メラニソ産生の 制御因子. Fragnance Journal. 1990;6:24-8.
  30. Kim DS et al. A new 2-imino-1,3-thiazoline derivative. KHG22394, inhibits melanin synthesis in mouse B16 melanoma cells. Biol Pharm Bull. 2007;30(1):180-3. https://doi.org/10.1248/bpb.30.180
  31. VJ Hearing, K Tsukamoto. Biochemical control of melanogenesis and melanosomal organization. J. Invest. Dermatol. 1999;4:24-8. https://doi.org/10.1038/sj.jidsp.5640176
  32. Lee J, Kim YS, Park D. Rosmarinic acid induces melanogenesis through protein kinase A activation signaling. Biochem Pharmacol. 2007;74(7):960-8. https://doi.org/10.1016/j.bcp.2007.06.007
  33. Bertolotto C et al. In B16 melanoma cells, the inhibition of melanogenesis by TPA results from PKC activation and diminution of microphthalmia binding to the M-box of the tyrosinase promoter. Oncogene. 1998;16(13):1665-70. https://doi.org/10.1038/sj.onc.1201685
  34. Huang SC et al. Carnosol inhibits the invasion of B16/F10 mouse melanoma cells by suppressing metalloproteinase-9 through down-regulating nuclear factor -kappa B and c-Jun. Biochem. Pharmacol. 2004;69(2):221-32. https://doi.org/10.1016/j.bcp.2004.09.019
  35. Kim DS et al. Sphingosin-1-phophate decrease melanin synthesis via sustained ERK activation and subsequent MITF degradation. J. Cell Sci. 2003;116:1699. https://doi.org/10.1242/jcs.00366
  36. Jimenez CC et al. Inhibition of melanogenesis in response to oxidative stress: transient downregulation of melanocyte differentiation markers and possible involvement of microphthalmia transcription factor. J. Cell Sci. 2004;114(Pt 12):2335-44.