Effect of Irradiation of Red Radish Seeds on the Seed Viability and Functional Properties of Sprouts

  • Waje, Catherine K. (Department of Food Science and Technology, Kyungpook National University) ;
  • Park, Ju-Hwan (Department of Food Science and Technology, Kyungpook National University) ;
  • Kim, Gui-Ran (Department of Food Science and Technology, Kyungpook National University) ;
  • Kim, Yu-Ri (EB Tech Co., Ltd.) ;
  • Han, Bum-Soo (EB Tech Co., Ltd.) ;
  • Lee, Yeon-Kyung (Department of Food Science and Nutrition, Kyungpook National University) ;
  • Moon, Kwang-Deog (Department of Food Science and Technology, Kyungpook National University) ;
  • Kwon, Joong-Ho (Department of Food Science and Technology, Kyungpook National University)
  • Published : 2009.04.30

Abstract

Red radish seeds were irradiated at doses up to 8 kGy using electron beam (e-beam) and gamma ray ($\gamma$-ray). The seed viability and functional properties (carotenoid, chlorophyll, ascorbic acid, and total phenol) of sprouts grown from these irradiated seeds were evaluated. High germination percentage ($\geq$97%) was observed in seeds irradiated at $\leq$5 kGy, but the yield ratio and sprout length significantly decreased with increased irradiation dose. Irradiation at $\geq$6 kGy resulted in curling of the sprout roots. Sprouting enhanced the functional properties of red radish seeds as indicated by the increased carotenoid, chlorophyll, ascorbic acid, and total phenol contents during germination. However, radiation treatment hampered the growth of seeds resulting in underdeveloped sprouts with decreased carotenoid, chlorophyll, ascorbic acid, and total phenol contents. In general, e-beam and $\gamma$-ray irradiation of red radish seeds showed similar effects on the seed viability and functional properties of sprouts. Postharvest storage reduced the functional quality of sprouts.

Keywords

References

  1. Barillari J, Cervellati R, Paolini, M, Tatibouet A, Rollin P, Iori R. Isolation of 4-methylthio-3-butenyl glucosinolate from Raphanus sativus sprouts (Kaiware daikon) and its redox properties. J. Agr. Food Chem. 53: 9890-9896 (2005) https://doi.org/10.1021/jf051465h
  2. Frias J, Zielinski H, Piskula MK, Kozlowska H, Vidal-Valverde C. Inositol phosphate content and trypsin inhibitor activity in ready-toeat cruciferous sprouts. Food Chem. 93: 331-336 (2005) https://doi.org/10.1016/j.foodchem.2004.09.031
  3. Sikora E, Cieslik E, Leszczynska T, Filipiak-Florkiewicz A, Pisulewski PM. The antioxidant activity of selected cruciferous vegetables subjected to aquathermal processing. Food Chem. 107:55-59 (2008) https://doi.org/10.1016/j.foodchem.2007.07.023
  4. Takaya Y, Kondo Y, Furukawa T, Niwa M. Antioxidant constituents of radish sprouts (Kaiware-daikon), Raphanus sativus L. J. Agr. Food Chem. 51: 8061-8066 (2003) https://doi.org/10.1021/jf0346206
  5. Lee SO, Lee IS. Induction of quinone reductase, the phase 2 anticarcinogenic marker enzyme, in Hepa1c1c7 cells by radish sprouts, Raphanus sativus L. J. Food Sci. 71: S144-S148 (2006) https://doi.org/10.1111/j.1365-2621.2006.tb08917.x
  6. O'Hare TJ, Wong LS, Force LE, Gurung CB, Irving DE, Williams DJ. Glucosinolate composition and anti-cancer potential of daikon and radish sprouts. ISHS Acta Hort. 765: 237-244 (2008)
  7. Waje CK, Kwon JH. Improving the food safety of seed sprouts through irradiation treatment. Food Sci. Biotechnol. 16: 171-176 (2007)
  8. National Advisory Committee on Microbiological Criteria for Foods. Microbiological safety evaluations and recommendations on sprouted seeds. Int. J. Food Microbiol. 52: 123-153 (1999) https://doi.org/10.1016/S0168-1605(99)00135-X
  9. Waje CK, Jun SY, Lee YK, Kim BN, Han DH, Jo C, Kwon JH. Microbial quality assessment and pathogen inactivation by electron beam and gamma irradiation of commercial seed sprouts. Food Control 20: 200-204 (2009) https://doi.org/10.1016/j.foodcont.2008.04.005
  10. Code of Federal Regulations. 21 CFR part 179 irradiation in the production processing and handling of food. US Federal Register 65: 64605-64607 (2000)
  11. Buck JW, Walcott RR, Beuchat LR. Recent trends in microbiological safety of fruits and vegetables. Plant Health Progress. Available from: http://www.plantmanagementnetwork.org. Accessed May, 2008
  12. Kim HJ, Feng H, Kushad MM, Fan X. Effects of ultrasound, irradiation, and acidic electrolyzed water on germination of alfalfa and broccoli seeds and Escherichia coli O157:H7. J. Food Sci. 71:168-173 (2006) https://doi.org/10.1111/j.1750-3841.2006.00064.x
  13. Rajkowski KT, Boyd G, Thayer DW. Irradiation D-values for Escherichia coli O157:H7 and Salmonella sp. on inoculated broccoli seeds and effects of irradiation on broccoli sprout keeping quality and seed viability. J. Food Protect. 66: 760-790 (2003) https://doi.org/10.4315/0362-028X-66.5.760
  14. Association of Official Seed Analysts. Rules for Testing Seeds. Graphic Publishing Company, Inc., Boston, MA, USA. pp. 26-32 (1965)
  15. Fan X, Thayer DW. Quality of irradiated alfalfa sprouts. J. Food Protect. 64: 1574-1578 (2001) https://doi.org/10.4315/0362-028X-64.10.1574
  16. Lichtenthaler HK, Wellburn AR. Determination of total carotenoids and chlorophylls a and b of leaf in different solvents. Biochem. Soc. T. 11: 591-592 (1985)
  17. AOAC. AOAC Official Methods of Analysis. 17th ed. Method 967.21. Association of Official Analytical Chemists, Arlington, VA, USA (2000)
  18. Schanderl SH. Methods in Food Analysis. 2nd ed. Academic Press, London, Elgland. pp. 701-725 (1970)
  19. Rajkowski KT, Thayer DW. Alfalfa seed germination and yield ratio and alfalfa sprout microbial keeping quality following irradiation of seeds and sprouts. J. Food Protect. 64: 1988-1995 (2001) https://doi.org/10.4315/0362-028X-64.12.1988
  20. Fan X, Thayer DW, Sokorai K. Changes in growth and antioxidant status of alfalfa sprouts during sprouting as affected by gamma irradiation of seeds. J. Food Protect. 67: 561-566 (2004) https://doi.org/10.4315/0362-028X-67.3.561
  21. Randhir R, Lin YT, Shetty K. Phenolics, their antioxidant and antimicrobial activity in dark germinated fenugreek sprouts in response to peptide and phytochemical elicitors. Asia Pac. J. Clin. Nutr. 13: 295-307 (2004)
  22. Vidal-Valverde C, Frias J, Sierra I, Blazquez I, Lambein F, Kuo YH. New functional legume food by germination: Effect on the nutritive value of beans, lentils, and peas. Eur. Food Res. Technol. 215: 472-477 (2002) https://doi.org/10.1007/s00217-002-0602-2
  23. Urbano G, Lopez-Jurado M, Hernandez J, Fernandez M, Moren MC, Frias J, Diaz-Pollan C, Prodanov M, Vidal-Valverde C. Nutritional assessment of raw, heated, and germinated lentils. J. Agr. Food Chem. 43: 1871-1877 (1995) https://doi.org/10.1021/jf00055a022
  24. Khattak AB, Zeb A, Bibi N. Impact of germination time and type of illumination on carotenoid content, protein solubility, and in vitro protein digestibility of chickpea (Cicer arietinum L.) sprouts. Food Chem. 109: 797-801 (2008) https://doi.org/10.1016/j.foodchem.2008.01.046
  25. Fernandez-Orozco R, Piskula MK, Zielinski H, Kozlowska H, Frias J, Vidal-Valverde C. Germination as a process to improve the antioxidant capacity of Lupinus angustifolius L. var. Zapaton. Eur. Food Res. Technol. 223: 495-502 (2006) https://doi.org/10.1007/s00217-005-0229-1
  26. Fan X, Rajkowski KT, Thayer DW. Quality of alfalfa sprouts grown from irradiated seeds. J. Food Quality 26: 165-176 (2003) https://doi.org/10.1111/j.1745-4557.2003.tb00235.x
  27. Frias J, Miranda ML, Doblado R, Vidal-Valverde C. Effect of germination and fermentation on the antioxidant vitamin content and antioxidant capacity of Lupinus albus L. var. Multolupa. Food Chem. 92: 211-220 (2005) https://doi.org/10.1016/j.foodchem.2004.06.049
  28. Lopez-Amoros ML, Hernandez T, Estrella I. Effect of germination on legume phenolic compounds and their antioxidant activity. J. Food Compos. Anal. 19: 277-283 (2006) https://doi.org/10.1016/j.jfca.2004.06.012
  29. MacCue P, Shetty K. Clonal herbal extracts as elicitors of phenolic synthesis in dark germinated mungbean for improving nutritional value with implications for food safety. J. Food Biochem. 26: 209-232 (2002) https://doi.org/10.1111/j.1745-4514.2002.tb00853.x
  30. Bari ML, Al-Haq MI, Kawasaki T, Nakauma M, Todoriki S, Kawamoto S, Isshiki K. Irradiation to kill Escherichia coli O157:H7 and Salmonella on ready-to eat radish and mungbean sprouts. J. Food Protect. 67: 2263-2268 (2004) https://doi.org/10.4315/0362-028X-67.10.2263
  31. Rajkowski KT, Thayer DW. Reduction of Salmonella spp. and strains of Escherichia coli O157:H7 by gamma irradiation of inoculated sprouts. J. Food Protect. 63: 871-875 (2000) https://doi.org/10.4315/0362-028X-63.7.871
  32. Thayer DW, Boyd G, Fett WF. Gamma irradiation decontamination of alfalfa seeds naturally contaminated with Salmonella mbandaka. J. Food Sci. 68: 2266-2270 (2002)
  33. Schoeller NP, Ingham SC, Ingham BH. Assessment of the potential for Listeria monocytogenes survival and growth during alfalfa sprout production and use of ionizing radiation as a potential intervention treatment. J. Food Protect. 65: 1259-1266 (2002) https://doi.org/10.4315/0362-028X-65.8.1259