Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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In vitro selection of lactic acid bacteria for probiotic use in pig

양돈용 생균제 균주개발을 위한 유산균주 선발

  • Ryu, Ji-Sook (Gyeonggido Veterinary Service) ;
  • Han, Sun-Kyung (Department of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University) ;
  • Shin, Myeong-Soo (Department of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University) ;
  • Lee, Wan-Kyu (Department of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University)
  • Published : 2009.03.30
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Abstract

In order to develop probiotic strain for pigs, Lactobacillus spp. (527 isolates), Streptococcus spp. (95 isolates) and Bifidobacterium spp. (25 isolates) were isolated from the feces of 35 pigs. These isolates were tested through in vitro experiment such as acid tolerance at pH 2.0 (Lactobacillus spp. and Streptococcus spp.) or pH 3.0 (Bifidobacterium spp.), bile tolerance in MRS broth containing 0.3% (w/v) Oxgall, heat resistance at $70^{\circ}C$ and $80^{\circ}C$ for 5 min, antibiotic resistance, antimicrobial activity against pathogenic bacteria and Caco-2 cell adherence assay. Finally ten most superior strain (5 Lactobacillus spp. strain, 3 Bifidobacterium spp. strain and 2 Streptococcus spp. strain) were selected as potential candidate for probiotic use in pig industry. It could be used as an alternative to antibiotics in feed additives.

Keywords

References

  1. Byun JW, Kim GT, Bae HS, et al. 2000. In vitro selection of lactic acid bacteria for probiotics use in pigs. J Vet Sci 40(4): 701-706
  2. Carl E, Kotarski S. 1999. Evaluation of veterinary drug residues in food for their potential to affect human intestinal microflora. Regulaton Toxicol Pharmacol 29: 238-261 https://doi.org/10.1006/rtph.1999.1300
  3. Chang YH, Kim JK, Kim HJ, et al. 1999. Characteristics of Lactobacillus reuteri BAS-131 Isolated from Swine Intεstine. Kor J Microbiol Biotechnol 27(1): 23-27
  4. Collins JK, Thornton G, Sullivan GO. 1998. Selection of probiotic strains for human application. Int Dain 18 487-490
  5. Cromwell GL. 2002. Why and how antibiotics are used in swine production. Anim BiotechnoI 13(1): 7-27 https://doi.org/10.1081/ABIO-120005767
  6. Fleming HP, Etchells JL, Costilow RL. 1985. Microbial inhibition by an isolatε of Pediococcus from cucumber brines. Appl Environ Microbiol 30(6): 1040-1042
  7. Fuller R. 1989. Probiotics in man and animals. J Appl BacterioI 66(5): 365-378 https://doi.org/10.1111/j.1365-2672.1989.tb05105.x
  8. Fuller R. 1992. Probiotics: The scientific basis. Chapman & Hall: 259-272
  9. Hilton M, Kaukas A, Linton AH. 1986. The ecology of drug resistance in εnteric bacteria. Soc Appl Bacteriol Symp Ser 15: 77S-92S
  10. Isolauri E, Kirkavainen PV, Salminen S. 2002. Probiotics: a role in the trεatment of intestinal infection and inflam mation. Gut 3: 54-59
  11. Kimoto H, Kurisaki J, Tsuji NM, et al. 1999. Lactococci as probiotic strains: adhesion to human enterocyte-like Caco-2 cells and tolerance to low pH and bile. Letters Appl Microbio 129(5): 313-316
  12. Lee KW, Heo TR. 1999. Isolation and sεlection of beneficial Bifidobacterium spp. Korean J. Food Sci Ani Resour 19(1): 41-53
  13. Lee YK, Salminεn S. 1995. The coming of age of probiotics. Trends Food Sci Technol 6: 241-245 https://doi.org/10.1016/S0924-2244(00)89085-8
  14. Lim HJ, Kim SY, Lee WK. 2004. Isolation and identification of cholesterol - Lowering lactic acid bacteria from human intestinε for probiotic use. J Vet Sci 5(4): 391-395
  15. Mitsuoka T. 1978. Intestinal Bacteria and Health. Harcourt Brace Jovenich, Japan: 86-111
  16. Mitsuoka T. 1980. The world of anaerobic bacteria: A color atlas of anaerobic bacteria. Sobun Press, Tokyo: 13-65
  17. Rolfe RD. 2000. The role of probiotic cultures in the control of gastrointεstinal health. J Nutr 130: 396-402
  18. Salminen S, Ouwehand A, Benno Y, et al. 1999. Probiotics: how should they be defined. Trends Food Sci Technol 10(3): 107-110 https://doi.org/10.1016/S0924-2244(99)00027-8
  19. Zimmerman DR, Conway DP, Bliss DH, et al.. 1982. Effect of carbadox and pyrantel tartrate on performance and indices of Mycoplasma hyopneumoniae and Ascaris suum infection in pig. J Anim Sci 55: 733-740