Phenolic Content, Antioxidant Effect and Acetylcholinesterase Inhibitory Activity of Korean Commercial Green, Puer, Oolong, and Black Teas

국내 시판 녹차, 보이차, 우롱차 및 홍차의 폴리페놀 함량, 항산화 및 아세틸콜린에스터레이스 저해 효과

  • Jeong, Chang-Ho (Division of Applied Life Sciences, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kang, Su-Tae (Department of Food Science and Biotechnology, Pukyong National University) ;
  • Joo, Ok-Soo (Department of Food Science and Technology, Jinju National University) ;
  • Lee, Seung-Cheol (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Shin, Young-Hee (Division of Applied Life Sciences, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Shim, Ki-Hwan (Division of Applied Life Sciences, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Cho, Sung-Hwan (Division of Applied Life Sciences, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Choi, Sung-Gil (Division of Applied Life Sciences, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Heo, Ho-Jin (Division of Applied Life Sciences, Institute of Agriculture and Life Science, Gyeongsang National University)
  • 정창호 (경상대학교 대학원 응용생명과학부.농업생명과학연구원) ;
  • 강수태 (부경대학교 식품생명공학부) ;
  • 주옥수 (진주산업대학교 식품과학과) ;
  • 이승철 (경남대학교 식품생명학과) ;
  • 신영희 (경상대학교 대학원 응용생명과학부.농업생명과학연구원) ;
  • 심기환 (경상대학교 대학원 응용생명과학부.농업생명과학연구원) ;
  • 조성환 (경상대학교 대학원 응용생명과학부.농업생명과학연구원) ;
  • 최성길 (경상대학교 대학원 응용생명과학부.농업생명과학연구원) ;
  • 허호진 (경상대학교 대학원 응용생명과학부.농업생명과학연구원)
  • Published : 2009.04.30

Abstract

The phenolic contents, antioxidant effects, and acetylcholinesterase inhibitory activities of hot water extracts prepared from various Korean commercial teas(green tea, puer tea, oolong tea, and black tea) were investigated. Total phenolic contents were in the range 72.03-85.62 mg/g. Flavonol contents of hot water extracts from green tea, puer tea, oolong tea, and black tea were 350.96, 254.17, 334.48, and 240.23 mg/100 g, respectively. Catechin contents were 2,920.35 mg/100 g in green tea, 1,016.23 mg/100 g in puer tea, 2,824.22 mg/100 g in oolong tea, and 1,006.51 mg/100 g in black tea. The highest caffeine content was in the green tea extract. All four extracts scavenged $ABTS^{{\cdot}+}$ radicals in a concentration-dependent manner, and the green tea extract was the most potent in this regard. The highest reducing power was observed in the green tea extract. All four extracts exhibited considerable antioxidative activities in linoleic acid autoxidation, $\beta$-carotene bleaching, and acetylcholinesterase inhibition assays; the effects were concentration-dependent and decreased in the order green tea > oolong tea > puer tea > black tea.

본 연구에서는 국내에서 시판되고 있는 4종류의 차 즉, 녹차, 보이차, 우롱차 및 홍차 열수추출물의 페놀성 화합물, 항산화 및 퇴행성 뇌신경질환 저해활성에 대하여 조사하였다. 시판 차 열수추출물의 총 페놀성 화합물 함량은 $72.03{\sim}85.62\;mg/g$이었으며, 녹차, 보이차, 우롱차 및 홍차 열수추출물의 총 플라보놀 함량은 각각 350.96, 254.17, 334.48 및 240.23 mg/100 g이었다. 녹차, 보이차, 우롱차 및 홍차 열수추출물의 총 카테킨 함량은 각각 2,920.35, 1,016.23, 2,824.22 및 1,006.51 mg/100 g이었으며, 카페인함량은 녹차 열수추출물에서 가장 높은 함량을 보였다. $ABTS{\cdot}+$ 라디칼 소거활성도 농도 의존적인 경향을 보였으며, FRAP은 다른 차 열수추출물에 비하여 녹차 열수추출물에서 가장 높게 나타났다. $\beta$-carotene과 linoleic acid system을 이용하여 측정한 항산화 활성 및 퇴행성 뇌신경질환효소 억제활성도 녹차 열수추출물에서 가장 높은 효과를 보였다. 따라서 시판 차 4종의 열수추출물을 이용하여 항산화 및 퇴행성 뇌신경질환효소 억제활성을 측정한 결과 녹차 열수추출물이 가장 높은 활성을 보였으며, 홍차 열수추출물이 가장 낮게 나타났다.

Keywords

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