Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
권호 표지

Characteristics of Microorganisms Contaminating Seafood Cooking Drips Exposed to Gamma Irradiation

감마선 조사된 수산 자숙액의 오염 미생물군 특성

  • Choi, Jong-Il (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Yeon-Joo (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Jae-Hun (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Chun, Byung-Soo (Food Science and Biotechnology, Pukyong National University) ;
  • Ahn, Dong-Hyun (Food Science and Biotechnology, Pukyong National University) ;
  • Kwon, Joong-Ho (Department of Food Science & Technology, Kyungpook National University) ;
  • Hwang, Young-Jung (Division of Food Science, Jinju International University) ;
  • Byun, Myung-Woo (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Lee, Ju-Woon (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • 최종일 (한국원자력연구원 정읍방사선과학연구소) ;
  • 김연주 (한국원자력연구원 정읍방사선과학연구소) ;
  • 김재훈 (한국원자력연구원 정읍방사선과학연구소) ;
  • 전병수 (부경대학교 식품생명공학부) ;
  • 안동현 (부경대학교 식품생명공학부) ;
  • 권중호 (경북대학교 식품공학과) ;
  • 황영정 (진주국제대학교 식품과학부) ;
  • 변명우 (한국원자력연구원 정읍방사선과학연구소) ;
  • 이주운 (한국원자력연구원 정읍방사선과학연구소)
  • Published : 2009.04.30
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Abstract

Microorganisms in seafood cooking drips were counted and identified. Total viable cell counts were 6.40 and 3.10 log CFU/g in cooking drips of Hizikia fusiformis and Thunnus thynnus, respectively. However, microbial populations fell with increased irradiation doses. In H. fusiformis cooking drips, a 5-log reduction in total aerobic bacteria was obtained by irradiation with 5 kGy. In T. thynnus cooking drips, however, contaminating microorganisms were more resistant to gamma irradiation and only a 1-log reduction was seen. DNA sequence analysis showed that the principal contaminating microorganisms in H. fusiformis and T. thynnus cooking drips were Lactobacillus and Bacillus species, respectively. Therefore, the high irradiation resistance of T. thynnus cooking drips microbes may result from spore formation by Bacillus species.

수산 가공 부산물인 자숙액을 식품 및 공중보건 소재로 이용하기 위해 자숙액의 위생화를 위한 감마선 조사기술의 이용가능성을 검토하였다. 톳, 참치 및 굴 자숙액을 시료로 하여 1-5 kGy의 감마선을 조사하여 미생물 생존율을 측정하였고 생존미생물을 동정하였다. 굴 자숙액에서는 호기성 미생물이 검출되지 않았으나, 톳 및 참치 자숙액에서는 약 6.4 및 3.1 log CFU/g의 호기성 미생물이 검출되었고, 저장기간이 증가함에 따른 미생물 수에는 유의적인 차이가 없었다. 또한 감마선 조사선량이 증가함에 따라 생균수의 감소와 자숙액에 오염된 미생물의 살균효과를 확인하였으나, 자숙액 종류별로 미생물의 감마선 저항성이 상이하였다. 수산 자숙액에 존재하는 모든 미생물을 사멸시키기 위해서는 톳 자숙액의 경우 10 kGy, 참치자숙액의 경우 20 kGy 이상의 감마선 조사가 필요하다고 생각된다. 자숙액에 오염된 미생물을 동정한 결과 L. plantarum, L. pentosus, Paenibacillus sp., B. subtilis, B. gelatini 등을 포함하여 총 20종의 오염 미생물을 확인할 수 있었다. 톳 자숙액에서는 주로 Lactobacillus 속 들이 주로 검출되었고, 참치 자숙액에서는 Bacillus 속이 주로 검출되었다.

Keywords

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