Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antioxidant and Anticancer Activities of Defatted Soybean Grits Fermented by Bacillus subtilis NUC1

고초균에 의한 탈지대두 Grits 발효물의 항산화 및 항암 활성

  • Lee, Sung-Gyu (Dept. of Food and Technology, Keimyung University) ;
  • Kim, Hyun-Jeong (The Center for Traditional Microorganism Resources, Keimyung University) ;
  • Lee, Sam-Pin (Dept. of Food and Technology, Keimyung University) ;
  • Lee, In-Seon (Dept. of Food and Technology, Keimyung University)
  • 이성규 (계명대학교 식품가공학과) ;
  • 김현정 (계명대학교 전통 미생물자원 개발 및 산업화 연구센터) ;
  • 이삼빈 (계명대학교 식품가공학과) ;
  • 이인선 (계명대학교 식품가공학과)
  • Published : 2009.06.30
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Abstract

Antioxidant and anticancer activities of water and ethanol extracts of defatted soybean grits (DSG) fermented Bacillus subtilis NUC1 were determined and compared with those of the raw DSG. The fermented defatted soybean grits (FDSG) exhibited higher total polyphenols and flavonoids contents than DSG. The ethanol extracts of FDSG (FD-E) showed the highest polyphenol and flavonoid contents with 23.35 mg/g and 3.48 mg/g, respectively. Particularly, FD-E showed higher DPPH radical scavenging activities with $RC_{50}$ of 0.32 mg/mL than other samples with $RC_{50}$ of 1.10${\sim}$3.89 mg/mL. The water and ethanol extracts of FDSG and DSG showed growth inhibitory effects against AGS, A549 and Hela cancer cells in a dose-dependent manner, and especially FD-E showed the highest growth inhibition effects. FD-E induced apoptosis in Hela cells through an increased activation of caspase-3 and caspase-3 target protein, PARP, but rarely affected caspase-7.

본 연구에서는 탈지대두 grits(DSG)를 사용하여 B. subtilis NUC1균주로 발효시켜 DSG 발효물을 제조한 다음 물혹은 에탄올로 추출하여 이들 DSG 발효 추출물의 항산화 및 항암 활성을 살펴보았으며, 이때 발효하지 않은 DSG와 비교 분석하였다. 그 결과, DSG 발효물의 총 폴리페놀 및 총 플라보노이드 함량은 DSG보다 더 증가되었다. 특히 DSG 발효물의 에탄올 추출물(FD-E)에서 총 폴리페놀 및 총 플라보노이드 함량이 각각 23.35 mg/g, 3.48 mg/g으로 가장 높게 나타났다. 또한 DSG 발효물의 에탄올 추출물은 가장 우수한 DPPH 소거활성을 보였으며, $RC_{50}$값도 0.32 mg/mL이었다. 인간유래 암세포주인 AGS, A-549, Hela에 대해 DSG 및 DSG 발효 추출물의 처리 농도가 증가할수록 농도 의존적으로 성장 저해효과가 증가하였고, DSG보다 DSG 발효물에서 더 큰 성장저해 효과를 보였다. 특히 Hela 세포에서 가장 우수한 성장저해 효과를 보인 DSG 발효물 에탄올 추출물은 1.25 mg/mL의 농도에서부터 caspase-3과 PARP 단백질들의 발현을 유도하여 apoptosis가 일어남을 확인하였다. 따라서 DSG 발효물은 항산화 및 항암 활성을 가지는 우수한 천연소재가 되리라 기대된다.

Keywords

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