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α-Amylase Activity of Radish and Stability in Processing

무의 α-Amylase 활성 및 가공 안정성

  • Cho, Eun-Hye (Dept. of Food and Biotechnology, and Center for Food Function and Safety, Hoseo University) ;
  • Choi, A-Reum (Dept. of Food and Biotechnology, and Center for Food Function and Safety, Hoseo University) ;
  • Choi, Sun-Ju (Dept. of Food and Biotechnology, and Center for Food Function and Safety, Hoseo University) ;
  • Kim, So-Young (Dept. of Food and Biotechnology, and Center for Food Function and Safety, Hoseo University) ;
  • Lee, Gun-Soon (Dept. of Living Sciences, Korea National Agriculture College) ;
  • Lee, Soo-Seoug (BioBreeding Institute) ;
  • Chae, Hee-Jeong (Dept. of Food and Biotechnology, and Center for Food Function and Safety, Hoseo University)
  • 조은혜 (호서대학교 식품생물공학과 및 식품기능안전연구센터) ;
  • 최아름 (호서대학교 식품생물공학과 및 식품기능안전연구센터) ;
  • 최선주 (호서대학교 식품생물공학과 및 식품기능안전연구센터) ;
  • 김소영 (호서대학교 식품생물공학과 및 식품기능안전연구센터) ;
  • 이건순 (한국농업대학 교양공통학부) ;
  • 이수성 ((주)바이오브리딩연구소) ;
  • 채희정 (호서대학교 식품생물공학과 및 식품기능안전연구센터)
  • Published : 2009.06.30

Abstract

The effects of processing conditions on the ${\alpha}$-amylase activity of radish were investigated at various temperatures, pHs and drying conditions. The ${\alpha}$-amylase activity of radish root was 3.1-fold higher than that of radish trunk. As the freeze-dried radish was incubated at various temperatures and pHs, ${\alpha}$-amylase activity was stably maintained at pH range of $4{\sim}7$ and temperature of $25{\sim}40^{\circ}C$. When radish was processed to kakdugi and danmooji, the residual ${\alpha}$-amylase activity was 45.39% and 19.19%, respectively. Consequently, the ${\alpha}$-amylase activity was greatly affected by processing conditions such as heat treatment and pH. It is suggested that radish should be processed at below $60^{\circ}C$ and at neutral to acidic pH condition.

소화 촉진 작용이 있다고 알려진 무의 건조방법, 온도, pH 등의 가공조건이 ${\alpha}$-amylase 활성에 미치는 영향을 검토하였다. 동결건조 한 무 뿌리와 무 줄기의 ${\alpha}$-amylase의 활성을 비교한 결과 무 뿌리가 무 줄기의 3.1배 높은 수준의 활성을 보였다. 무를 pH와 온도를 달리하여 안정성을 측정한 결과, pH 범위가 $4{\sim}7$이며 온도가 $25{\sim}40^{\circ}C$일 때 ${\alpha}$-amylase 활성이 높았다. 무의 ${\alpha}$-amylase는 산성이나 중성 조건에서 처리하고 $60^{\circ}C$ 이하의 온도로 가열처리하여야 활성을 유지하였다. 무를 깍두기와 단무지의 형태로 가공하였을 때 무의 ${\alpha}$-amylase 잔류활성은 각각 15.39%와 19.193%이었고 초절임무에서는 ${\alpha}$-amylase의 활성이 대부분 소실됨을 보였다. 결과적으로, 무의 소화효소인 ${\alpha}$-amylase 활성은 열과 pH등의 가공조건에 의해 크게 영향을 받는 것을 알 수 있었으며 $60^{\circ}C$ 이하의 온도에서 열처리하는 것과 중성이나 약산성에서 ${\alpha}$-amylase 효소의 활성도가 유지되는 것으로 판단된다.

Keywords

References

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