Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Reevaluation of Enumeration of Bacillus cereus Grown on Mannitol-Egg York-Polymyxin B Agar

Mannitol-Egg York-Polymyxin B 선택 배지에서 Bacillus cereus 계수 방법의 재평가

  • Yun, Suk-Hyun (Faculty of Biological Sciences and Institute for Molecular Biology and Genetics, Chonbuk National University) ;
  • Kim, Yong-Sang (Faculty of Biological Sciences and Institute for Molecular Biology and Genetics, Chonbuk National University) ;
  • Jeong, Do-Yeon (Institute of Sunchang Fermented Soybean Products) ;
  • Hahn, Kum-Su (Institute of Sunchang Fermented Soybean Products) ;
  • Uhm, Tai-Boong (Faculty of Biological Sciences and Institute for Molecular Biology and Genetics, Chonbuk National University)
  • 윤숙현 (전북대학교 자연과학대학 생물과학부 및 전북대학교 유전공학연구소) ;
  • 김용상 (전북대학교 자연과학대학 생물과학부 및 전북대학교 유전공학연구소) ;
  • 정도연 (순창장류연구소) ;
  • 한금수 (순창장류연구소) ;
  • 엄태붕 (전북대학교 자연과학대학 생물과학부 및 전북대학교 유전공학연구소)
  • Received : 2009.06.05
  • Accepted : 2009.06.22
  • Published : 2009.06.30
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Abstract

To avoid ambiguity in counting the number of colony, about 1,500 of colonies grown on B. cereus selective agar plates were grouped into 12 types by morphological difference and then identified by biochemical and 16S rDNA nucleotide sequence. Among them, seven colony types with 11 to 15 mm diameters of halo were identified as B. cereus or B. cereus subsp. cytotoxis. Five mm sized colonies with no halo, which have not been considered as B. cereus according to the manufacturer's manual, were identified as B. cereus. A colony type with double halos of only 6 mm in diameter was also B. cereus. Other three types were proven to be Enterococcus sp., Brevibacillus sp., and B. subtilis, respectively. PCR results showed that only 9 types that are identified as B. cereus strains harbor at least one of B. cereus toxin genes.

장류에서 식중독 미생물인 Bacillus cereus의 계수 방법상 문제점을 해결하기 위하여, B. cereus 선택 배지인 mannitol-egg york-polymyxin B (MYP)에서 자란 약 1,500개의 집락들을 형태적 차이에 따라 12종으로 분류하였다. 생화학 특성 및 16S rRNA 유전자의 염기서열 분석 결과 집락 주위로 환을 형성하는 직경 11~16 mm 크기의 집락들은 B. cereus 와 B. cereus subsp. cytotoxis로 동정되었고, 환을 형성하지 않아 B. cereus 계수에 포함시키지 않았던 직경 5 mm 크기의 분홍색 집락은 B. cereus로, 6.5 mm 크기의 이중환을 형성하여 B. cereus로 계수하기가 모호하였던 붉은색 집락은 B. cereus로 동정되었다. 나머지 세 종들은 Enterococcus sp., Brevibacillus sp., B. subtilis로 밝혀졌다. B. cereus로 동정된 9종 모두에서 적어도 한 종류 이상의 B. cereus 독소 유전자들을 함유하고 있었지만 다른 형태들에서는 발견되지 않았다.

Keywords

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