Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Functional Analysis of MCNA, a Gene Encoding a Catalytic Subunit of Calcineurin, in the Rice Blast Fungus Magnaporthe oryzae

  • Choi, Jin-Hee (Department of Agricultural Biotechnology, Center for Agricultural Biomaterials, and Center for Fungal Genetic Resources, Seoul National University) ;
  • Kim, Yang-Seon (Department of Agricultural Biotechnology, Center for Agricultural Biomaterials, and Center for Fungal Genetic Resources, Seoul National University) ;
  • Lee, Yong-Hwan (Department of Agricultural Biotechnology, Center for Agricultural Biomaterials, and Center for Fungal Genetic Resources, Seoul National University)
  • Received : 2008.04.10
  • Accepted : 2008.07.01
  • Published : 2009.01.31
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Abstract

Magnaporthe oryzae, the causal agent of rice blast, forms a specialized infection structure, called an appressorium, which is crucial for penetration and infection of the host plant. Pharmacological data suggest that calcium/calmodulindependent signaling is involved in appressorium formation in this fungus. To understand the role of the calcium/calmodulin-activated protein phosphatase on appressorium formation at the molecular level, MCNA, a gene encoding the catalytic subunit of calcineurin, was functionally characterized in M. oryzae. Transformants expressing sense/antisense RNA of MCNA exhibited significant reductions in mycelial growth, conidiation, appressorium formation, and pathogenicity. cDNA of MCNA functionally complemented a calcineurin disruptant strain (cmp1::LEU2 cmp2::HIS3) of Saccharomyces cerevisiae. These data suggest that calcineurin A plays important roles in signal transduction pathways involved in the infection-related morphogenesis and pathogenicity of M. oryzae.

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References

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