대한환경공학회지 (Journal of Korean Society of Environmental Engineers)

  • 제31권2호
  • /
  • Pages.139-146
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  • 2009
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  • 1225-5025(pISSN)
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  • 2383-7810(eISSN)
대한환경공학회 (Korean Society of Environmental Engineers)
권호 표지

두부 폐수를 이용한 수소생산 및 미생물의 군집 변화

Change of Microbial Community and Fermentative Production of Hydrogen from Tofu Wastewater

  • 전윤선 (서울산업대학교 환경공학과) ;
  • 조윤아 (서울산업대학교 환경공학과) ;
  • 이태진 (서울산업대학교 환경공학과)
  • Jun, Yoon-Sun (Department of Environmental Engineering, Seoul National University of Technology) ;
  • Joe, Yoon-A (Department of Environmental Engineering, Seoul National University of Technology) ;
  • Lee, Tae-Jin (Department of Environmental Engineering, Seoul National University of Technology)
  • 투고 : 2008.10.10
  • 심사 : 2009.02.27
  • 발행 : 2009.02.28
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초록

본 연구에서는 두부공장에서 발생된 폐수를 이용한 생물학적 수소생성 특성과 미생물의 군집 변화를 살펴보았다. 두부 폐수는 산 또는 알카리 조건에서 전처리 된 후 수소생성량을 비교 하였으며, 산처리와 열처리를 병행하여 전처리 하였을 때 가장 왕성한 수소생산을 보였으며, Gompartz 방정식을 이용한 수소가스 발생량($P_h$)은 약 661.01 mL이고 최대 수소 생성율($R_h$)은 12.21 mL/g dry wt biomass/hr 이였다. 16S rDNA의 PCR-DGGE 결과 대부분 군집은 Streptococcus sp. 미생물로 규명되었으며 수소생성에 기여도가 큰 미생물은 Streptococcus gallolyticus sub sp.으로 판단되었다.

In this study, characteristics of biological hydrogen production and microbial distribution were investigated with the wastewater of Tofu manufacturing process. Comparison of hydrogen production was conducted with acid or base pre-treatment of the wastewater. Maximum hydrogen production was acquired with combination of heat and acid treatment. Hydrogen production ($P_h$) and maximum hydrogen production rate ($R_h$) was calculated 661.01 mL and 12.21 mL/g dry wt biomass/hr from the modified Gompartz equation. Most of microbial community was analyzed as Streptococcus sp. from PCR-DGGE experiment of 16S rDNA. It was concluded that most significant microorganism for hydrogen production was Streptococcus gallolyticus sub sp. in this experiment.

키워드

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