Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
권호 표지

The Efficacy Evaluation of Tourmaline-Ionized Water in Animal Study

투어마린이온활성수의 효능 평가

  • Yoon, Yang-Suk (Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University) ;
  • Kim, Dong-Heui (Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University) ;
  • Qi, Xu-Feng (Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University) ;
  • Song, Soon-Bong (Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University) ;
  • Jung, Jong-Ho (Chonsubio Co., Ltd.) ;
  • Joo, Kyung-Bok (Department of Ophthalmic Optics, Chodang University) ;
  • Teng, Yung-Chien (Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University) ;
  • Lee, Kyu-Jae (Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University)
  • 윤양숙 (연세대학교 원주의과대학 환경의생물학교실) ;
  • 김동희 (연세대학교 원주의과대학 환경의생물학교실) ;
  • 최주봉 (연세대학교 원주의과대학 환경의생물학교실) ;
  • 송순봉 (연세대학교 원주의과대학 환경의생물학교실) ;
  • 정종효 ((주)천수바이오) ;
  • 주경복 (초당대학교 안경광학과) ;
  • 등영건 (연세대학교 원주의과대학 환경의생물학교실) ;
  • 이규재 (연세대학교 원주의과대학 환경의생물학교실)
  • Received : 2009.10.14
  • Accepted : 2009.12.24
  • Published : 2009.12.31
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Abstract

This study was performed using animals to confirm the effect of tourmaline-ionized water (TIW) the properties of which were changed by tourmaline energy and electric discharge. In the ICR mice fed high-fat diet, body weight increasing rate of the TIW-treated group (Exp) was generally decreased and moreover exhibited significance at 11th week (P<0.05) compared with the control (Con) group fed distilled water, although water intake of the Exp group was lower than that of the Con group. In the ICR mice with $CCl_4$-induced hepatotoxicity, AST and ALT activities of the Exp group were not significant but showed some decreasing trend, and histological damage of liver was less compared with thatof the Con group. On the study of ethanol-induced hangovers in Sprague-Dawley rat, blood alcohol concentration was significantly decreased (P<0.01), activity of GST, antioxidant enzyme related to the alcohol metabolism, was increased in liver tissue (P<0.05), and AST and ALT show a tendency to be decreasedin the Exp group. These results suggest that drinking TIWhas not only some obesity preventing effect but also an alcohol detoxification effect and liver protecting effect in vivo. It is supposed due to a structural change of water cluster and a property which maintains the changed structure through tourmaline energy and electric discharge. Therefore, TIW has a potentiality to be developed as functional water with several beneficial effects as well as for daily drinking, but further study on the mechanism related with efficacy will be necessary.

본 연구는 투어마린 소재와 전기적인 방전을 통해 물의 성질을 변화시킨 투어마린이온활성수를 이용하여 간보호 효과, 비만 예방 효과, 숙취제거 효과를 확인하기 위해 시행되었다. 고지방식이로 비만을 유도한 ICR 마우스에서 투어마린이온활성수를 급이한 실험군은 정수물을 급이한 대조군과 비교하여 사료섭취량이 비슷하고 음수량이 더 적었지만 체중증가는 대조군에 비하여 억제되어 13주에는 유의한 차이를 보여줌으로써 비만 예방 효과를 나타내었다(P<0.05). 사염화탄소로 간독성을 유발시킨 생쥐를 이용하여 간보호 효과를 관찰한 실험에서, 실험군의 aspartate aminotransferase (AST)와 alanine aminotransferase (ALT)는 대조군과 비교하여 평균적인 차이를 나타내었으며, 간의 조직학적 관찰에서 괴사를 비롯한 병리학적 병변이 감소한 것으로 나타났다. S-D rats을 이용한 숙취 실험에서는 실험군이 대조군에 비해 혈중알콜농도가 유의하게 낮았으며(P<0.01), 알콜 대사와 관련된 항산화 효소인 glutathione-S transferase (GST)는 활성이 증가한 것으로 관찰되었고(P<0.05), 간기능 지표인 AST와 ALT는 상대적인 감소현상을 보였다. 이와 같은 결과는 투어마린이온활성수의 음용이 동물의 항산화 체계에 영향을 미침으로써 간조직을 보호하고 알콜 분해를 향상시킨 것으로 판단되며, 그 결과 숙취해소에 어느 정도 효과가 있을 것으로 판단된다. 투어마린이온활성수의 효능과 관련된 기전은 아직 과학적 검증에 어려움이 있으나, 물에 전달된 투어마린의 원적외선에너지나 전기적 영향으로 인하여 물분자의 결합력과 분자 구조 또는 클러스터의 크기에 변화를 일으켰기 때문으로 판단된다. 사용된 투어마린이온활성수는 음용안전성이 확보된 물로서 효과적인 기능수로 개발될 가능성을 가지며, 유효성과 관련된 기전은 더 연구될 필요성이 있다.

Keywords

References

  1. Bender T, Bariska J, Vaghy R, Gomez R, Kovacs I: Effect of Balneotherapy on the antioxidant system-A controlled pilot study. Arch Med Res 38 : 86-89, 2007 https://doi.org/10.1016/j.arcmed.2006.08.007
  2. Cha JM, Hong SH, Kim SY, Park JY, Kim MS, Lee IH: Cultivation of sprout by highly concentrated oxygen water soaking. Korean J Biotechnol Bioeng 23(6) : 525-528, 2008
  3. Garczarek F, Gerwert K: Functional waters in intraprotein proton transfer monitored by FTIR difference spectroscopy. Nature 439(5) : 109-112, 2006 https://doi.org/10.1038/nature04231
  4. Ham SS, Kim SH, Moon SY, Jeon MS, Oh DH, Cui CB: Antioxidative, antimutagenic and cytotoxic effects of the mineral water. J Fd Hyg Safety 20(1) : 53-57, 2005
  5. Han SM, Yoon DH, Song HM: Effect of strength increase of the concrete mixed with magnetized water. Korean J Civil Eng 21(3A) : 373-379, 2001
  6. Hanaoka K: Antioxidant effects of reduced water produced by electrolysis of sodium chloride solution. J appl Electrochem 31 : 1307-1313, 2001 https://doi.org/10.1023/A:1013825009701
  7. Her E, Jun TS, Cho IH, Park DK: Scientific assesment of the natural environmental resources for the leisure in the hot-spring resorts of jungwon: research for the component of the hot-spring Suanbo. Konkuk Nat Sci Res 9(1) : 171-190, 1998
  8. Huang HC, Jupiter D, Qiu M, Briggs JM, VanBuren V: Cluster analysis of hydration waters around the active sites of bacterial alanine racemose using 2-ns MD stimulation. Biopolymers 89(3) : 210-219, 2008 https://doi.org/10.1002/bip.20893
  9. Inoue S, Honda K: Growth of rats exposed to far-infrared radiation. Zool Sci 3 : 731-732, 1986
  10. Inoue S, Kabaya M: Biological activities caused by far-infrared radiation. Int J Biometeorol 33 : 145-150, 1989 https://doi.org/10.1007/BF01084598
  11. Ise N, Katsuura T, Kikuchi Y, Miwa E: Effect of far-infrared radiation on forearm skin blood flow. Ann Physiol Anthrop 6 : 31-32, 1987 https://doi.org/10.2114/ahs1983.6.31
  12. Itokawa Y: An overview on researches of potable alkaline water by electrolysis. J Funct Water 2(2) : 59-64, 2004
  13. James T, Wales DJ, Hernaddex Rojas J: Energy landscapes for water clusters in a uniform electric field. J Chem Phys 126(054506):1-13, 2007
  14. Jang JO, Lee YM: A study on availability of magnetic treatment water as a cooking water. Korean J Dietary Culture 11(1) : 37-42, 1996
  15. Jin D, Park SK, Lee YM, Yoon YS, Kim DH, Deung YK, Lee KJ: Effect of Mineral induced alkaline reduced water on Sprague Dawley rats fed on high-fat diet. Korean J Biomed Lab Sci 12(1): 1-7, 2006
  16. Keutsch FN, Saykally RJ: Water clusters: untangling the mysteries of the liquid, one molecule at a time. PNAS 98(19) : 10533-10540, 1999 https://doi.org/10.1073/pnas.191266498
  17. Kim JW, Kim KH, Park DK, Lee KJ: The glutathione S-transferase activity of alkaline reduced water (ARW) on Sprague-Dawley rats. Newest Med J 46(10) : 111-116, 2003
  18. Kim TY, Kim GY, Lambeck J: Hydrotherapy in rheumatoid arthritis. J of Korean Soc Phys Ther 12(3) : 407-414, 2000
  19. Kim YD, Chung IM, Lee KG: Silk degumming by electrolyzed alkaline water. Korean J Seric Sci 47(1) : 36-40, 2005
  20. Lee IH, Youn JI: Pain relief and satisfaction by hydrotherapy among urban elderly. J Korean Soc phys ther 14(3) : 209-216, 2002
  21. Liang J, Zhu D, Meng J, Wang L, Li F, Liu Z, Ding Y, Liu L, Liang G: Performance and application of far infrared rays emitted from rare earth mineral composite materials. J Nanosci Nanotechnol 8(3) : 1203-1210, 2008
  22. Ludwig R: Water: From clusters to the bulk. Angew Chem Int Ed 40 : 1808-1827, 2001 https://doi.org/10.1002/1521-3773(20010518)40:10<1808::AID-ANIE1808>3.0.CO;2-1
  23. Matz H, Orion E, Wolf R: Balneotherapy in dermatology. Dermatol Ther 16 : 132-140, 2003 https://doi.org/10.1046/j.1529-8019.2003.01622.x
  24. Meng J, Liang J, Ou X, Ding Y, Liang G: Effects of mineral tourmaline particles on the photocatalytic activity of $TiO_2$ thin films. J Nanosci Nanotichnol 8(3) : 1279-1283, 2008
  25. Niwa Y, Iizawa O, Ishimoto K, Jiang X, Kanoh T: Electromagnetic wave emitting products and 'Kikoh' potentiate human leukocyte functions. Int J Biometeorol 37 : 133-138, 1993 https://doi.org/10.1007/BF01212622
  26. Nocco PB: Mineral water as a cure. Verpff Schweiz Ges Gesch Pharm 29 : 13-402, 2008
  27. Paek UH, Jeong ED, Yun CK: The characteristics of water quality of tap water and far-infrared rays mineral water. J Korean Environ Sci 9(5) : 423-428, 2000. (Korean)
  28. Pocker Y: Water in enzyme reactions: biophysical aspects of hydration-dehydration process. Cell Mol Life Sci 57 : 1008-1017, 2000 https://doi.org/10.1007/PL00000741
  29. Rai D, Kulkarni AD, Gejji SP, Pathak RK: Water clusters ($H_2O$)n, n=6-8, in external electric fields. Chem Phys 128(034310) : 1-14, 2008
  30. Tamasidze A: Biochemical results of radon treatment. Georgian Med News 141 : 91-93, 2006
  31. Xia M, Zhang H, Hu C, Xu Z: Regulation effects of tourmaline on seawater pH value. Ying Yong Sheng Tai Xue Bao 16(10) : 1972-1977, 2005
  32. Yamaoka K, Mifune T, Mitsunobu F, Kojima S, Mori S, Shibuya K, Tanizake Y, Sugita K: Basic study on radon effects and thermal effects on humans in radon therapy. Physiol Chem Phys Med NMR 33(2) : 133-138, 2001
  33. Yoshizawa Y, Kitamura K, Kawana S, Maibach HI: Water, salts and skin barrier of normal skin. Skin Res Technol 9 : 31-33, 2003 https://doi.org/10.1034/j.1600-0846.2003.00346.x