YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Proteomic Approach to the Cytotoxicity of 5-FU(Fluorouracil) in Colon Cancer Cells

대장암 세포에서 5-FU(Fluorouracil)의 세포독성과 관련된 단백체 분석

  • Lee, Seo-Young (College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University) ;
  • Song, Jin-Su (Department of Chemistry, Seoul National University) ;
  • Roh, Si-Hun (College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University) ;
  • Kim, Geun-Tae (College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University) ;
  • Hong, Soon-Sun (College of Medicine (BK21), Inha University) ;
  • Kim, Hie-Joon (Department of Chemistry, Seoul National University) ;
  • Kwon, Sung-Won (College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University) ;
  • Park, Jeong-Hill (College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University)
  • Published : 2009.06.30
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Abstract

We evaluated cytotoxic effect based on the MTT assay and identified altered proteins in 5-FU(fluorouracil) treated HT29 cells using two-dimensional gel electrophoresis and MALDI-TOF/TOF-MS. As proteins inducing apoptosis, siah binding protein 1 and p47 protein isoform a were up-regulated and tumor protein translationally-controlled 1 was down-regulated by 5-FU treatment. And mannose 6 phosphate receptor binding protein 1 controls DNA mismatch repair system was increased. We suggest 5-FU promotes a cytotoxicity under the action of these proteins in colon cancer cells.

Keywords

References

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