The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
권호 표지

Neuroprotective Effect of Methanol Extract of Phellodendri Cortex Against 1-methyl-4-Phenylpyridinium-induced Apoptosis in PC-12 Cells

1-methyl-4-phenylpyridinium($MPP^+$)로 유도된 파킨슨병의 세포손상에 대한 황백의 신경세포 보호효과

  • Jung, Young-Seok (Dept. of Oriental Internal Medicine, College of Oriental Medicine, Dong-guk University) ;
  • Jung, Hye-Mi (Dept. of Oriental Internal Medicine, College of Oriental Medicine, Dong-guk University) ;
  • Seo, Un-Kyo (Dept. of Oriental Internal Medicine, College of Oriental Medicine, Dong-guk University)
  • 정영석 (동국대학교 한의과대학 내과학교실) ;
  • 정혜미 (동국대학교 한의과대학 내과학교실) ;
  • 서운교 (동국대학교 한의과대학 내과학교실)
  • Published : 2009.03.31
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Abstract

Background and Objective : The prospects for developing an anti-apoptotic natural component or a compound that exerts a neuroprotective effect with few or no side effects for the treatment of neurodegenerative disease appear favorable. In the present study, we evaluated the effects of the methanol extract of Phellodendri Cortex (PC extract) on 1-methyl-4-phenyl pyridinium($MPP^+$)-induced apoptosis in PC-12 cells. Materials and Methods : We used the methanol extract of Phellodendri Cortex (PC extract). PC-12 cells were cultured by RPMZ-1640. We found the PC extract's gene expression (Bax, Bcl-2) by using RT-PCR. We examined the PC extract's protein expression (Bcl-2, Bax, cytochrome c, poly (ADP-ribose) polymerase (PARP), caspase-3) by SDS-PAGE and Western blot. Results : Apoptosis in $MPP^+$-induced PC-12 cells was accompanied by an increased Bax/Bcl-2 ratio, release of cytochrome c to the cytosol and the activation of caspase-3. PC extract inhibited the down-regulation of Bcl-2 and the up-regulation of Bax, as well as the release of mitochondrial cytochrome c into the cytosol. In addition, PC extract attenuated caspase-3 activation and cleavage of poly (ADP-ribose) polymerase (PARP). Conclusion : These results suggest that the neuroprotective potentials of PC extract against $MPP^+$-induced apoptosis can be. at least partially, ascribed to its anti-apoptotic effects in PC-12 cells.

Keywords

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