Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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The Effects of Aqueous Fibrolytic Enzyme Products Supplementation on In vitro Fermentation and Growth Performance of Hanwoo Steers

섬유소분해효소 발효액의 첨가가 In vitro 발효 및 거세한우의 성장에 미치는 영향

  • Kook, Kil (Division of Animal Science, Chonnam National University) ;
  • Chung, Wan-Tae (National Institute of Animal Science, RDA) ;
  • Park, Hong-Seok (Korea Research Institute of Bioscience & Biotechnology) ;
  • Jang, Ki-Yeoung (Division of Animal Science, Chonnam National University) ;
  • Lee, Ji-Wung (Division of Animal Science, Chonnam National University) ;
  • Moon, Seung-Ju (Division of Animal Science, Chonnam National University) ;
  • Kim, Kwang-Hyun (Division of Animal Science, Chonnam National University)
  • 국길 (전남대학교 동물자원학부) ;
  • 정완태 (농촌진흥청 국립축산과학원) ;
  • 박홍석 (한국생명공학연구원) ;
  • 장기영 (전남대학교 동물자원학부) ;
  • 이지웅 (전남대학교 동물자원학부) ;
  • 문승주 (전남대학교 동물자원학부) ;
  • 김광현 (전남대학교 동물자원학부)
  • Received : 2007.12.31
  • Accepted : 2009.01.08
  • Published : 2009.02.01
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Abstract

The objective of this study was to investigate the effects of an enriched diet supplemented with aqueous fibrolytic enzyme products (FEP) on In vitro fermentation and growth performance of Hanwoo Steers. Total mixed ration combined with fibrolytic enzyme products showed a slight increase in the pH level on In vitro fermentation. Acetate ratio in volatile fatty acids, and the concentration of total volatile fatty acids were significantly greater at 12hr and 24hr after cultivation (p<0.05). Dry matter digestibility was significantly increased in groups supplied with aqueous fibrolytic enzyme products at 24hr after cultivation (p<0.05). In a rearing trial, the average daily gain and feed efficiency improved in the treatment groups supplied with aqueous fibrolytic enzyme products. Nutrient digestibility such as DM, crude fiber, NDF, and ADF digestibility were also significantly increased with the addition of aqueous fibrolytic enzyme products (p<0.05). Therefore, the aqueous fibrolytic enzyme products supplementation on In vitro fermentation is effective in improving the condition of rumainal fermantation. Also two kinds of aqueous fibrolytic enzyme products were found to be effective in improving the average daily gain and feed efficiency in Hanwoo Steers.

본 연구는 목재부후균 유래 $\beta$-글루코시다제, 엑소글루카나제 및 엔도글루카나제 유전자를 함유한 형질전환체 효모를 배양한 액상섬유소분해효소에 대하여 인공반추위 배양시험을 통해 완전혼합사료에 대한 적용 수준 조사와 더불어 한우 비육 거세우의 사양시험을 통해 2 종류의 액상섬유소분해효소를 완전혼합사료에 1.0% 수준으로 첨가 급여하여 가축의 성장, 영양소소화율에 미치는 영향을 알아보고자 실시하였다. 1. 인공반추위 배양시험을 통해 완전혼합사료에 대한 액상 섬유소분해효소 첨가에 의해 pH가 약간 증가하는 경향이었다. 휘발성지방산에서 acetate 비율이 배양 12와 24시간에 유의적으로(p<0.05) 높게 나타났으며 총휘발성지방산 농도 역시 배양 12와 24시간에 유의적으로(p<0.05) 증가시켰으며 1.0% 첨가구에서 효과적이었다. 건물소화율은 배양 24시간에 액상 섬유소분해효소 첨가구에서 유의적으로(p<0.05) 증가하였다. 2. 한우 거세우 사양시험에서는 액상 섬유소분해효소 급여에 의해 일당증체량과 사료효율이 개선되는 경향이었다. 영양소 소화율에서는 DM, 조섬유, NDF 및 ADF 소화율이 액상 섬유소분해효소 첨가에 의해 유의적으로(p<0.05) 증가하였다. 따라서 인공반추위 배양시험을 통해 액상 섬유소분해효소 1.0% 첨가가 비교적 반추위발효 양상을 개선시키었으며, 한우 사양시험에서도 2종류의 액상 섬유소분해효소 1.0% 급여가 일당증체량 및 사료효율 개선효과가 있었다.

Keywords

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