Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Experimental Studies on the Hair Growth Activity of Fractions and Extract of Arisaematis Rhizoma in C57B/6N Mice

C57BL/6N 생쥐에서 천남성 추출물과 분획물의 발모효과에 대한 실험적 연구

  • Kwon, Kyung-Suk (Department of sasang Constitutional Medicine, College of Oriental Medicine, Woosuk University) ;
  • Lee, Moon-Won (Department of Anatomy, College of Oriental Medicine, Woosuk University) ;
  • Jeong, Il-Kook (Department of Anatomy, College of Oriental Medicine, Woosuk University) ;
  • Jeong, Han-So (Department of Pathology, College of Oriental Medicine, Woosuk University) ;
  • Song, Beom-Yong (Department of Acupuncture & Moxibution, College of Oriental Medicine, Woosuk University) ;
  • Song, Jeong-Mo (Department of sasang Constitutional Medicine, College of Oriental Medicine, Woosuk University) ;
  • Lee, Chang-Hyun (Department of Anatomy, College of Oriental Medicine, Woosuk University)
  • 권경숙 (우석대학교 한의과대학 사상체질의학교실) ;
  • 이문원 (우석대학교 한의과대학 해부학교실) ;
  • 정일국 (우석대학교 한의과대학 해부학교실) ;
  • 정한솔 (우석대학교 한의과대학 병리학교실) ;
  • 송범용 (우석대학교 한의과대학 침구학교실) ;
  • 송정모 (우석대학교 한의과대학 사상체질의학교실) ;
  • 이창현 (우석대학교 한의과대학 해부학교실)
  • Published : 2009.06.25
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Abstract

To investigate the hair growth activity of fractions and extract of Arisaematis Rhizoma in the hair removed skin of normal and spontaneous alopecia areata model in C57B/6N mice. These experiments were performed with the macroscopic, microscopic, immunohistochemical(VEGF, c-kit, PKC-${\alpha}$, TGF and FGF) and RT-PCR(TGF-${\beta}$, IGF, prolactin and placenta lactogen) methods. The results were as follows: Macroscopic observation after topical application of vehicle, 50% EtOH as control and extract of Arisaematis Rhizoma to the hair removed skin of C57BL/6N mice on the 9th, 11th and 15th day. Extensive hair growth activity was observed in treated group with extract of Arisaematis Rhizoma on the 9th, 11th and 15th day. In Arisaematis Rhizoma extracts treated group, hair follicles of middle stage of anagen was observed and it were grown down to subcutaneous tissue of skin in all the normal mice on 15th day. But in control group, most of hair follicles of telogen phase was observed in skin. The treatment of extract of Arisaematis Rhizoma increased expression of IGF(145%) and placenta lactogen(108%) in the skin of normal C57BL/6N mice on the 11th day compared to control group(100%). But expression of TGF-${\beta}$(90%) and prolactin(91%) decreased in the skin of normal C57B/6N mice on the 11th day compared to control group(100%). After application of fractions(chloroform, ethyl acetate and water fractions) of Arisaematis Rhizoma extract for 9th day, hair growth effect was observed in whole skin area in 50% of normal mice. But in control group, hair growth effect was not observed in whole skin area of normal mice. Immunoreactive density of VEGF, c-kit, PKC-${\alpha$ and FGF in skin of fractions of Arisaematis Rhizoma extracts was strongly stained in epidermis, bulge, secondary hair germ cells, cutaneous trunci m., subcutaneous tissue, root sheath compare to control group on the 9th day. In spontaneous alopecia areata model, The hair growth activity of Arisaematis Rhizoma extrat treated group(75%) was observed to be strong compared to control group(O%) on 7th day. These experiments suggest that fractions and extracts of Arisaematis Rhizoma may stimulate the topical hair growth activity. Thus it can be useful for treatment of alopecia areata.

Keywords

References

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