DOI QR코드

DOI QR Code

Changes in the Composition of Catechins, Theaflavins and Alkaloids in Leaves from Korean Yabukida Tea Plant During Processing to Fermented Black Tea

한국산 야부끼다종 차엽으로 만든 홍차 제조과정 중의 catechins, theaflavins, alkaloids 함량 변화에 관한 연구

  • Choi, Suk-Hyun (Department of Food Service Industry Seowon University, Bio Organic Material & Food Center Seowon University)
  • 최석현 (서원대학교 외식산업학과, 서원대학교 BioRIC)
  • Published : 2009.06.30

Abstract

In this study, we examined the composition of catechins, theaflavins and alkaloids in leaves during processing to fermented black tea, which is produced by withering, roll breaking, and fermentation of Korean Yabukida tea plant. In addition, we determined the optimal conditions for the production of fermented black tea. The average moisture content in fresh leaves was 70.85%, which dropped to 3.07% in fermented black tea at the last stage of production. When the leaves were analyzed by HPLC, seven types of catechins, four types of theaflavins and three types of alkaloids were identified. The levels of catechins, theaflavins, and alkaloids were then evaluated after being processed into fermented tea. From these experiments, we found that the level of theaflavins, which determines the property of the tea, increased during fermentation. This effect resulted from the change in EGCG, ECG, EGC, EC during the process of fermentation. We also found that the maximal amount of theaflavins was created after 1-2 hours of fermentation. Thus, our results imply that the best condition for producing fermented black tea would be to ferment for 1-2 hours.

Keywords

References

  1. 中莖秀夫, 竹內啓子, 正樹, 藤井正人. 2002. 茶樹の秋季整 枝葉の發酵加工について. 愛知食品工業技術センタ 一究報告書. pp 1-4
  2. AOAC. 1965. Methods of Analysis. Washington, DC: Association of Official Analytical Chemists, pp 361
  3. Borrelli F, Capasso R, Russo A, Ernst E. 2004. Systematic review: green tea and gastrointestinal cancer risk. Aliment Pharmacol Ther, 19(5):497-510 https://doi.org/10.1111/j.1365-2036.2004.01884.x
  4. Cui Y, Morgenstern H, Greenland S, Tashkin DP, Mao JT, Cai L, Cozen W, Mack TM, Lu Q-Y, Zhang Z-F. 2008. Dietary flavonoidin take and lung cancer- Apopulation-based casecontrolstudy. Cancer, 112(10):2241-2248 https://doi.org/10.1002/cncr.23398
  5. Friedman M. 2007. Overview of antibacterial, antitoxin, antiviral, and antifungal activities of tea flavonoids and teas. Mol. Nutr. Food Res., 51(1):116-134 https://doi.org/10.1002/mnfr.200600173
  6. Friedman M, Henika PR, Levin CE, Mandrell RE, Kozukue N. 2006. Antimicrobial activities of tea catechins and the aflavins and tea extracts against Bacilluscereus. J. Food Prot., 69(2):354-361 https://doi.org/10.4315/0362-028X-69.2.354
  7. Friedman M, Mackey BE, Kim HJ, Lee IS, Lee KR, Lee SU, Kozukue E, Kozukue N. 2007. Structure-activity relationships of tea compounds against human cancer cells. J. Agric. Food Chem., 55(2):243-253 https://doi.org/10.1021/jf062276h
  8. Higdon JV, Frei B. 2003. Tea catechins and polyphenols:health effects, metabolism, and antioxidant functions. Crit. Rev. Food Sci. & Nutr., 43(1):89-143 https://doi.org/10.1080/10408690390826464
  9. Hollman PC, Feskens EJ, Katan MB. 1999. Tea flavonols in cardiovascular disease and cancer epidemiology. Proceedings of Soc. Exp. Biol. & Med., 220(4):198-202 https://doi.org/10.1046/j.1525-1373.1999.d01-33.x
  10. He Q, Yao K, Jia D, Fan H, Liao X, Shi B. 2009. Determination of total catechins in tea extracts by HPLC and spectrophotometry. Nat. Prod. Res., 23(1):93-100 https://doi.org/10.1080/14786410801886682
  11. Inami S, Takano M, Yamamoto M, Murakami D, Tajika K, Yodogawa K, Yokoyama S, Ohno N, Ohba T, Sano J, Ibuki C, Seino Y, Mizuno K. 2007. Tea catechin consumption reduces circulating oxidized low-density lipoprotein. Int. Heart J., Nov; 48(6):725-732 https://doi.org/10.1536/ihj.48.725
  12. Kotani A, Takahashi K, Hakamata H, Kojima S, Kusu F. 2007. Attomole catechins determination by capillary liquid chromatography with electrochemical detection. Anal. Sci.,23(2):157-163 https://doi.org/10.2116/analsci.23.157
  13. Liang YC, Chen YC, Lin YL, Lin-Shiau SY, Ho CT, Lin JK. 1999. Suppression of extracellular signals and cell proliferation by the black tea polyphenol, the aflavin-3,$3^{\prime}$-digallate. Carcinogenesis, 20(4):733-736 https://doi.org/10.1093/carcin/20.4.733
  14. Patel R, Krishnan R, Ramchandani A, Maru G. 2008. Polymeric black tea polyphenols inhibit mouse skin chemical carcinogenesis by decreasing cell proliferation. Cell Proliferation, 41(3):532-553 https://doi.org/10.1111/j.1365-2184.2008.00528.x
  15. Sano M, Tabata M, Suzuki M, Degawa M, Miyase T, Maeda-Yamamoto M. 2001. Simultaneous determination of twelve tea catechins by high-performance liquid chromatography with electrochemical detection. Analyst, 126(6):816-820 https://doi.org/10.1039/b102541b
  16. Scalbert A, Manach C, Morand C, Remesy C, Jimenez L. 2005. Dietary polyphenols and the prevention of disease. Crit. Rev. in Food Sci. & Nutr., 45(4):287-306 https://doi.org/10.1080/1040869059096
  17. Tanaka T, Matsuo Y, Kouno I. 2005. A novel black tea pigment and two new oxidation products of epigallocatechin-3-O-gallate. J. Agric. Food Chem., 53(19):7571-7578 https://doi.org/10.1021/jf0512656
  18. Tanaka T, Mine C, Watarumi S, Fujioka T, Mihashi K, Zhang Y-J, Kouno I. 2002. Accumulation of epigallocatechin quinone dimers during tea fermentation and formation of theasinensins. J. Nat. Prod., 65(11):1582-1587 https://doi.org/10.1021/np020245k
  19. Tinahones FJ, Rubio MA, Garrido-S?nchez L, Ruiz C, Gordillo E, Cabrerizo L, Cardona F. 2008. Green tea reduces LDL oxidability and improves vascular function. J. Am. Coll Nutr., 27(2):209-213 https://doi.org/10.1080/07315724.2008.10719692
  20. Zuo Y, Chen H, Deng Y. 2002. Simultaneous determination of catechins, caffeine and gallic acids in green, Oolong, black and pu-erh teas using HPLC with a photodiode array detector. Talanta, 57(2):307-316 https://doi.org/10.1016/S0039-9140(02)00030-9