Journal of Oriental Neuropsychiatry (동의신경정신과학회지)

The Korean Society of Oriental Neuropsychiatry (대한한방신경정신과학회)
권호 표지

The Effect of an Essential Oil Fragrance from Radix Angelica Sinesis on Differentiation and Proliferation of Neural Stem Cells of Rat

당귀(當歸) 향기액(香氣液)이 Rat의 뇌신경줄기세포의 분화(分化)와 증식(增殖)에 미치는 영향

  • Park, Se-Hwan (Dept. of Neuropsychiatry, College of Korean Medicine, Dong-Guk University) ;
  • Kang, Jae-Hyun (Dept. of Neuropsychiatry, College of Korean Medicine, Dong-Guk University) ;
  • Jung, Young-Su (Dept. of Neuropsychiatry, College of Korean Medicine, Dong-Guk University) ;
  • Kim, Geun-Woo (Dept. of Neuropsychiatry, College of Korean Medicine, Dong-Guk University) ;
  • Koo, Byung-Soo (Dept. of Neuropsychiatry, College of Korean Medicine, Dong-Guk University)
  • 박세환 (동국대학교 한의과대학 신경정신과학교실) ;
  • 강재현 (동국대학교 한의과대학 신경정신과학교실) ;
  • 정영수 (동국대학교 한의과대학 신경정신과학교실) ;
  • 김근우 (동국대학교 한의과대학 신경정신과학교실) ;
  • 구병수 (동국대학교 한의과대학 신경정신과학교실)
  • Received : 2009.11.06
  • Accepted : 2009.12.01
  • Published : 2009.12.30
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Abstract

Objectives : In this study, an essential oil fragrance from Danggwi was administrated into the neural stem cell and the effect of the essential oil on the differentiation and proliferation of the neural stem cells were observed. Methods : The establishment of the neural stem cell was identified via Nestin, DAPI dye. An essential oil fragrance from Danggwi was administrated with a proved optimum level for the survival of the cell through MTT assay. Also, according to the analysis of Western blot, the essential oil fragrance from Danggwi promotes the phosphorylating of Akt, Erk, ERM protein. Results : MTT assay showed increased in GFAP. The result indicates that the differentiation to astrocyte is promoted. The phosphorylation levels of ERM, Erk and Akt were increased at 60 min after addition of 5 ug/ml of essential oil fragrance from Danggwi and sustained to 48 hours. These imply that essential oil fragrance from Danggwi may induce the survival and the proliferation of the differentiated cells. Conclusions : These results suggest that the essential oil fragrance from Danggwi can be effective for the in vivo study of degenerative neuronal disease using neural stem cell.

Keywords

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