Korean Journal of Soil Science and Fertilizer (한국토양비료학회지)

Korean Society of Soil Science and Fertilizer (한국토양비료학회)
권호 표지

Effect of Temperature on Soil Microbial Biomass, Enzyme Activities, and PLFA content during Incubation Period of Soil Treated with Organic Materials

유기물원 항온배양 온도가 토양미생물체량과 효소활성 및 PLFA함량에 미치는 영향

  • Joa, Jae-Ho (National Institute of Horticultural & Herbal Science, RDA) ;
  • Moon, Doo-Gyung (National Institute of Horticultural & Herbal Science, RDA) ;
  • Chun, Seung-Joung (National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Chun-Hwan (National Institute of Horticultural & Herbal Science, RDA) ;
  • Choi, Kyung-San (National Institute of Horticultural & Herbal Science, RDA) ;
  • Hyun, Hae-Nam (Major of Plant Resources and Environment, Jeju National University) ;
  • Kang, Ui-Gum (National Institute of Crop Science, RDA)
  • Received : 2009.10.20
  • Accepted : 2009.11.19
  • Published : 2009.12.30
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Abstract

This study was carried out to evaluate the effect of temperature on soil microbial biomass, enzyme activities, and PLFA content in the volcanic(VAS) and the non-volcanic ash soil(NVAS). The soils were treated with organic materials such as organic fertilizer pelleted(OFPL), organic fertilizer powdered(OFPD), pig manure compost(PMC), and food waste compost(FWC). Two grams of organic materials were well mixed with 30g of dried volcanic and non-volcanic ash soil(< 2 mm) with 50% of soil moisture content. And the soils were incubated at 10, 20, $30^{\circ}C$ in incubator. Soils were analysed on the incubation times as followed; soil pH, total nitrogen, organic matter(at 75, 150, 270 days), microbial biomass C and PLFA (at 75, 270 days), microbial biomass N and soil enzyme(at 150, 270 days). pH values of soils treated with PMC and FWC had no changes on soil type, and incubation temperature. However, the pH was increased with temperature in the soils treated with OFPL. The changes in NVAS was higher than in VAS. Soil microbial biomass C content were high in the condition of high temperature and organic fertilizers treatment in VAS. But the contents were gradually decreased with incubation period in both NVAS and VAS. Soil microbial biomass N was high in NVAS treated with organic fertilizers and in VBS treated with PMC and FWC. PLFA content was higher in NVBS than in VBS at 75 days but showed high in VBS at 270 days. Urease activity of NVBS treated with OFPL showed $10^{\circ}C$ (75.0)> $20^{\circ}C$ (16.3)>$30^{\circ}C$ ($4.6ug\;NH{_4-}N\;g^{-1}\;2h^{-1}$) at 150 days. It were decreased gradually high temperature and time passes. And it showed high at $10^{\circ}C$ in VBS. Glucosidase activity was higher in NVBS than in VBS. Correlation coefficient of between soil microbial biomass C and microbial activity indicators showed that PLFA was high significantly at $r^2=0.91$ in NVBS and ${\beta}-glucosidase$ was $r^2=0.83$ in VBS. Soil microbial activities showed differences in the relative sensitivities of soil type and soil temperature.

유기물원을 항온 배양했을 때 온도가 토양미생물체량과 효소활성 및 PLFA함량에 미치는 영향을 토양 특성별로 평가하고자 입상과 분상 혼합유기질비료, 돈분퇴비, 음식물퇴비를 화산회토양과 비화산회토양 30g에 2g을 잘 혼합 후 10, 20, $30^{\circ}C$에서 항온배양을 하면서 pH, 토양질소, 유기물 함량, Microbial biomass C와 N, 토양효소활성, 인지질지방산 함량을 분석하였다. 돈분과 음식물퇴비는 토양종류와 온도에 상관없이 토양 pH의 변화가 크지 않았으나 입상유기질비료는 온도가 높을수록 낮아졌으며, 비화산회토양에서 변화폭이 컸다. 미생물체량 C는 화산회토양의 경우 배양 온도가 높고 유기질비료를 처리할 때 높아지는 경향이었지만 토양종류에 상관없이 점차 감소하였다. 미생물체량 N은 비화산회토양에서 유기질비료, 화산회토양에서는 돈분과 음식물퇴비를 처리할 때 높게 나타났다. 인지질 지방산함량은 항온배양 75일후 비화 산회토양이 화산회토양보다 높았고, 270일후 화산회토양에서 높게 나타났으나 점차 낮아지는 경향이었다. Urease활성은 150일에 비화산회토양의 입상유기질비료처리에서 $10^{\circ}C$(75.0)>$20^{\circ}C$(16.3)>$30^{\circ}C$($4.6ug\;NH{_4-}N\;g^{-1}\;2h^{-1}$)순으로 $10^{\circ}C$에서 가장 높게 나타났으며 온도가 높고, 시간이 경과할수록 낮아졌으며 화산회토양의 Urease활성은 $10^{\circ}C$에서 높게 나타났다. ${\beta}-glucosidase$ 활성은 비화산회토양이 화산회토양보다 높았고 시간이 경과할수록 낮아졌다. 토양미생물체량 C와 미생물활성지표간의 상관계수는 비화산회토양에서 PLFA ($r^2=0.91$), 화산회토양에서 ${\beta}-glucosidase$ ($r^2=0.83$)가 높았으며, 미생물활성지표는 토양종류와 온도에 따라 상대적으로 민감도가 다르게 나타났다.

Keywords

References

  1. Anderson, D.W. 1979. Processes of humus formation and transformation in soils of the Canadian Great Plains. Euro. J. Soil Sci. 30:77-84 https://doi.org/10.1111/j.1365-2389.1979.tb00966.x
  2. Anderson, J.M. 1991. The effects of climate change on decomposition processes in grassland and coniferous forests. Ecol.Appl. 1:326 347 https://doi.org/10.2307/1941761
  3. Agehara, S. and D.D. Warncke. 2005. Soil moisture and temperature effects on nitrogen release from organic nitrogen sources. AM. J.Soil Sci. Soc. 69:1844-1855 https://doi.org/10.2136/sssaj2004.0361
  4. Amato, M. and J.N. Ladd. 1988. Assay for microbial biomass based on Ninhydrin -reactive nitrogen in extracts of fumigated soils. Soil Biol. Biochem. 20:107-I14 https://doi.org/10.1016/0038-0717(88)90134-4
  5. Alvarez, R., O.J. Santanatoglia, and R. Garcja. 1995. Effect of temperature on soil microbial biomass and its metabolic quotient in situ under different tillage systems. Biol. Fertil. Soils. 19(2):227-230 https://doi.org/10.1007/BF00336164
  6. Baath, E. and T.H. Anderson. 2003. Comparison of soil fungal/bacterial ratios in a pH gradient using physiological and PLFA-based techniques. Soil Biol. Biochem. 35(7):955-963 https://doi.org/10.1016/S0038-0717(03)00154-8
  7. Bligh, E.G. and W.J. Dyer. 1959. A rapid method for total lipid extraction and purification. Can. J. Biochem. Physiol. 37:911-917 https://doi.org/10.1139/o59-099
  8. Bremner, J.M and R.L. Mulvaney. 1978. Urease activity in soil. In:Burns RG(ed) Soil enzymes. Academic Press, London, pp 149-196
  9. Cerhanova, D., J. Kubat, and J. Novakova. 2006. Respiration activity of the soil samples from the long-term field experiments in Prague. Plant Soil Environ. 52:21-28 https://doi.org/10.1111/j.1747-0765.2006.00005.x
  10. Cordovil, C.M.D.S., J. Coutinho, M. Goss, and F. Cabral. 2005. Potentially mineralizable nitrogen from organic materials applied to a sandy soil: fitting the one-pool exponential model. Soil Use Manage. 21:65-72 https://doi.org/10.1079/SUM2005294
  11. Cookson W.R., M. Osman, P. Marschner, D.A. Abaye, I. Clark,D.V. Murphy, E.A. Stockdale, and C.A. Watson. 2007. Controls on soil nitrogen cycling and microbial community composition across land use and incubation temperature. Soil Biol. Biochem.39:744-756 https://doi.org/10.1016/j.soilbio.2006.09.022
  12. Debosz, K., P.H. Rasmussen, and A.R. Pedersen.1999. Temporal variations in microbial biomass C and cellulolytic enzyme activity in arable soils : effects of organic matter input. Appl. Soil Ecol.13:209-218 https://doi.org/10.1016/S0929-1393(99)00034-7
  13. Deenik, J. 2006. Nitrogen mineralization potential in important agricultural soils of Hawaii. Uni. Hawaii, CTAHR. Soil Crop Manage. 15:1-5
  14. Dick, R. P.1992. A review: long-term effects of agricultural systems on soil biochemical and microbial parameters. Agric. Ecosyst.Environ. 40: 25-36 https://doi.org/10.1016/0167-8809(92)90081-L
  15. Dinesh, R., R.P. Dubey, and G.S. Prasad. 1998. Soil microbial biomass and enzyme activities as influenced by organic manure incorporation into soils of a rice-rice system. J. Agro. Crop Sci.181:173-178 https://doi.org/10.1111/j.1439-037X.1998.tb00414.x
  16. Friedel, J. K. E. Otto, P. Michael, S. Michael, V. Tobias, S.Michael.2006. Soil microbial biomass and activity : the effect of site characteristics in humid temperate forest ecosystems.J. plant nutrition & soil science.169:175-184 https://doi.org/10.1002/jpln.200521763
  17. Goyal S., K. Sakamoto, and K. Inubashi. 2000. Microbial biomass and activities along an andosol profile in relation to soil organic matter level. Microb. Environ. 15:143-150 https://doi.org/10.1264/jsme2.2000.143
  18. Green, C.T. and K.M. Scow. 2008. Analysis of phospholipid fatty acids(PLFA) to characterize microbial communities in aquifers.Hydrogeo. J. 8:126-141
  19. Henmi, T. and K. Wada. 1976. Morphology and composition of allophane. Am. Mineral. 61:379-390
  20. Hu, C. and Z. Cao. 2007. Size and activity of the soil microbial biomass and soil enzyme activity in long-term field experiments. World J. Agri. Sci. 3(1):63-70
  21. Joa, J.H., J.H. Lee, H.Y. Won, S.G. Han, and H.C. Lim. 2008.Effect of different soil managements on physical properties and microbial activities in citrus orchard soil. Korean J. Soil Sci. Fert.41:279-284
  22. Joa, J.H., S.G. Han, H.Y. Won, H.C. Lim, H.N. Hyun, and J.S. Suh.2009. Effect of different fertilization management practices on soil microbial activities and community structure in volcanic ash citrus orchard soil. Korean J. Soil Sci. Fert. 42:222-229
  23. Kao, P.H., C.C. Huan, and Z.Y. Hseu. 2006. Response of microbial activities to heavy metals in a neutral loamy soil treated with biosolid. Chemosphere. 64:63-70 https://doi.org/10.1016/j.chemosphere.2005.11.039
  24. Kelly, J.M. and G.S. Henderson. 1978. Effects of nitrogen and phosphorus additions on deciduous litter decomposition. Am. J.Soil Sci. Soc. 42:972-976 https://doi.org/10.2136/sssaj1978.03615995004200060030x
  25. Kemmitt, S.J., C.V. Lanyon, I.S. Waite, Q. Wen, T.M. Addiscott, N.R.A. Bird, A.G. O'Donnell, P.C. Brookes. 2008. Mineralization of native soil organic matter is not regulated by the size, activity or composition of the soil microbial biomass C new perspective. Soil Biol. Biochem. 40:61-73 https://doi.org/10.1016/j.soilbio.2007.06.021
  26. Kemmitt, S.J., D. Wright, K.W.T. Goulding, and D.L.Jones. 2006. pH regulation of carbon and nitrogen dynamics in two agricultural soils. Soil Biol. Biochem. 38:898-911 https://doi.org/10.1016/j.soilbio.2005.08.006
  27. Kemmitt, S.J., D. Wright, K.W.T. Goulding, and D.L.Jones. 2006. pH regulation of carbon and nitrogen dynamics in two agricultural soils. Soil Biol. Biochem. 38:898-911 https://doi.org/10.1016/j.soilbio.2005.08.006
  28. Kirschbaum, M.U.F. 1995. The temperature dependence of soil organic matter decomposition, and the effect of global warming on soil organic C storage. Soil Biol. Biochem. 27(6):753-760 https://doi.org/10.1016/0038-0717(94)00242-S
  29. Klose, S. and M.A. Tabatabai. 1999. Urease activity of microbial biomass in soils. Soil Biol. Biochem. 31:205-211 https://doi.org/10.1016/S0038-0717(98)00090-X
  30. Klose, S. and M.A.Tabatabai. 2000. Urease activity of microbial biomass in soils as affected by cropping systems. Biol. Fertil. Soils. 31:191-199 https://doi.org/10.1007/s003740050645
  31. Knorr, W., I.C. Prentice, J.I. House, and E.A. Holland. 2005. Longterm sensitivity of soil carbon turnover to warming. Nature. 433 298-301 https://doi.org/10.1038/nature03226
  32. Koch, O., D. Tscherko, and E. Kandeler. 2007. Temperature sensitivity of microbial respiration, nitrogen mineralization, and potential soil enzyme activities in organic alpine soils. Global Biogeochem. Cycles. Japan. 21(4):GB4017 https://doi.org/10.1029/2007GB002983
  33. Lagomarsino, A., M.C. Moscatelli, A. D. Tizio, R. Mancinelli, S.Grego, S. Marinari. 2009. Soil biochemical indicators as a tool to assess the short-term impact of agricultural management on changes in organic C in a Mediterranean environment. Ecological indicators. 9:518-527 https://doi.org/10.1016/j.ecolind.2008.07.003
  34. Manzoni, S., A. Porpotato. 2007. A theoretical analysis of nonlinearities and feedbacks in soil arbon and nitrogen cycles. Soil Biol. Biochem. 39:1542-1556 https://doi.org/10.1016/j.soilbio.2007.01.006
  35. Marschner, P., E. Kandeler, and B. Marschner. 2003. Structure and function of the soil microbial community in a long-term fertilizer experiment. Soil Biol. Biochem. 35:453-461 https://doi.org/10.1016/S0038-0717(02)00297-3
  36. Mondini, C., M.L. Cayuela, T. Sinicco, M.A.S. Monedero, E. Bertolone, and L. Bardi. 2008. Soil application of meat and bone meal. short-term effects on mineralization dynamics an soil biochemical and microbiological properties. Soil Biol. Biochem.40:462-474 https://doi.org/10.1016/j.soilbio.2007.09.010
  37. Nanzyo, M. 2002. Unique properties of volcanic ash soils. Glob. Environ. Res. 6(2):99-112
  38. Nishiyama, M., Y. Sumikawa, G. Guan, and T. Marumoto. 2001. Relationship between microbial biomass and extractable organic carbon content in volcanic and non-volcanic ash soil. Appl. soil Ecol. 17(2):183-187 https://doi.org/10.1016/S0929-1393(01)00131-7
  39. Nobili, D.M., M. Contin, and P.C. Brookes. 2006.Microbial biomass dynamics in recently air-dried and rewetted soils compared to others stored air-dry for up to 103 years. Soil Biol. Biochem.38:2871-2881 https://doi.org/10.1016/j.soilbio.2006.04.044
  40. Paul, E.A. and F.E. Clark. 1989. Soil microbiology and biochemistry. Academic press, San Diego, CA
  41. Perucci, P. 1990. Effect of the addition of municipal solidwaste compost on microbial biomass and enzyme activities in soil.Biol Fertil Soils. 10:221-226
  42. RDA. 1988. Methods for chemical analysis of soil. Institute of Agricultural Technology
  43. Roscoe, R., C.A. Vasconcellos, A.E.F. Neto, G.A.A. Guedes, and L.A. Fernandes. 2000.Urease activity and its relation to soil organic matter, microbial biomass nitrogen and urea-nitrogen assimilation by maize in a Brazilian Oxisol under no-tillage and tillage systems.Biol Fertil Soils. 32:52-59 https://doi.org/10.1007/s003740000213
  44. Saito, M. 1990. Nitrogen mineralization parameters and its availability indices of soils in Tohoku district, Japan: Their relationship. Jpn. J. Soil Sci. Plant Nutr. 61(3):265-272
  45. Sardans, J., J. Penuelas, and M. Estiarte. 2008. Changes in soil enzymes related to C and N cycle and in soil C and N content under prolonged warming and drought in a Mediterranean shrubland. Appl. soil ecol. 39:223-235 https://doi.org/10.1016/j.apsoil.2007.12.011
  46. Satti, P., M.J. Mazzarino, L. Roselli, and P. Crego. 2007. Factors affection soil P dynamics in temperate volcanic soils of southern argentina. Geoderma. 139:229-240 https://doi.org/10.1016/j.geoderma.2007.02.005
  47. Sharifi, M., B.J. Zebarth, D.L. Burton, C.A. Grant, G.A. Porter. 2008. Organic amendment history and crop rotation effects on soil nitrogen mineralization potential and soil nitrogen supply in a potato cropping system. Agron. J. 100:1562-1572 https://doi.org/10.2134/agronj2008.0053
  48. Song, K. C. 1990. Andic properties of major soils in Cheju island.Ph. D. Thesis. Seoul National University. Suwon, Korea
  49. Stanford, G. and S.J. Smith. 1972. Nitrogen mineralization potentials of soils. Am. J. Soil Sci. Soc. 36:465-472 https://doi.org/10.2136/sssaj1972.03615995003600030029x
  50. Takenaka, M and K. Hayano. 1999. Investigation on the influence of the global warming on the mineralization of soil organic matter.Rese. Out. Agriculture, Forestry Fishery Tech. Bureau. 339:232-236
  51. Timothy, R.K., and R.P. Dick. 2004. Differentiating microbial and stabilized -glucosidase activity relative to soil quality. Soil Biol.Biochem. 36:2089-2096 https://doi.org/10.1016/j.soilbio.2004.06.007
  52. Tokashiki T. and K.Wada. 1975. Weathering implications of the mineralogy of clay fractions of two Ando soils. Kyushu.Geoderma. 14:47-62 https://doi.org/10.1016/0016-7061(75)90012-9
  53. Ugolini, F.C. and R.A. Dahlgren. 2002. Soil development in volcanic ash. Glob. Environ. Res. 6(2):69-81
  54. Vance, E.D., P.C. Brookes, and D.S. Jenkinson. 1987. Microbial biomass measurements in forest soils : the use of the chlroroform fumigation incubation method in strongly acid soils. Soil Biol.Biochem. 19:697-702 https://doi.org/10.1016/0038-0717(87)90051-4