Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Deciphering Functions of Uncultured Microorganisms

난배양성 미생물의 기능 분석 방법

  • Published : 2009.03.31
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Abstract

Microbes within complex communities show quite different physiology from pure cultured microbes. However, historically the study of microbes has focused on single species in pure culture and most of microbes are unculturable in our labs, so understanding of complex communities lags behind understanding of pure cultured cells. Methodologies including stable isotope probing (SIP), a combination of fluorescence in situ hybridization (FISH) and microautoradiography (MAR), isotope micrarray, and metagenomics have given insights into the uncultivated majority to link phylogenetic and functional information. Here, we review some of the most recent literatures, with an emphasis on methodological improvements to the sensitivity and utilities of these methods to link phylogeny and function in complex microbial communities.

미생물 군집 내의 미생물은 순수하게 배양된 미생물과는 다른 생리적 특징을 갖는다. 전통적으로 미생물 연구는 순수배양에 초점을 맞추어 이루어져 왔고 실제 생태계에 존재하는 대부분의 미생물들이 난배양성 미생물로 알려져 있다. 따라서 복잡한 미생물 군집에서 미생물의 기능에 대한 연구는 실질적으로 미진한 실정이다. 그러나 stable isotope probing (SIP), fluorescence in situ hybridization (FISH)와 microautoradiography (MAR)의 조합, isotope micrarray, 메타게노믹스 등의 새로운 분석방법들은 미생물 군집 내에서 난배양성 미생물의 기능 분석을 어느 정도 가능하게 해 주었다. 본 논문에서는 이들 방법 등에 대해 간단히 설명하고 좀 더 정확한 결과를 얻기 위한 최신 연구 동향을 소개하고자 한다.

Keywords

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