Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Identification and Cultural Characterization of Lipase Production Bacteria Isolated from Pond Effluent Sedimentary Layer

양식장 배출수 퇴적층에서 분리된 리파아제 생산 박테리아의 동정 및 배양학적 특성

  • Published : 2009.03.31
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Abstract

From the course of screening of useful enzyme producing microorganism from marine sedimentary layer, we isolated 2 lipase producing strains and their lipase producing activities were tested. 16S rDNA sequence analysis showed that they were Gram-positive bacteria grouped on Janibacter sp. An excellent lipase producing strain, Janibacter sp. LI-68 and J. sp. LI-80 identified by 16S rDNA analysis and biochemical methods (BIOLOG), was further studied its lipase producing characteristics. The optimum initial pH, temperature and the optimum cultral time for the enzyme production on MA medium were 8, $30{\sim}40^{\circ}C$ and 96 h, respectively.

제주 연안 양식장 배출수 퇴적층으로부터 미생물 배양 배지를 사용하여 총 200여 균주를 분리하였으며, 분리된 균주를 이용하여 olive oil이 함유된 평판배지에서 투명환을 형성하는 균주를 분리하였고, 이를 LI-68, LI-80로 각각 명명하였다. 분리 균주 LI-68, LI-80의 BIOLOG를 이용한 생화학적 분석 특성 및 16S rDNA의 염기서열 분석 결과 Janibacter anophelis와 99%의 유전적 상동성을 보여 최종적으로 Janibacter sp. LI-68, Janibacter sp. LI-80으로 동정되었다. 분리 균주 Janibacter sp. LI-68과 Janibacter sp. LI-80의 증식을 위한 최적 배양온도를 확인하고 증식온도에 따른 지방분해효소 활성의 변화를 조사하였으며, 이를 위하여 배양 온도를 $20^{\circ}C$에서 $40^{\circ}C$까지 $10^{\circ}C$ 간격으로 배양하여 균체의 증식과 분비되는 지질분해 효소의 활성을 조사하였다. 그 결과 분리균주 Janibacter sp. LI-68는 $30^{\circ}C$ 배양실험구에서 가장 높은 균 생육도를 나타냈으며, 효소활성은 균 생육도와는 다른 $40^{\circ}C$에서 가장 높은 효소활성을 보였다. 반면 Janibacter sp. LI-80 균주는 $30^{\circ}C$에서 가장 높은 균 생육도를 보였으며, 효소활성은 균 생육도와 반비례하여 $40^{\circ}C$에서 가장 높은 효소활성을 보였다.

Keywords

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