Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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A Tyrosinase Inhibitor Isolated from the Seeds of Eriobotrya japonica

비파 씨로부터 Tyrosinase 저해 활성물질의 분리

  • Kim, Tae-Hoon (Department of Herbal Medicinal Pharmacology, Daegu Haany University) ;
  • Shin, Seung-Ryeul (Faculty of Herbal Food & Nutrition, Daegu Haany University) ;
  • Kim, Tae-Wan (Faculty of Food Science & Biotechnology, Andong National University) ;
  • Lee, In-Chul (Senior Industry Cluster Agency, Youngdong University) ;
  • Park, Moon-Young (Subtropical Fruit Crops Experiment Station, Jeonnam Agricultural Research & Extension Services) ;
  • Jo, Cheo-Run (Department of Animal Science and Biotechnology, Chungnam National University)
  • 김태훈 (대구한의대학교 한약재약리학과) ;
  • 신승렬 (대구한의대학교 한방식품조리영양학부) ;
  • 김태완 (안동대학교 생명자원과학부) ;
  • 이인철 (영동대학교 고령친화산업 기업지원센터) ;
  • 박문영 (전남농업기술원 난지과수시험장) ;
  • 조철훈 (충남대학교 동물자원생명과학과)
  • Published : 2009.06.30
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Abstract

Activity-guided isolation from the ethylacetate (EtOAc)-soluble portion of a methanolic extract of the seeds of Eriobotrya japonica, using several bioassays, led to the isolation and identification of six phenolic compounds of previously known structure: benzaldehyde (1), chlorogenic acid (2), caffeic acid (3), benzoic acid (4), ferulic acid (5), and amygdalin (6). Of these, benzaldehyde (1) exhibited tyrosinase inhibitory activity in a bioassay. In addition, chlorogenic acid (2) and caffeic acid (3) were found to have strong antioxidative effects on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and superoxide dismutase (SOD)-like activity.

비파 씨를 MeOH로 침지 추출하여 얻어진 추출물을 n-hexane, EtOAc, n-BuOH 및 $H_2O$로 용매 분획하였다. 이중 tyrosinase, DPPH 및 SOD 활성이 상대적으로 높은 EtOAc 분획에 대해 column chromatography를 이용하여 6개의 페놀성 화합물을 분리하였다. 각 화합물의 화학구조는 NMR 스펙트럼 데이터 해석 및 표품과의 HPLC 직접 비교를 통하여 benzaldehyde (1), chlorogenic acid (2), caffeic acid (3), benzoic acid (4), ferulic acid (5) 및 amygdalin (6)으로 동정하였다. 이들 화합물중 benzaldehyde (1)는 125 mg/mL농도에서 tyrosinase저해활성이 70.8%로 나타나 positive control인 kojic acid의 56.3%보다 높게 나타났다. 또한 분리한 화합물 중 DPPH 라디칼 소거능은 12.5 mg/mL 실험농도에서 chlorogenic acid (2)와 caffeic acid (3)가 각각 43.7, 81.5%의 저해활성을 나타내었으며, 비파 씨의 SOD 활성물질은 chlorogenic acid (2)임을 확인하였다. 향후 이들 활성물질의 활성 기작에 대한 연구가 필요하며 본 연구결과는 보다 안전하고 우수한 tyrosinase 및 라디칼소거능을 가지는 새로운 선도화합물 발굴을 위한 기초자료로 이용될 수 있을뿐만 아니라 비파 씨의 식물 화학적 성분에 대한 기초자료로 이용될 수 있을 것으로 사료된다.

Keywords

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