Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
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Investigation of Microbial Contamination of Public Bath in Jongno-gu, Seoul

서울특별시 종로구 대중목욕탕의 수질 중 미생물 오염도 조사 연구

  • Kim, Mi-Soon (Department of Environmental Health, School of Public Health, Seoul National University) ;
  • Lee, Young-Min (Department of Environmental Health, School of Public Health, Seoul National University) ;
  • Kim, Seong-Keun (Department of Environmental Health, School of Public Health, Seoul National University) ;
  • Seo, Ji-Hyun (Department of Environmental Health, School of Public Health, Seoul National University) ;
  • Ji, Kyung-Hee (Department of Environmental Health, School of Public Health, Seoul National University) ;
  • Oh, Ji-Yoon (Department of Environmental Health, School of Public Health, Seoul National University) ;
  • Ko, Ki-Dong (Department of Environmental Health, School of Public Health, Seoul National University) ;
  • Ko, Gwang-Pyo (Department of Environmental Health, School of Public Health, Seoul National University)
  • 김미순 (서울대학교 보건대학원 환경보건학과) ;
  • 이영민 (서울대학교 보건대학원 환경보건학과) ;
  • 김성근 (서울대학교 보건대학원 환경보건학과) ;
  • 서지현 (서울대학교 보건대학원 환경보건학과) ;
  • 지경희 (서울대학교 보건대학원 환경보건학과) ;
  • 오지윤 (서울대학교 보건대학원 환경보건학과) ;
  • 고기동 (서울대학교 보건대학원 환경보건학과) ;
  • 고광표 (서울대학교 보건대학원 환경보건학과)
  • Published : 2009.06.30
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Abstract

The aim of this study is to investigate microbial sanitary condition of public baths in Seoul, Korea. A total of 28 water samples were collected from 14 different public baths and sudatoriums. The prevalence of fecal indicator microorganisms such as total coliform, fecal coliform, and Escherichia coli was characterized. In addition, bacteria in water was membrane filtered by 0.45um nitrocellulose membrane, and the filter was analyzed by both cultivation and PCR amplification of partial 16S rRNA gene. The levels of chlorine were measured for each of water samples. More than 40% of 14 collected water samples, the concentrations of total coliform bacteria exceeded the water quality for bath water guideline. There was no significant correlation between chlorine residue and the presence of total coliform. Various microorganisms including pathogenic microorganisms were identified from cultivation and subsequent analysis of 16s rRNA gene sequences. Our results suggest that appropriate hygiene practice and continuous monitoring is needed for reducing health risk associated with public bathhouses.

Keywords

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  2. Occurrence of Indicator Bacteria and Identification of Total Coliforms Using 16S rRNA Gene in Drinking Spring Water in Seoul vol.39, pp.6, 2013, https://doi.org/10.5668/JEHS.2013.39.6.513