한국자기공명학회논문지 (Journal of the Korean Magnetic Resonance Society)

한국자기공명학회 (Korean Magnetic Resonance Society)
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NMR characterization of SRG3 SWIRM Domain Mutant Proteins.

  • Koh, Woo-Hyoung (Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University) ;
  • Kim, Min-Tae (Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University) ;
  • Moon, Sun-Jin (Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University) ;
  • Lee, Weon-Tae (Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University)
  • 발행 : 2009.06.20
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초록

SWIRM domain, a core domain of SRG3 is well conserved in SW13, RSC8, and MOIRA family proteins. To understand structural basis for cellular functions of the SWIRM domain, we have initiated biochemical and structural studies on SWIRM domain and mutants using gelfiltration chromatography, circular dichroism and NMR spectroscopy. The structural properties of the mutant SWIRM domains (K34A and M75A) have been characterized, showing that the structures of both wild-type and mutant proteins are a-helical conformation. The data conclude that mutations at interaction sites of its binding partner protein do not affect its secondary and tertiary structure.

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참고문헌

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