Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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The Geographical Discrimination of Korean and Chinese Soybeans (Glycine max(L.) merrill) Using NMR Relaxation Methods

NMR relaxation 기법을 이용한 한국산과 중국산 대두의 원산지 판별

  • Published : 2009.06.30
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Abstract

To discriminate the geographical origin (Korea vs. China) of soybean (Glycine max(L.) merrill) samples (Korean samples n=25, Chinese samples n=24), proximate composition of soybeans and relaxation times were analyzed using low field NMR. Composition results indicate that there are no significant differences in moisture, fat, or ash contents between soybeans. The crude protein content of Korean soybeans, however, was higher than that of Chinese soybeans (p<0.05). The relaxation times of T1-IR (p<0.0001), T1-SR (p<0.0001), and T2-SE (p<0.0086) in Korean soybeans were longer than those in Chinese soybeans. The geographical origin of soybeans could be identified using a canonical discriminant analysis using two relaxation times (T1-IR and T1-SR) with 96% accuracy. Furthermore, in this study, a canonical discriminant analysis using four relaxation times (T1-IR, T1-SR, T2-SE, and T2-CPMG) could discriminate the geographical origin with 100% accuracy. It was possible to identify the geographical origin of Korean and Chinese soybeans using relaxation times from 10 MHz NMR.

국내산 콩 25종과 중국산 콩 24종을 일반성분 분석을 실시하고 10MHz pulsed NMR의 relaxation pattern을 이용하여 농산물의 원산지에 따른 차이를 구명하고 이에 따른 정확하고 신속한 원산지 판별이 가능하도록 하였다. 국내산과 중국산 콩의 일반성분 분석을 실시한 결과 수분, 지방, 회분에 유의적 차이가 없었고 조단백질 함량에서는 국내산이 중국산보다 높았다. T1-IR을 이용하여 측정한 NMR값은 중국산 콩이 국내산 콩보다 짧은 이완시간을 갖는 것으로 나타났으며(p<0.0001), $T_1-Sr$도 마찬가지였다(p<0.0001). 횡이완시간 중 $T_2-SE$의 값은 국내산이 중국산보다 높게 나타났다(t-test, p<0.0086). $T_1-IR$과 $T_1-SR$ 두 개의 변수를 이용하여 판별식을 작성한 경우 국내산과 중국산 모두 96%의 판별 가능성을 보였으며, $T_1-IR$과 $T_1-SR$, $T_2-SE$, $T_2-CPMG$의 모든 변수를 이용하여 판별한 결과 국내산과 중국산 콩의 판별 정확성이 100%로 나타났다. 이 연구를 통하여 저자장 NMR을 이용하여 국내산과 중국산 콩을 유의적 선별해 낼 수 있는 가능성을 확인하였다.

Keywords

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