한국식품과학회지 (Korean Journal of Food Science and Technology)

한국식품과학회 (Korean Society of Food Science and Technology)
권호 표지

밤가공품 중 polycyclic aromatic hydrocarbons 함량 분석

Determination of Polycyclic Aromatic Hydrocarbons in Processed Chestnut Products

  • 서일원 (동국대학교 식품공학과 및 Lotus기능성식품소재연구소) ;
  • 남혜정 (동국대학교 식품공학과 및 Lotus기능성식품소재연구소) ;
  • 김동혁 (동국대학교 식품공학과 및 Lotus기능성식품소재연구소) ;
  • 신한승 (동국대학교 식품공학과 및 Lotus기능성식품소재연구소)
  • Seo, Il-Won (Department of food Science and Technology and Institute of Lotus Functional Food Ingredient, Dongguk University) ;
  • Nam, He-Jung (Department of food Science and Technology and Institute of Lotus Functional Food Ingredient, Dongguk University) ;
  • Kim, Dong-Hyuk (Department of food Science and Technology and Institute of Lotus Functional Food Ingredient, Dongguk University) ;
  • Shin, Han-Seung (Department of food Science and Technology and Institute of Lotus Functional Food Ingredient, Dongguk University)
  • 발행 : 2009.06.30
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초록

본 실험은 밤가공품의 제조과정 중에 생성될 수 있는 PAHs 함량을 분석하기 위해 실시되었다. 밤가공품은 시중에서 유통되고 있는 7종을 구입하여 초음파 추출과정과 정제과정을 거친 후 HPLC/FLD를 이용하여 분석하였다. 분석 결과 8종류의 PAH는정성 정량분석에 만족할만한 다음의 결과를 얻었다. 첫째 PAHs를 농도범위에서 측정한 결과 상관계수($R_2$)가 0.997이상으로 분석에 양호한 직선성을 나타냈으며, 검출한계는 0.012-0.382 ${mu}g/kg$, 정량한계는 0.042-1.273 ${mu}g/kg$으로 만족할 만한 수준이었다. 둘째 PAHs와 공시료를 대상으로 회수율을 측정한 결과 평균회수율은 87.83-100.56%이고, 상대표준편차는 0.60-3.76%로 나타났다. 셋째 일내, 일간 정확성은 각각 81.95-125.44%, 79.89-116.53%이었고, 정밀성은 0.02-4.48%, 0.37-9.83%이었다. 넷째, 밤가공품을 대상으로 분석한 결과 PAHs 중 일부는 검출되었으나 모두 정량한계 이하로 나타났고, 대부분의 시료에서는 검출되지 않았다.

Concentrations of polycyclic aromatic hydrocarbon (PAH) in processed chestnut products were determined by HPLC/FLD. The methodology involved procedures of sonication with water, extraction with hexane, and clean-up on a Sep-pak florisil cartridge. The PAH limits of detection (LOD) and limits of quantitation (LOQ) ranged from 0.012 to 0.382 ${mu}g/kg$ and from 0.042 to 1.273 ${mu}g/kg$, respectively. The coefficients of variation for intra- and inter-day assays were 0.02-4.48% and 0.37-9.83%, respectively, and the accuracies were 81.95-125.44% and 79.89-116.53%, respectively. The overall recoveries for eight PAHs spiked into the processed chestnut products ranged from 87.83 to 100.56%. As a result, PAH contents were not detected in the processed chestnut products.

키워드

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