Journal of Oriental Neuropsychiatry (동의신경정신과학회지)

The Korean Society of Oriental Neuropsychiatry (대한한방신경정신과학회)
권호 표지

The Antioxidative and Neuroprotective Effect of Guibi-tang(Guipitang) and Guibi-tang gamibang(Guipitang jiaweijang) on PC12 cells.

귀비탕(歸脾湯)과 귀비탕가미방(歸脾湯加味方)의 항산화 효과 및 6-Hydroxydopamine에 대한 PC12 세포 보호효과 비교연구

  • Lim, Jae-Whan (Department of Neuropsychiatry, College of Oriental Medicine, Kyung-Hee University) ;
  • Kim, Jong-Woo (Department of Neuropsychiatry, College of Oriental Medicine, Kyung-Hee University) ;
  • Chung, Sun-Yong (Department of Neuropsychiatry, College of Oriental Medicine, Kyung-Hee University) ;
  • Cho, Sung-Hoon (Department of Neuropsychiatry, College of Oriental Medicine, Kyung-Hee University) ;
  • Oh, Myung-Sook (Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung-Hee University) ;
  • Hwang, Wei-Wan (Department of Neuropsychiatry, College of Oriental Medicine, Kyung-Hee University)
  • 임재환 (경희대학교 한의과대학 한방신경정신과 교실) ;
  • 김종우 (경희대학교 한의과대학 한방신경정신과 교실) ;
  • 정선용 (경희대학교 한의과대학 한방신경정신과 교실) ;
  • 조성훈 (경희대학교 한의과대학 한방신경정신과 교실) ;
  • 오명숙 (경희대학교 약학대학 한약학과) ;
  • 황의완 (경희대학교 한의과대학 한방신경정신과 교실)
  • Published : 2009.03.30
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Abstract

Objectives : This Study was performed to assess the antioxidative and neuroprotective effect of Guibi-tang(Guipi-tang) and Guibi-tang gamibang(Guipitang jiaweifang) on PC12 cells. Methods : The antioxidative effect was investigated through the DPPH radical and ABTS cation scavenging methods and total polyphenol amount of Guibi-tang(Guipitang) and Guibi-tang gamibang(Guipitang jiaweifang). The neuroprotective effect of Guibi-tang(Guipitang) and Guibi-tang gamibang(Guipitang jiaweifang) was assessed using MTT assay in PC12 cells. The scavenging effect of Guibi-tang(Guipitang) and Guibi-tang gamibang(Guipitang jiaweifang) on NO and ROS production induced by 6-OHDA in PC12 cells was evaluated, as well as the attenuating effect of Guibi-tang gamibang(Guipitang jiaweifang) on GSH reduction. Results : 1. Guibi-tang(Guipitang) and Guibi-tang gamibang(Guipitang jiaweifang) had concentration-dependent scavenging activities of DPPH radical 2. Guibi-tang(Guipitang) and Guibi-tang gamibang(Guipitang jiaweifang) had concentration-dependent scavenging activities of ABTS cation. 3. Total polyphenol amount of Guibi-tang(Guipitang) and Guibi-tang gamibang(Guipitang jiaweifang) was calculated 79.10${\pm}$2.20 pg/IO mg and 121.03${\pm}$1.11 pg/IO mg, respectively. 4. Cell viability of Guibi-tang(Guipitang) was increased in a dose dependent manner. Guibi-tang gamibang(Guipitang jiaweifang) was increased at low concentrations, but decreased at high concentrations. 5. In Guibi-tang(Guipitang), cell viability of PC12 cell treated with 6-OHDA was decreased by pre-treatment, and increased by post- and co- treatment. Cell viability of Guibi-tang gamibang(Guipitang jiaweifang) showed variable effects by pre-treatment, but increased by post- and co- treatment. 6. NO production rate of Guibi-tang(Guipitang) didn't show a significant effect, but that of Guibi-tang gamibang(Guipitang jiaweifang) was decreased in a dose dependent manner. 7. ROS production rate of Guibi-tang(Guipitang) was decreased at some concentrations. In Guibi-tang gamibang(Guipitang jiaweifang), ROS production rate was decreased at high concentrations. 8. Guibi-tang gamibang(Guipitang jiaweifang) protected the 6-OHDA-induced GSH reduction. Conclusions : These results demonstrate that both Guibi-tang(Guipitang) and GBTGMB have antioxidative and neuroprotective effect, but Guibi-tang gamibang(Guipitang jiaweifang) has more antioxidative and neuroprotective effect than Guibi-tang.

Keywords

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