Journal of Society of Preventive Korean Medicine (대한예방한의학회지)

Society of Preventive Korean Medicine (대한예방한의학회)
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Cytoprotective Effect of Phenolic Compounds Against Hexavalent Chromium-Induced Cytotoxicity

페놀 화합물의 Hexavalent Chromium독성에 대한 세포 보호효과

  • Han, Du-Seok (Department of Oral Anatomy, School of Dentistry, Wonkwang University) ;
  • Kang, Jeong-Il (Department of Physical Therapy, Daebul University) ;
  • Baek, Seung-Hwa (Department of Herbal Resources, Professional School of Oriental Medicine, Wonkwang University)
  • 한두석 (원광대학교 치과대학 구강해부학교실) ;
  • 강정일 (대불대학교 물리치료학과) ;
  • 백승화 (원광대학교 한의학전문대학원 한약자원개발학과)
  • Received : 2009.05.27
  • Accepted : 2009.07.22
  • Published : 2009.08.30
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Abstract

Objectives : In order to evaluate the cytotoxicity of hexavalent chromium, the cytoprotective effect of phenolic compounds against hexavalent chromium-induced cytotoxicity, cell viability, cell adhesion ability, lactate dehydrogenase(LDH) activity, and morphological changes of cells were examined. Methods : We measured the cytotoxicity of hexavalent chromium with 3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyltetrazolium bromide(MTT), 2,3-bis-[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-caboxanilide (XTT), LDH and DPPH methods. Results : The cytotoxicity of hexavalent chromium($IC_{50}$, $44.0-51.0{\mu}M$) was high according to the toxic criteria. Cytoprotective effect of phenolic compounds against $IC_{50}$ value of hexavalent chromium in cell morphology increased in a concentration-dependent manner. Conclusions : These results suggest that 3,4,5-trihydroxybenzoic acid may be used as a cytoprotective agent against chromium(IV)-mediated cytotoxicity.

Keywords

References

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