Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effect of Rosmarinus officinalis L. on Growth Inhibition and Apoptosis Induction in Cancer Cells

로즈마리(Rosmarinus offcinalis L.) 분획물의 암세포에 대한 성장억제 및 세포사멸 유도 효과

  • Choi, Jun-Hyeok (Dept. of Food Science and Technology, The Center for Traditional Microorganism Resources, Keimyung University) ;
  • Kim, Hyuk-Il (Dept. of Food Science and Technology, The Center for Traditional Microorganism Resources, Keimyung University) ;
  • Lee, In-Seon (Dept. of Food Science and Technology, The Center for Traditional Microorganism Resources, Keimyung University)
  • 최준혁 (계명대학교 식품가공학, 전통미생물 자원 개발 및 산업화 연구 센터) ;
  • 김혁일 (계명대학교 식품가공학, 전통미생물 자원 개발 및 산업화 연구 센터) ;
  • 이인선 (계명대학교 식품가공학, 전통미생물 자원 개발 및 산업화 연구 센터)
  • Published : 2009.08.31
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Abstract

The goal of this study was to evaluate the anticancer effect of Rosmarinus officinalis L. In this study induction of apoptosis by methanol extract of rosemary and their fractions were investigated in vitro. In examining the effect of rosemary methanol extract on the inhibition of growth of Hela, HepG2, A549, AGS cells and HT-29 cell, it was found that the methanol extract of rosemary and their fractions demonstrated a cytotoxic effect in a dose-dependent manner; in addition, hexane and chloroform fractions showed a particularly high cytotoxic effect on Hela and AGS cells. The results showed that the hexane and chloroform fractions of rosemary have cytotoxic effect which are related to the activity of the essential oil in the rosemary. Apoptosis in Hela and AGS cells mediated by the hexane and chloroform fractions was associated with the increase of cleaved caspase-3 levels and cleaved PARP. Therefore, with more researches on identification and action mechanism of active compound, the hexane and chloroform fractions are expected to be natural sources for the developments of functional food and medical agents to prevent gastric cancer and uterus cancer.

최근 다방면에서 효능이 알려지고 있는 로즈마리를 이용하여 apoptosis 유도기전을 해석하기 위하여 인간유래 자궁암 Hela 세포주를 대상으로 조사한 결과는 다음과 같다. 로즈마리 분획물 중 hexane층과 chloroform층의 처리 농도 의존적으로 Hela 자궁암세포의 저해활성은 현저하게 감소되었으며 이는 apoptosis 유도와 연관성이 있었다. 로즈마리 분획물 중 hexane층과 chloroform층의 처리에 의하여 apoptosis 억제에 관여하는 Bcl-2 단백질의 발현은 감소되었으며, apoptosis 유도에 관여하는 Bax 유전자의 발현은 농도 의존적으로 증가되었다. 로즈마리 분획물 중 hexane층과 chloroform층의 처리에 의한 apoptosis 유도는 caspase-3, 7, 9 및 PARP의 활성화와 연관성이 있었다.

Keywords

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