Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Changes of Saponin and β-Glucan Content on the Cultured Ginseng with Mushroom Mycelia

버섯균사체로 배양된 인삼 Saponin과 β-Glucan 함량 변화

  • Joung, Eun-Mi (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Hwang, In-Guk (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Lee, Hyeon-Yong (Dept. of Biomaterials Engineering, Kangwon National University) ;
  • Jeong, Jae-Hyun (Dept. of Food and Biotechnology, Chungju National University) ;
  • Yu, Kwang-Won (Dept. of Food and Biotechnology, Chungju National University) ;
  • Jeong, Heon-Sang (Dept. of Food Science and Technology, Chungbuk National University)
  • 정은미 (충북대학교 식품공학과) ;
  • 황인국 (충북대학교 식품공학과) ;
  • 이현용 (강원대학교 BT특성화학부대학 생물소재공학과) ;
  • 정재현 (충주대학교 식품생명공학부) ;
  • 유광원 (충주대학교 식품생명공학부) ;
  • 정헌상 (충북대학교 식품공학과)
  • Published : 2009.08.31
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Abstract

This study investigated the changes of saponin and $\beta$-glucan content on the cultured ginseng with mushroom mycelia of Phellinus linteus (PL), Ganoderma lucidum (GL), and Hericium erinaceum (HE). Cultured ginsengs with mushroom mycelia were extracted with 80% ethanol, fractionated with n-buthanol, and analysed for ginsenosides by high performance liquid chromatography (HPLC). Crude saponin content of raw ginseng was 4.11% (d.b) but cultured ginseng with mushroom mycelia of PL, GL, and HE were increased to 6.74, 6.77 and 6.23% (d.b), respectively. Ginsenoside-Rd, among the 12 ginsenosides which were available for analysis, was remarkably increased to 13.61, 24.26, and 32.69 mg/g, respectively (raw ginseng: 0.80 mg/g). The $\beta$-glucan content of cultured ginseng with mushroom mycelia of PL, GL, and HE were decreased to 8.85, 5.51 and 5.46% rather than mushroom mycelia of 29.14, 19.44, and 23.39% (d.b), respectively.

인삼을 배지로 사용하여 목질진흙버섯(Phellinus linteus), 영지버섯(Ganoderma lucidum) 및 노루궁뎅이버섯(Hericium erinaceum) 균사체를 접종 배양하여 얻어진 균사체 인삼배양물과 원료 인삼에 대한 사포닌과 $\beta$-glucan의 변화를 분석하였다. 원료인삼의 구성사포닌 함량은 22.47mg/g이었으나 균사체로 배양된 인삼의 사포닌 함량은 목질 진흙버섯이 39.78 mg/g, 영지버섯이 55.88 mg/g 그리고 노루궁뎅이버섯이 32.69 mg/g으로 원료인삼에 비해 증가하였다. 균사체 인삼배양물의 사포닌은 PPT계는 감소하였고 PPD계는 증가하였다. 구성사포닌 중 ginsenoside Rd는 원료인삼에 비해 균사체 인삼배양물에서 각각 17, 30 및 12배 증가하였다. 3종의 균사체와 균사체 인삼배양물들의 총 glucan 함량은 각각 46.24, 34.74 및 34.02% 그리고 28.77, 17.78 및 21.77%이었다. $\beta$-glucan 함량은 균사체에서는 각각 29.14, 19.44 및 23.39%이었고 인삼배양물에서는 각각 8.85, 5.46 및 5.51%로 나타나 목질진흙버섯이 균사체와 인삼배양물에서 가장 높은 함량을 나타내었다.

Keywords

References

  1. Ha DC, Ryu GH. 2005. Chemical components of red, white and extruded root ginseng. J Korean Soc Food Sci Nutr 34: 247-254 https://doi.org/10.3746/jkfn.2005.34.2.247
  2. Park CK, Jeon BS, Yang JW. 2003. The chemical components of Korean ginseng. Food Industry and Nutrition 8: 1226-3338
  3. Kitakawa I, Taniyama T, Yoshikawa M, Ikenishi Y, Nakagawa Y. 1989. Chemical structures of malonyleginsenosides Rb1, Rb2, Rc and Rd isolated from the root of Panax ginseng C.A. Meyer. Chem Pharm Bull 37: 2961-2970 https://doi.org/10.1248/cpb.37.2961
  4. Choi M, Shin GJ, Choi GP, Do JH, Kim JD. 2003. Synergistic effects of extracts from Korean red ginseng, Saururus chinensis (Lour.) Baill. and Rubus coreanus Miq. on antioxidative activities in rats. Korean J Medicinal Crop Sci 14: 27-30
  5. Lee SE, Lee SW, Bang JK, Yu YJ, Seong NS. 2001. Antioxidant activities of leaf, stem, and root of Panax ginseng C.A. Meyer. Korean J Medicinal Crop Sci 12: 237-242
  6. Lim HR, Shim MJ, Kim HW, Lee CO, Choi EC, Kim BK. 1984. Antitumor components extracted from the cultured mycelia of Lyophyllum decasstes. Korean J Phamacogn 15: 61-73
  7. Yanmaguchi M, Yearul KA. 1987. Effect of shitake and maitake mushroom on blood pressure and plasma lipids of spontaneously hypertensive rats. J Nutr Sci Vitaminol 33: 341-345 https://doi.org/10.3177/jnsv.33.341
  8. Park MH, Oh KY, Lee BW. 1998. Anti-cancer activity of Letinus edodes and Pleurotus astreatus. Korean J Food Sci Technol 30: 702-708
  9. Kim GJ, Kim HS, Chung SY. 1992. Effect of varied mushroom on lipid compositions in dietary hypercholestrolemic rats. J Korean Soc Food Nutr 21: 131-135
  10. Shon MY, Seo KI, Choi SY, Sung NJ, Lee SW, Park SK. 2006. Chemical compounds and biological activity of Phellinus baumii. J Korean Soc Food Sci Nutr 35: 524-529 https://doi.org/10.3746/jkfn.2006.35.5.524
  11. Kim JO, Jung MJ, Choi HJ, Lee JT, Lim AK, Hong JH, Kim DI. 2008. Antioxidative and biological activity of hot water and ethanol extracts from Phellinus linteus. J Korean Soc Food Sci Nutr 37: 684-690 https://doi.org/10.3746/jkfn.2008.37.6.684
  12. Atsumi S, Nosaka C, Ochi Y, Iinuma H, Umezawa K. 1993. Inhibition of experimental metastasis by an $\alpha$-glucosidase inhibitor, 1,6-epi-cyclophellitol. Cancer Res 53: 4896-4899
  13. Lee SY, Rhee HM. 1990. Cardiovascular effects of mycelium extract of Ganoderma lucidum; inhibition of sympathetic outflow as a mechanism of its hypotensive action. Chem Pharm Bull 38: 1359-1364 https://doi.org/10.1248/cpb.38.1359
  14. Adachi T, Ohno N, Ohsawa M, Oikawa S, Yadomae T. 1990. Macrophage activation in vitro by chemically crosslinked( 1$\rightarrow$3)-$\beta$-D-glucan. Chem Pharm Bull 38: 988-992 https://doi.org/10.1248/cpb.38.988
  15. Usui T, Iwasaki Y, Hayashi K, Mizuno T, Tanaka M, Shinkai K, Arakawa M. 1981. Antitumor activity of watersoluble $\beta$-D-glucan elaborated by Ganoderma applanatum. Agric Biol Chem 45: 323-326 https://doi.org/10.1271/bbb1961.45.323
  16. Sone Y, Okuda R, Wada N, Kishida E, Misaki A. 1985. Structures and antitumor activities of the polysaccharides isolated from fruiting body and the growing culture of mycelium of Ganoderma lucidum. Agric Biol Chem 49: 2641-2653 https://doi.org/10.1271/bbb1961.49.2641
  17. Kawagishi H, Shimada A, Hosokawa S, Mori H, Sakamono H, Ishiguro Y, Sakemi S, Bordner S, Kojima N, Furukawa S. 1996. Erinacines E, F, and G, stimulators of nerve growth factor (NGF)-synthesis, from the mycelia of Hericium erinaceum. Tetrahedron Lett 37: 7399-7402 https://doi.org/10.1016/0040-4039(96)01687-5
  18. Cho SM, Park JS, Kim KP, Cha DY, Kim HM, Yoo ID. 1999. Chemical features and purification of immunostimulating polysaccharides from the fruit bodies of Agaricus blazei . Korean J Mycology 27: 170-174
  19. Jeong JH, Wee JJ, Shin JY, Cho JH, Jung DH. 2005. Antioxidative effect of crude saponin fraction prepared from culture product of basidiomycota cultured with fresh ginseng as substrate. Korean J Food Sci Technol 37: 67-72
  20. Kim SK, Kwak YS, Kim SW, Hwang SY. 1998. Improved method for the preparation of crude ginseng saponin. J Ginseng Res 22: 155-160
  21. Sung JH, Hideo H, Satoshi M, Masamori U, Ha JY, Lee MS, Huh JD. 1995. Metabolism of ginseng saponins by human intestinal bacteria. Korean J Pharmacogn 26: 360-367
  22. Quan LH, Liang Z, Kim HB, Kim SH, Kim MK, Yang DC. 2008. Conversion of ginsenoside Rd to compound K by crude enzymes extracted from Lactobacillus brevis LH8. J Ginseng Res 32: 226-231 https://doi.org/10.5142/JGR.2008.32.3.226
  23. Quan LH, Cheng LQ, Na JR, Kim HB, Park MJ, Kim SH, Kim MK, Yang DC. 2008. Transformation of ginsenoside Rd to ginsenoside F2 by enzymes of Leuconostoc fallax LH3. Korean J Medicinal Crop Sci 16: 155-160
  24. Cheng LQ, Kim MK, Lee JW, Yang DC. 2006. Conversion of major ginsenoside $Rb_1$ to ginsenoside $F_2$ by Caulobactor leidyia. Biotechnol Lett 28: 1121-1127 https://doi.org/10.1007/s10529-006-9059-x
  25. Lee SH, Lim HM, Kim TY, Cho NS, Park JS, Ryu YW, Kim MS. 2004. Effective production of $\beta$-glucan by the liquid cultivation of Agaricus blazi . Korean J Microbiology 40: 54-59
  26. Manzi P, Pizzoferrato L. 2000. Beta-glucans in edible mushrooms. Food Chem 68: 315-318 https://doi.org/10.1016/S0308-8146(99)00197-1
  27. Hammond JBW. 1979. Changes in composition of harvested mushrooms (Agaricus bisporus). Phytochemistry 18: 415-418 https://doi.org/10.1016/S0031-9422(00)81878-6

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