Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Effect of Macromolecules in Maturation Medium on Oocyte Maturation and Embryonic Development after Parthenogenesis and Nuclear Transfer in Pigs

  • You, Jin-Young (School of Veterinary Medicine, Kangwon National University) ;
  • Kim, Jin-Young (School of Veterinary Medicine, Kangwon National University) ;
  • Lee, Eun-Song (School of Veterinary Medicine, Kangwon National University)
  • Published : 2009.06.30
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Abstract

The objective of this study was to examine the effect of macromolecule in a maturation medium on nuclear maturation, intracellular glutathione (GSH) level of oocytes, and embryonic development after parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) in pigs. Immature pig oocytes were cultured in maturation medium that was supplemented with each polyvinyl alcohol (PVA), pig follicular fluid (pFF) or newborn calf serum (NBCS) during the first 22 h and the second 22 h. Oocyte maturation was not influenced by the source of macromolecules during in vitro maturation (IVM). Embryo cleavage and cell number in blastocyst after PA was altered by the source of macromolecule but no difference was observed in blastocyst formation among treatments. Oocytes matured in PVA-PVA medium showed lower rates of oocyte-cell fusion (70.4% vs. 77${\sim}$82%) and embryo cleavage (75% vs. 86${\sim}$90%) after SCNT than those matured in other media but blastocyst formation was not altered (13${\sim}$27%) by different macromolecules. pFF added to IVM medium significantly increased the intracellular GSH level of oocytes compared to PVA and NBCS, particularly when pFF was supplemented during the first 22 h of IVM. Our results demonstrate that source of macromolecule in IVM medium influences developmental competence of oocytes after PA and SCNT, and that pFF supplementation during the early period (first 22 h) of IVM increases intracellular GSH level of oocytes.

Keywords

References

  1. Abeydeera LR, Wang WH, Prather RS and Day BN. 1998. Maturation in vitro of pig oocytes in protein-free culture media:fertilization and subsequent embryo development in vitro. BioI. Reprod. 58:1316-1320 https://doi.org/10.1095/biolreprod58.5.1316
  2. Bavister BD, Leibfried ML and Lieberman G. 1983. Development of preimplantation embryos of the golden hamster in a defined culture medium. BioI. Reprod. 28:235-247 https://doi.org/10.1095/biolreprod28.1.235
  3. Bing YZ, Hirao Y, Iga K, Che LM, Takenouchi N, Kuwayama M, Fuchimoto D, Rodriguez-Martinez H and Nagai T. 2002. In vitro maturation and glutathione synthesis of porcine oocytes in the presence or absence of cysteamine under different oxygen tensions: role of cumulus cells. Reprod. Fertil. Dev. 14:125-131 https://doi.org/10.1071/RD01127
  4. Carolan C, Lonergan P, Van Langendonckt A and Mermillod P. 1995. Factors affecting bovine embryo development in synthetic oviduct fluid following oocyte maturation and fertilization in vitro. Theriogenology 43:1115-1128 https://doi.org/10.1016/0093-691X(95)00075-J
  5. de Matos DG and Furnus CC. 2000. The importance of having high glutathione (GSH) level after bovine in vitro maturation on embryo development effect of $\beta$-mercaptoethanol, cysteine and cystine. Theriogenology 53:761-771 https://doi.org/10.1016/S0093-691X(99)00278-2
  6. Dostal J and Pavlok A. 1996. Isolation and characterization of maturation inhibiting compound in bovine follicular fluid. Reprod. Nutr. Dev. 36:681-690
  7. Funahashi H and Day BN. 1993. Effects of the duration of exposure to hormone supplements on cytoplasmic maturation of pig oocytes in vitro. J. Reprod. Fertil. 98:179-185 https://doi.org/10.1530/jrf.0.0980179
  8. Garcia-Mengual E, Alfonso J, Salvador I, Duque CC and Silvestre MA. 2008. Oocyte activation procedures and influence of serum on porcine oocyte maturation and subsequent parthenogenetic and nuclear transfer embryo development. Zygote 16:279-284 https://doi.org/10.1017/S0967199408004796
  9. Hong J and Lee E. 2007. Intrafollicular amino acid concentration and the effect of amino acids in a defined maturation medium on porcine oocytes maturation, fertilization, and preimplantation development. Theriogenology 68:728-735 https://doi.org/10.1016/j.theriogenology.2007.06.002
  10. Isobe N, Maeda T and Terada T. 1998. Involvement of meiotic resumption in the disruption of gap junctions between cumulus cells attached to pig oocytes. J. Reprod. Fertil. 113:167-172 https://doi.org/10.1530/jrf.0.1130167
  11. Jeong YW, Hossein MS, Bhandari DP, Kim YW, Kim JH, Park SW, Lee E, Park SM, Jeong YI, Lee JY, Kim S and Hwang WS. 2008. Effects of insulin-transferrin-selenium in defined and porcine follicular fluid supplemented IVM media on porcine IVF and SCNT embryo production. Anim. Reprod. Sci. 106:13-24 https://doi.org/10.1016/j.anireprosci.2007.03.021
  12. Kawakami M, Tani T, Yabuuchi A, Kobayashi T, Murakami H, Fujimura T, Kato Y and Tsunoda Y. 2003. Effect of demecolcine and nocodazole on the efficiency of chemically assisted removal of chromosomes and the developmental potential of nuclear transferred porcine oocytes. Cloning Stem Cells 5:379-387 https://doi.org/10.1089/153623003772032871
  13. Khatir H, Carolan C, Lonergan P and Mermillod P. 1997. Characterization of calf follicular fluid and its ability to support cytoplasmic maturation of cow and calf oocytes. J. Reprod. Fertil. 111:267-275 https://doi.org/10.1530/jrf.0.1110267
  14. Kobayashi M, Asakuma S and Fukui Y. 2007. Blastocyst production by in vitro maturation and development of porcine oocytes in defined media following intracytoplasmic sperm injection. Zygote 15:93-102 https://doi.org/10.1017/S0967199406004035
  15. Liu R-H, Li Y-H, Jiao L-H, Wang X-N, Wang H and Wang W-H. 2002. Extracellular and intracellular factors affecting nuclear and cytoplasmic maturation of porcine oocytes collected from different sizes of follicles. Zygote 10:253-260 https://doi.org/10.1017/S0967199402002332
  16. Mori T, Amano T and Shimizu H. 2000. Roles of gap junctional communication of cumulus cells in cytoplasmic maturation of porcine oocytes cultured in vitro. BioI. Reprod., 62:913-919 https://doi.org/10.1095/biolreprod62.4.913
  17. Motlik J, Fulka J and Flechon JE. 1986. Changes in intercellular coupling between pig oocytes and cumulus cells during maturation in vivo and in vitro. J. Reprod. FertiI. 76:31-37 https://doi.org/10.1530/jrf.0.0760031
  18. Orsi NM, Gopichandran N, Leese HJ, Picton HM and Harris SE. 2005. Fluctuations in bovine ovarian follicular fluid throughout the oestrous cycle: a comparison with plasma and a TCM-199-based maturation medium. Reproduction 129:229-334 https://doi.org/10.1530/rep.1.00336
  19. Polejaeva lA, Chen SH, Vaught TD, Page RL, Mullins J, Ball S, Dai Y, Boone J, Walker S, Ayares DL, Colman A and Campbell KH. 2000. Cloned pigs produced by nuclear transfer from adult somatic cells. Nature 407:86-90 https://doi.org/10.1038/35024082
  20. Rath D, Niemann H and Tao T. 1995. In vitro maturation of porcine oocytes in follicular fluid with subsequent effects on fertilization and embryo yield in vitro. Theriogenology 44:529-538 https://doi.org/10.1016/0093-691X(95)00224-V
  21. Sakatani M, Suda I, Oki T, Kobayashi S, Kobayashi S and Takahashi M. 2007. Effects of purple sweet potato anthocyanins on development and intracellular redox status of bovine preimplantation embryos exposed to heat shock. J. Reprod. Dev. 53:605-14 https://doi.org/10.1262/jrd.18124
  22. Schoevers EJ, Kidson A, Verheijden JH and Bevers MM. 2003. Effect of follicle-stimulating hormone on nuclear and cytoplasmic maturation of sow oocytes in vitro. Theriogenology 59:2017-2028 https://doi.org/10.1016/S0093-691X(02)01288-8
  23. Seli E, Zeyneloglu HB, Senturk LM, Bahtiyar OM, Olive DL and Arici A. 1998. Basic fibroblast growth factor: Peritoneal and follicular fluid levels and its effect on early embryonic development. Fertil. Steril. 69:1145-1148 https://doi.org/10.1016/S0015-0282(98)00074-0
  24. Song K and Lee E. 2007. Modification of maturation condition improves oocyte maturation and in vitro development of somatic cell nuclear transfer pig embryos. J. Vet. Sci. 8:81-87 https://doi.org/10.4142/jvs.2007.8.1.81
  25. Song K, Hyun SH, Shin T and Lee E. 2009. Post-activation treatment with demecolcine improves development of somatic cell nuclear transfer embryos in pigs by modifying the remodeling of donor nuclei. Mol. Reprod. Dev. 76:611-619 https://doi.org/10.1002/mrd.20989
  26. Takahashi M, Nagai T, Okamura N, Takahashi H and Okano A. 2002. Promoting effect of $\beta$-mercaptoethanol on in vitro development under oxidative stress and cystine uptake of bovine embryos. BioI. Reprod. 66:562-567 https://doi.org/10.1095/biolreprod66.3.562
  27. Walker SC, Shin T, Zaunbrecher GM, Romano JE, Johnson GA, Bazer FW and Piedrahita JA. 2002. A highly efficient method for porcine cloning by nuclear transfer using in vitro-matured oocytes, Cloning Stem Cells 4:105-112 https://doi.org/10.1089/153623002320253283
  28. Wiesak T, Hunter MG and Foxcroft GR. 1990. Differences in follicular morphology, steroidogenesis and oocyte maturation in naturally cyclic and PMSG/hCG-treated prepubertal gilts. J. Reprod. FertiI. 89:633-641 https://doi.org/10.1530/jrf.0.0890633
  29. Wilmut I, Schnieke AE, McWhir J, Kind AJ and Campbell KH. 1997. Viable offspring derived from fetal and adult mammalian cells. Nature 385:810-813 https://doi.org/10.1038/385810a0
  30. Yoshida M, Ishizaki Y, Kawagishi H, Bamba K and Kojima Y. 1992. Effects of pig follicular fluid on maturation of pig oocytes in vitro and on their subsequent fertilizing and developmental capacity in vitro. J. Reprod. Fertil. 95:481-488 https://doi.org/10.1530/jrf.0.0950481
  31. Yoshioka K, Suzuki C, Tanaka A, Anas IM and Iwamura S. 2002. Birth of piglets derived from porcine zygotes cultured in a chemically defined medium. BioI. Reprod. 66:112-119 https://doi.org/10.1095/biolreprod66.1.112