Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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Multiplex PCR Detection of 4 Events of Genetically Modified Soybeans (RRS, A2704-12, DP356043-5, and MON89788)

  • Kim, Jae-Hwan (Institute of Life Sciences and Resources and Department of Food Science & Biotechnology, Kyung Hee University) ;
  • Seo, Young-Ju (Institute of Life Sciences and Resources and Department of Food Science & Biotechnology, Kyung Hee University) ;
  • Sun, Seol-Hee (Institute of Life Sciences and Resources and Department of Food Science & Biotechnology, Kyung Hee University) ;
  • Kim, Hae-Yeong (Institute of Life Sciences and Resources and Department of Food Science & Biotechnology, Kyung Hee University)
  • Published : 2009.06.30
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Abstract

A multiplex polymerase chain reaction (PCR) method was developed for the detection of 4 events of genetically modified (GM) soybean. The event-specific primers were designed from 4 events of GM soybean (RRS, A2704-12, DP356043-5, and MON89788). The lectin was used as an endogenous reference gene of soybean in the PCR detection. The primer pair YjLec-4-F/R producing 100 bp amplicon was used to amplify the lectin gene and no amplified product was observed in any of the 9 different plants used as templates. This multiplex PCR method allowed for the detection of event-specific targets in a genomic DNA mixture of up to 1% GM soybean mixture containing RRS, A2704-12, DP356043-5, and MON89788. In this study, 20 soybean products obtained from commercial food markets were analyzed by the multiplex PCR. As a result, 6 samples contained RRS. These results indicate that this multiplex PCR method could be a useful tool for monitoring GM soybean.

Keywords

References

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