Experimental Study on Effects of Cucurbita moschata Duch. on Antioxidation

호박잎의 항산화 효과 연구

  • Cha, Yun-Yeop (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Sangji University)
  • 차윤엽 (상지대학교 한의과대학 한방재활의학교실)
  • Received : 2009.11.03
  • Accepted : 2009.11.19
  • Published : 2009.12.30

Abstract

In recent year, We are concerned about anti-aging, disease-protection, long-life, many methods are used in solving this problem. And Those are related with antioxidative ability. Recently, We heard that Cucurbita moschata Duch. has anti-hypertensive effect and good for health. So I let made a experiment for this result of the anti-oxidant effect of Cucurbita moschata Duch. used for 3 methods, those are DPPH radical scavenging activity, Nitric oxide(NO) radical scavenging activity and Bovine serum albumin(BSA). The results of this study were as follows: We measured level of DPPH radical scavenging activity. And we obtained results that the ability of DPPH radical's elimination was increased when concentration of Cucurbita moschata Duch. was to $20\;mg/m{\ell}$. We measured level of Nitric oxide(NO) radical scavenging activity. And we founded that the ability of NO radical's elimination was increased when concentration of Cucurbita moschata Duch. was to $10\;mg/m{\ell}$. When we inspected antioxidation with Bovine serum albumin(BSA). And we obtained that antioxidative ability was increased after$2.5\;mg/m{\ell}$ of Cucurbita moschata Duch.. So I guess that Cucurbita moschata Duch. has effect of antioxidative ability. Hereafter we need differential experimental methods for evidence of antioxidative effect on Cucurbita moschata Duch.

Keywords

References

  1. 육창주. 원색한국약용식물도감. 아카데미서적. 서울. 1990:584.
  2. 황도연. 증맥.방약합편. 남산당. 서울. 2000:260, 296, 300.
  3. 이시진. 본초강목. 인민위생출판사. 북경. 1975:1700, 2451.
  4. 김숙희, 김화영. 노화. 서울. 민음사. 77-85, 94. 1991.
  5. 李獻平 外. 四大懷藥延綬衰老作用的硏究. 서울. 中西醫結合雜誌. 11(8). 486-7. 1991.
  6. Cutler, R. G. Antioxidant, aging and longevity. Free Radicals in Biology(ed. Pryor, W.). Acedimic Press. (6). 371-424. 1984.
  7. 장석태. 피부과학. 서울. 여문각. 23-5. 1994.
  8. Kai-Jin Wang, Ying-Jun Zhang, Chong-Ren Yang. Antioxidant phenolic constituents from Fagopyrum dibortrys. Journal of Ethnopharmacology 99. 2005:259-64.
  9. Manjeshwar Shrinath Baliga, Ganesh Chandra Janetia, Shaival Kamalakasha Rao and Kiran Babu S. Evaluation of nitric oxide scavenging activity of certain spices in vitro :A preliminary study. Nahrung/Food. 2003:47(4);261-4.
  10. Jung-Yi Bor, Hui-Yin Chen, Gow-chin Yen. Evalution of antioxidant and Inhibitory effects on Nitric Oxide Production of Some Common Vegetables. J. Agric. Doof Chem. 54. 2006:1680-6.
  11. Park, YH. Effect of polyamine on modification of biommodics by aldehyde. PhD in Medicine Thesis. Seoul National University. 2000.
  12. 김숙희, 김화영. 老化. 서울.민음사. 1991:77-85, 94.
  13. 李獻平 外. 四大懷藥延綬衰老作用的硏究. 서울. 中西醫結合雜誌. 1991;11(8):486-7.
  14. Cutler, R. G. Antioxidant, aging and longevity. Free Radicals in Biology(ed. Pryor, W.). Acedimic Press. (6). 1984:371-424.
  15. 장석태. 피부과학. 서울. 여문각. 1994:23-5.
  16. Feher, J., Cosmos, G and Vereckei, A. The free radical theory of aging Free Radicals Reactions in Medicine. Springer-Verlag. Berlin. 1987:57-9.
  17. 리정복. 장수학. 과학백과사전출판사. 1987:11-99. 492-576.
  18. 김영곤, 김영균. 프리radical. 서울. 여문각. 1997:31-5, 98-101, 259-60, 278-86, 396-400, 425-6, 564-8.
  19. Blois MS. Antioxidant determination by the use of a stable free radical. Nature. 26. 1958:1198-120.
  20. Dimmeler S, Zeiher AM. Nitric oxide and apoptosis. Another paradigm for the double edged role of nitric oxide. Nitric oxide. 1997;1(41):275-81.
  21. Chung HT, Pae HO, Choi BM, Billiar T R, Kim YM. Nitric oxide as a bioregulator of apoptosis. Biochem biophys rescommum. 2001;282:1075-9.
  22. Monocada S, Higgs A. L-arginine nitric oxide pathway. NEng. J. Med. 1993;329:2002-12.
  23. Kim YM, Bombeck CA. Nitric oxide as a bifunctional regulator of apoptosis. Circ. res. 1999;1(4):253-6.
  24. Kim HY. Statistical study of acne vulgaris in korean adolescence. Kor. J. Dermatol. 1978;16:471-6.