DOI QR코드

DOI QR Code

Comparison of Chemical Composition and Radical Scavenging Activity of Pomegranate Extracts from Different Growing Areas

  • Lee, Hyun-Jung (Department of Food and Nutrition, Korea University) ;
  • Cho, Hye-Jin (Department of Food and Nutrition, Korea University) ;
  • Lee, Hyun-Sun (Institute of Health Science, Korea University) ;
  • Kwon, Ki-Han (Department of Complementary and Alternative Therapy, Gwangju University) ;
  • Hong, Yang-Hee (Department of Food and Nutrition, Donduk Women's University) ;
  • Kim, Seon-Hee (Department of Beauty and Coordiation, Suwon Science College) ;
  • Suh, Hyung-Joo (Department of Food and Nutrition, Korea University)
  • Published : 2009.09.30

Abstract

The pomegranate (Punica granatum L.) is a promising source of functional food, which contains several phytochemicals that perform important roles in reducing the risk of pathological diseases. Chemical compositions, such as the total sugar, uronic acid, polyphenols, and anthocyanin contents, and radical scavenging activity were determined and compared among PEs from six different cultivation areas. Total anthocyanin contents (TAC) and total polyphenol contents (TPC) from various growing areas were detected in the following order, respectively: Spain (19.08 ${\mu}g$/mL)> Turkey (12.91 ${\mu}g$/mL)> Iran-A (6.67 ${\mu}g$/mL)> Taiwan (4.77 ${\mu}g$/mL)> Uzbekistan (1.88 ${\mu}g$/mL)> Iran-B (0.76 ${\mu}g$/mL) and Turkey (639.52 ${\mu}g$/mL)> Uzbekistan (502.19 ${\mu}g$/mL)> Spain (306.40 ${\mu}g$/mL)> Iran-B (249.20 ${\mu}g$/mL)> Taiwan (162.78 ${\mu}g$/mL)> Iran-A (143.93 ${\mu}g$/mL). PEs from six different cultivation areas were determined to vary in ellagic acid content from 8.90 ${\mu}g$/mL to 332.52 ${\mu}g$/mL. The amounts of total sugars in PE from Iran-A evidenced relatively high total sugar contents, but low uronic acid levels (11.92 mg/mL). DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activities were as follows: Turkey> Uzbekistan $\gg$ Spain> Iran-B> Iran-A> Taiwan. ABTS [2,20-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)] radical scavenging activities were detected in the following order: Turkey$\geq$ Uzbekistan$\gg$ Spain> Iran-B> Iran-A> Taiwan. These results indicate that the PEs from Turkey with higher levels of TPC, TAC, ellagic acid, and higher radical scavenging activity may constitute a promising functional source for the prevention of several degenerative diseases.

Keywords

References

  1. Orak HH. 2009. Evaluation of antioxidant activity, colour and some nutritional characteristics of pomegranate (Punica granatum L.) juice and its sour concentrate processed by conventional evaporation. Int J Food Sci Nutr 60: 1-11 https://doi.org/10.1080/09637480701523306
  2. Lansky EP, Newman RA. 2007. Punica granatum (pomegranate) and its potential for prevention and treatment of inflammation and cancer. J Ethnopharmacol 109: 177-206 https://doi.org/10.1016/j.jep.2006.09.006
  3. Pendas J, Moreira T, Guerra O, Pena BR, Fernandez JA. 2001. Water relationships in Phyllantus orbicularis and Punica granatum antiviral extracts and their influence on stability after freezing and freeze-drying. Cryo Letters 22: 5-12
  4. Miguel G, Dandlen S, Antunes D, Neves A, Martins D. 2004. The effect of two methods of pomegranate (Punica granatum L.) juice extraction on quality during storage at $4^{\circ}C$. J Biomed Biotechnol 2004: 332-337 https://doi.org/10.1155/S1110724304403064
  5. Celik I, Temur A, Isik I. 2009. Hepatoprotective role and antioxidant capacity of pomegranate (Punica granatum) flowers infusion against trichloroacetic acid-exposed in rats. Food Chem Toxicol 47: 145-149 https://doi.org/10.1016/j.fct.2008.10.020
  6. Jurenka JS. 2008. Therapeutic applications of pomegranate (Punica granatum L.): a review. Altern Med Rev 13: 128-144
  7. Lako J, Trenerry VC, Wahlqvist M, Wattanapenpaiboon N, Sotheeswaran S, Premier R. 2007. Phytochemical flavonols, carotenoids and the antioxidant properties of a wide selection of Fijian fruit, vegetables and other readily available foods. Food Chem 101: 1727-1741 https://doi.org/10.1016/j.foodchem.2006.01.031
  8. Vinson JA, Dabbagh YA, Mamdouh MS, Jang J. 1995. Plant flavonoids, especially tea flavonols are powerful antioxidants using an in vitro oxidation model for heart disease. J Agric Food Chem 43: 2800-2802 https://doi.org/10.1021/jf00059a005
  9. Poyrazoglu E, Gökmen V, Artik N. 2002. Organic acids and phenolic compounds in pomegrantes (Punica granatum L.) grown in turkey. J Food Comp Anal 15: 567-575 https://doi.org/10.1016/S0889-1575(02)91071-9
  10. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. 1956. Colorimetric method for determination of sugars and related substances. Anal Chem 28: 350-356 https://doi.org/10.1021/ac60111a017
  11. Chen H, Zhang M, Xie B. 2004. Quantification of uronic acids in tea polysaccharide conjugates and their antioxidant properties. J Agric Food Chem 52: 3333-3336 https://doi.org/10.1021/jf0349679
  12. Quang DN, Hashimoto T, Nukada M, Yamamoto I, Tanaka M, Asakawa Y. 2003. Antioxidant activity of curtisians I-L from the inedible mushroom Paxillus curtisii. Planta Med 69: 1063-1066 https://doi.org/10.1055/s-2003-45159
  13. Wang LL, Xiong YL. 2005. Inhibition of lipid oxidation in cooked beef patties by hydrolyzed potato protein is related to its reducing and radical scavenging ability. J Agric Food Chem 53: 9186-9192 https://doi.org/10.1021/jf051213g
  14. Almajano MP, Carbo R, Delgado ME, Gordon MH. 2007. Effect of pH on the antimicrobial activity and oxidative stability of oil-in-water emulsions containing caffeic acid. J Food Sci 72: C258-263 https://doi.org/10.1111/j.1750-3841.2007.00387.x
  15. Gil MI, Tomas-Barberan FA, Hess-Pierce B, Holcroft DM, Kader AA. 2000. Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. J Agric Food Chem 48: 4581-4589 https://doi.org/10.1021/jf000404a
  16. Vardin H, Fenercioglu H. 2003. Study on the development of pomegranate juice processing technology: clarification of pomegranate juice. Nahrung 47: 300-303 https://doi.org/10.1002/food.200390070
  17. Lichtenthaler R, Marx F. 2005. Total oxidant scavenging capacities of common European fruit and vegetable juices. J Agric Food Chem 53: 103-110 https://doi.org/10.1021/jf0307550
  18. Zou JG, Wang ZR, Huang YZ, Cao KJ, Wu JM. 2003. Effect of red wine and wine polyphenol resveratrol on endothelial function in hypercholesterolemic rabbits. Int J Mol Med 11: 317-320
  19. Sestili P, Martinelli C, Ricci D, Fraternale D, Bucchini A, Giamperi L, Curcio R, Piccoli G, Stocchi V. 2007. Cytoprotective effect of preparations from various parts of Punica granatum L. fruits in oxidatively injured mammalian cells in comparison with their antioxidant capacity in cell free systems. Pharmacol Res 56: 18-26 https://doi.org/10.1016/j.phrs.2007.02.003
  20. Lansky EP. 2006. Beware of pomegranates bearing 40% ellagic acid. J Med Food 9: 119-122 https://doi.org/10.1089/jmf.2006.9.119
  21. Amakura Y, Okada M, Tsuji S, Tonogai Y. 2000. High-performance liquid chromatographic determination with photodiode array detection of ellagic acid in fresh and processed fruits. J Chromatogr A 896: 87-93 https://doi.org/10.1016/S0021-9673(00)00414-3
  22. Hilliam M. 1996. Functional foods: the western consumer viewpoint. Nutr Rev 54: S189-194 https://doi.org/10.1111/j.1753-4887.1996.tb03846.x
  23. Gao QP, Kiyohara H, Cyong JC, Yamada H. 1989. Chemical properties and anti-complementary activities of polysaccharide fractions from roots and leaves of Panax ginseng. Planta Med 55: 9-12 https://doi.org/10.1055/s-2006-961765
  24. Shin KS, Kiyohara H, Matsumoto T, Yamada H. 1997. Rhamnogalacturonan II from the leaves of Panax ginseng C.A. Meyer as a macrophage Fc receptor expressionenhancing polysaccharide. Carbohydr Res 300: 239-249 https://doi.org/10.1016/S0008-6215(97)00055-4
  25. Ozgen M, Durgac C, Serce S, Kaya C. 2008. Chemical and antioxidant properties of pomegranate cultivars grown in the Mediterranean region of Turkey. Food Chem 111: 703-706 https://doi.org/10.1016/j.foodchem.2008.04.043
  26. Moyer RA, Hummer KE, Finn CE, Frei B, Wrolstad RE. 2002. Anthocyanins, phenolics, and antioxidant capacity in diverse small fruits: vaccinium, rubus, and ribes. J Agric Food Chem 50: 519-525 https://doi.org/10.1021/jf011062r
  27. Velioglu YS, Mazza G, Gao L, Oomah BD. 1998. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J Agric Food Chem 46: 4113-4117 https://doi.org/10.1021/jf9801973
  28. Hertog MG, Sweetnam PM, Fehily AM, Elwood PC, Kromhout D. 1997. Antioxidant flavonols and ischemic heart disease in a Welsh population of men: the Caerphilly Study. Am J Clin Nutr 65: 1489-1494 https://doi.org/10.1093/ajcn/65.5.1489
  29. Ignarro LJ, Byrns RE, Sumi D, de Nigris F, Napoli C. 2006. Pomegranate juice protects nitric oxide against oxidative destruction and enhances the biological actions of nitric oxide. Nitric Oxide 15: 93-102 https://doi.org/10.1016/j.niox.2006.03.001
  30. Reddy MK, Gupta SK, Jacob MR, Khan SI, Ferreira D. 2007. Antioxidant, antimalarial and antimicrobial activities of tannin-rich fractions, ellagitannins and phenolic acids from Punica granatum L. Planta Med 73: 461-467 https://doi.org/10.1055/s-2007-967167
  31. Schubert SY, Lansky EP, Neeman I. 1999. Antioxidant and eicosanoid enzyme inhibition properties of pomegranate seed oil and fermented juice flavonoids. J Ethnopharmacol 66: 11-17 https://doi.org/10.1016/S0378-8741(98)00222-0
  32. Rasheed Z, Akhtar N, Anbazhagan AN, Ramamurthy S, Shukla M, Haqqi TM. 2009. Polyphenol-rich pomegranate fruit extract (POMx) suppresses PMACI-induced expression of pro-inflammatory cytokines by inhibiting the activation of MAP Kinases and NF-kappaB in human KU812 cells. J Inflamm (Lond) 6: 1-12 https://doi.org/10.1186/1476-9255-6-1

Cited by

  1. Fermenting Red Ginseng Enhances Its Safety and Efficacy as a Novel Skin Care Anti-Aging Ingredient:In Vitroand Animal Study vol.15, pp.11, 2012, https://doi.org/10.1089/jmf.2012.2187