Preventive Nutrition and Food Science

  • 제14권3호
  • /
  • Pages.260-264
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  • 2009
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  • 2287-1098(pISSN)
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  • 2287-8602(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
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Determination of Tocopherol Contents in Refined Edible Oils Using an HPLC Method

  • Hu, Jiang-Ning (Department of Food Science and Technology, Chungnam National University) ;
  • Zhu, Xue-Mei (Department of Food Science and Technology, Chungnam National University) ;
  • Adhikari, Prakash (Department of Food Science and Technology, Chungnam National University) ;
  • Li, Dan (Department of Food Science and Technology, Chungnam National University) ;
  • Kim, In-Hwan (Department of Food and Nutrition, Korea University) ;
  • Lee, Ki-Teak (Department of Food Science and Technology, Chungnam National University)
  • 발행 : 2009.09.30
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초록

A high-performance liquid chromatography method was applied to determine the contents of tocopherols in edible oils using a LiChrosorb DIOL HPLC column and hexane fortified with 0.1% acetic acid in an isocratic mode. The validation of the method included tests for linearity, sensitivity, accuracy, precision, and recovery. All calibration curves showed good linear regression ($r^2$>0.9995) within the tested ranges. The established method offered good precision and accuracy with overall intra-day and inter-day variations of 0.94$\sim$4.27 and 1.77$\sim$ 4.88%, respectively. The tocopherol recoveries ranged from 91.44$\sim$108.90%. Subsequently, the method was successfully applied to qualitatively and quantitatively determine the total contents of $\alpha$, $\gamma$, and $\delta$-tocopherols in 12 selected refined edible oils, showing a range of 0.92 to 188.71 mg/100 g.

키워드

참고문헌

  1. Isnardy B, Wagner KH, Elmadfa I. 2003. Effects of ${\alpha},\;{\gamma},\;and\;{\delta}$-tocopherols on the autoxidation of purified rapeseed oil triacylglycerols in a system containing low oxygen. J Agric Food Chem 51: 7775-7780 https://doi.org/10.1021/jf0348525
  2. Wagner KH, Elmadfa I. 2000. Effects of tocopherols and their mixtures on the oxidative stability of olive oil and linseed oil under heating. Eur J Lipid Sci Technol 102: 624-629 https://doi.org/10.1002/1438-9312(200010)102:10<624::AID-EJLT624>3.0.CO;2-I
  3. Kulas E, Ackman RG. 2001. Different tocopherols and the relationship between two methods for determination of primary oxidation products in fish oil. J Agric Food Chem 49: 1724-1729 https://doi.org/10.1021/jf0011541
  4. Jung MY, Min DB. 1990. Effects of ${\alpha},\;{\gamma},\;and\;{\delta}$ -tocopherols on oxidative stability of soybean oil. J Food Sci 55: 1464- 1465 https://doi.org/10.1111/j.1365-2621.1990.tb03960.x
  5. Satue MT, Huang SW, Frankel EN. 1995. Effect of natural antioxidants in virgin olive oil on oxidative stability of refined, bleached, and deodorized olive oil. J Am Oil Chem Soc 72: 1131-1137 https://doi.org/10.1007/BF02540978
  6. Kiokias S, Vazakas T, Oreopoulou V. 2008. In vitro activity of vitamins, flavonoids, and natural phenolic antioxidants against the oxidative deterioration of oil-based systems. Food Sci Nutr 48: 78-93 https://doi.org/10.1080/10408390601079975
  7. Nielsen NS, Pedersen A, Sandstrom B, Marckmann P, Hoy CE. 2002. Different effects of diets rich in olive oil, rapeseed oil and sunflower-seed oil on postprandial lipid and lipoprotein concentrations and on lipoprotein oxidation susceptibility. British J Nutr 87: 489-499 https://doi.org/10.1079/BJN2002567
  8. Sotiroudis TG, Kyrtopoulos SA, Xenakis A, Sotiroudis GT. 2003. Chemopreventive potential of minor components of olive oil against cancer. Italian J Food Sci 15: 169-185
  9. Leckbanda G, Frauena M, Friedt W. 2002. Rapeseed (Brassica napus) breeding for improved human nutrition. Food Res Intl 35: 273-278 https://doi.org/10.1016/S0963-9969(01)00196-X
  10. Reiter E, Jiang Q, Christen S. 2007. Anti-inflammatory properties of ${\alpha},\;and\;{\gamma}$ -tocopherol. Mol Aspects Med 28: 668-691 https://doi.org/10.1016/j.mam.2007.01.003
  11. Junker R, Kratz M, Neufeld M, Erren M, Nofer JR, Schulte H. 2001. Effects of diets containing olive oil, sunflower oil, or rapeseed oil on the hemostatic system. Thromb Haemost 5: 280-286
  12. Azadmard-Damirchi S, Dutta PC. 2008. Stability of minor lipid components with emphasis on phytosterols during chemical interesterification of a blend of refined olive oil and palm sterin. J Am Oil Chem Soc 85: 13-21 https://doi.org/10.1007/s11746-007-1170-1
  13. Tuberoso CIG, Kowalczyk A, Sarritzu E, Cabras P. 2007. Determination of antioxidant compounds and antioxidant activity in commercial oilseeds for food use. Food Chem 103: 1494-1501 https://doi.org/10.1016/j.foodchem.2006.08.014
  14. Gomez-Coronado DJM, Ibanez E, Ruperez FJ, Barbas C. 2004. Tocopherol measurement in edible products of vegetable origin. J Chrom A 1054: 227- 233 https://doi.org/10.1016/j.chroma.2004.08.072
  15. Manan F. 1994. Tocopherol contents of Pakistani seed oils studied by normal phase HPLC. J Islamic Acad Sci 7: 34-38
  16. Leenheer APD, Lambert WE, Ruyter MGMD. 1992. Modern chromatographic analysis of the vitamins. 3th ed. Marcel Deker, New York, USA. p 160
  17. Carlucci G, Mazzeo P, Governatore SD, Giacomo GD, Re GD. 2001. Liquid chromatographic method for the analysis of tocopherols in malt sprouts with supercritical fluid extraction. J Chrom A 935: 87-91 https://doi.org/10.1016/S0021-9673(01)01080-9
  18. Sanchez-Perez A, Delgado-Zamarreno MM, Bustanante- Rangel M, Hernandez-Mendez J. 2000. Automated analysis of vitamin E isomers in vegetable oils by continuous membrane extraction and liquid chromatography-electrochemical detection. J Chrom A 881: 229-241 https://doi.org/10.1016/S0021-9673(00)00314-9
  19. Gliszczynska-Swiglo A, Sikorska E. 2004. Simple reversed- phase liquid chromatography method for determination of tocopherols in edible plant oils. J Chrom A 1048: 195-198 https://doi.org/10.1016/j.chroma.2004.07.051
  20. Zhang FL, Wei YJ, Zhu J, Gong ZN. 2008. Simultaneous quantitation of three major triterpenoid glycosides in Centella asiatica extracts by high performance liquid chromatography with evaporative light scattering detection. Biomed Chromatogr 22: 119-124 https://doi.org/10.1002/bmc.901
  21. Sattler SE, Gilliland LU, Magallanes-Lundback M, Pollard M, DellaPenna D. 2004. Vitamin E is essential for seed longevity and for preventing lipid peroxidation during germination. Plant Cell 16: 1419-1432 https://doi.org/10.1105/tpc.021360
  22. Kaijeser A, Dutta P, Savage G. 2000. Oxidative stability and lipid composition of macadamia nuts grown in New Zealand. Food Chem 71: 67-70 https://doi.org/10.1016/S0308-8146(00)00132-1
  23. Cho EJ, Cho KH, Lee KT. 2005. Lipase-catalyzed synthesis of DHA-enriched structured lipid. Korean J Food Sci Technol 37: 709-712

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