Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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An Extrapolation Concentration Decision Effect Antihyperlipidemic of Aglycone Isoflavone from Biotransformation Soybean on the Fed High-Fat Diet Rats

생물전환법으로 만들어진 대두 비배당체 이소플라본을 섭취한 고지방 식이 쥐의 항고지혈 효과 및 결과에 의한 외삽적용 농도 결정

  • 임애경 ((재)대구테크노파크 바이오산업지원센터) ;
  • 정미정 (강원대학교 생명공학부) ;
  • 김동우 (내츄럴F&P) ;
  • 홍주헌 ((재)대구테크노파크 바이오산업지원센터) ;
  • 정희경 ((재)대구테크노파크 바이오산업지원센터) ;
  • 김길수 (경북대학교 수의과대학) ;
  • 김용해 (신화제약연구소(주)) ;
  • 김대익 ((재)대구테크노파크 바이오산업지원센터)
  • Published : 2009.09.30
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Abstract

An extrapolation of bioflavone through biotransformation used Phellinus banmill KCTC16882 applied to 6 different rats group were carried out. Male Sprague-Dawley rats were fed with bioflavone free (normal: NO) diet, high-fat diet (control: CO) and diets containing 0.35%, 0.7% and 3.0% isoflavone for 8 week. Hyperlipidemia was induced by adding lard 200 g and cholesterol 10 g to CO and 0.35, 0.7 and 3.0% bioflavone diet. Bioflavone diet arrested increases in body weight without affecting feed intake in the rats. BFP-3.0 treated group showed signigicant reduction in the serum level of TG, TC and LDL-cholesterol (p<0.05) compared to the CO, while HDL-cholesterol was increased (p<0.05). The atherogenic index, ALT and AST were decreased in the BFP treated groups (p<0.05). These results suggest that consumption of BFP may lead to extrapolation of an ameliortaion of metabolic syndromes as well as a reduction of cardiovascular disease and hyperlipidemia through increasing the of HDL-cholesterol, and decreasing the level of TG in serum.

상황버섯균주를 이용한 생물전환법으로 만들어진 바이오플라본의 항고지혈 활성에 따른 인체 외삽 적용을 위한 최소량을 살펴보고자 실험동물을 정상식이군(NO), 고지방 식이군(CO) 및 바이오플라본 식이첨가군(BFP-0.35, 0.7, 3.0)으로 나눈 후 8주간 사육하였다. 바이오플라본 첨가 식이는 실험동물에 식이섭취량을 감소시키고 체중의 증가를 억제 시켰으며, 장기 무게에 있어서도 간장과 복부지방에 있어서도 CO군에 비해 유의적으로 감소함을 확인할 수 있었다(p<0.05). 또한 총콜레스테롤, LDL-콜레스테롤, 중성지방 및 AI는 감소시키는데 반해 HDL-콜레스테롤은 증가하였다. 그리고 간 조직 손상정도를 나타내는 ALT 및 AST 효소에 있어서도 10% 이상의 효소감소 효과를 나타내었다. 이상과 같이 바이오플라본에는 고지혈로 야기되는 성인병 및 동맥경화지수를 감소시키는 생리활성 성분이 있는 것을 알 수 있으며 특히 모든 실험결과를 종합하였을 때 바이오플라본 0.35%, 0.7%, 3.0% 농도 중 3% 바이오플라본 첨가군에서 가장 큰 고지혈 감소 및 예방 효과를 나타내었다. 이로서 동물 실험 결과를 이용한 사람에게로의 외삽은 3% 농도로 제품을 개발하는 것이 효과적이라 사료된다. 또한 위의 결과를 바탕으로 바이오플라본이 항고지혈과 관련한 식품소재로서의 사용에 대한 가능성을 제시하는 바이다.

Keywords

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