Journal of Pharmacopuncture (대한약침학회지)

KOREAN PHARMACOPUNCTURE INSTITUTE (대한약침학회)
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Visualization of potential acupuncture points in rat and nude mouse and DiI tracing method

Trypan blue 도포를 사용한 누드 마우스와 흰쥐에서 경혈자리 찾기와 DiI 추적 법

  • Lee, Byung-Cheon (Biomedical Physics Laboratory for Korean Medicine, Department of Physics and Astronomy, Seoul National University) ;
  • Uhm, Ki-Hoon (Biomedical Physics Laboratory for Korean Medicine, Department of Physics and Astronomy, Seoul National University) ;
  • Bae, Kyoung-Hee (Biomedical Physics Laboratory for Korean Medicine, Department of Physics and Astronomy, Seoul National University) ;
  • Kang, Dae-In (Pharmacopuncture Med. Res. Center) ;
  • Soh, Kwang-Sup (Biomedical Physics Laboratory for Korean Medicine, Department of Physics and Astronomy, Seoul National University)
  • 이병천 (서울대학교 한의학 물리 연구실) ;
  • 엄기훈 (서울대학교 한의학 물리 연구실) ;
  • 배경희 (서울대학교 한의학 물리 연구실) ;
  • 강대인 ((주) 약침학회, 약침의학 연구소) ;
  • 소광섭 (서울대학교 한의학 물리 연구실)
  • Published : 2009.09.30
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Abstract

Objectives: To find the potential acupuncture points by using Trypan blue staining on the skin of rat and Nude mouse. Methods: 0.4% Trypan blue was applied to the skin of rat or Nude mouse previously treated by surfactant. Washing by warm saline was followed after enough application of trypan blue and surfactant. Frequency of Trypan blue application should be varied to the experimental animals' condition for visualizing significant spots. Results: Blue spots appeared roughly in symmetry along kidney meridian or stomach meridian. Several spots outside of kidney or stomach meridian were also observed; however, the detail stereoscopic images of those blue spots were slightly different according to the position blue-colored. DiI signals were visualized along blood vessel after DiI injection into the Trypan blue-visualized blue spots. Conclusion: Our method to visualize the potential acupuncture points as a blue spot on rat and Nude mouse skins may contribute to the next step for finding specific flowing channels among blue spots.

Keywords

References

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