Korean journal of food and cookery science (한국식품조리과학회지)

Korean Society of Food and Cookery Science (한국식품조리과학회)
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Antimutagenic and Cytotoxic Activity of Ethanol and Water Extracts from Rubus coreanum

복분자 딸기(Rubus coreanum) 에탄올 및 열수추출물의 항돌연변이 활성과 암세포 성장 억제 효과

  • Jeon, Yeon-Hee (Department of Food Science and Nutrition, Kyungpook National University) ;
  • Choi, Sang-Won (Department of Food Science and Nutrition, Catholic University of Daegu) ;
  • Kim, Mee-Ra (Department of Food Science and Nutrition, Catholic University of Daegu)
  • 전연희 (경북대학교 식품영양학과) ;
  • 최상원 (대구가톨릭대학교 식품영양학과) ;
  • 김미라 (경북대학교 식품영양학과.장수생활과학연구소)
  • Published : 2009.06.30
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Abstract

The antimutagenic and cytotoxic activities of ethanol and water extracts from Rubus coreanum were investigated in this study. Their antimutagenic activities were measured by the Ames test and their cytotoxic activities were evaluated by the growth inhibition of cancer cells via the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay and the sulforhodamine B (SRB) assay. In the results, the inhibition rates of the ethanol and water extracts toward mutagenicity induced by 4-NQO were 95.0% and 93.6% at 5 mg/plate, respectively, while their inhibition rates against mutagenicity induced by sodium azide were 27.2% and 40.8%, respectively. According to MTT assay, the cytotoxicity values of the ethanol extract against Hep3B and HeLa cells were 67.2% and 68.5%, respectively, and the values for the water extract were 65.8% and 66.4%, respectively. In the SRB assay, the ethanol and water extracts inhibited over 60% of cancer cell growth. In conclusion, both the ethanol and water extracts of Rubus coreanum offer potentially good antimutagenic and anticancer effects.

Keywords

References

  1. 배기환. 2000. 한국의 약용식물. 교학사. 서울. pp 127-129
  2. Ahn BY, Kim DG, Choi DS. 1999. Antimutagenic effect of Salvia Miltriorrhza Bunge. Kor J Appl Microbiol Biotechnol 27(3):197-202
  3. Branen AL. 1975. Toxicology and biochemistry of butylated hydroxy anisole and butylated hydroxy toluene. J Am Oil Chem Soc 52(2):59-63 https://doi.org/10.1007/BF02901825
  4. Carmichael J, DeGraff WG, Gazdar AF, Minna JD, Mitchell JB. 1987. Evaluation of a tetrazolium-based semiautomated colorimetric assay: Assessment of Chemosensitivity Testing. Cancer Res 47:936-942
  5. Cho EJ, Yang MO, Hwang CH, Kim WJ, Kim MJ, Lee MK. 2006. Quality characteristics of Sulfidduk added with Rubus coreanum Miquel during storage. J East Asian Soc Dietary Life 16(4):458-467
  6. Choi OK, Kim YS, Cho GS, Sung CK. 2002. Screening for antimicrobial activity from Korean Plants. Korean J Food & Nutr 15(4):300-306
  7. Doll R, Peto R. 1981. The causes of cancer: quantitative estimates of avoidable risks of cancer in the United States today. J Natl Cancer Inst 66(6):1191-1308
  8. Hwang Bo HS, Ham SS. 1999. Antimutagenic and cytotoxic effects of aster acaber root ethanol extract. Korean J Food Sci Technol 31(4):1065-1070
  9. Ito N, Fukushima S, Hasegawa A, Shibata M, Ogiso T. 1983. Carcinogenecity of butylated hydroxyanisole in F344 rats. J. Cancer Inst 70(22):343-347
  10. Iqbal S, Bhanger MI and Anwar F. 2005. Antioxidant properties and components of some commercially available varieties of rice bran in Pakistan. Food Chem. 93(2):265-272 https://doi.org/10.1016/j.foodchem.2004.09.024
  11. Kim DH, Park JH, Kim JH, Kim CH, You JH, Kwon MC, Lee HY. 2005. Enhancement of immune activities of Ephedrae herba and Rubi Fructus at low temperature extraction. Korean J Med Crop Sci 13(3):81-86
  12. Kim JH, Kim CH, Kim HS, Kwon MC, Song YK, Seong NS, Lee SE, Yi JS, Kwon OW, Lee HY. 2006. Effect of aqueous extracts from Rubus coreanus Miquel and Angelica gigas Nakai on Anti-tumor and Anti-stress activities in mice. Korean J Med Crop Sci 14(4):206-211
  13. Kim KH, Lee YA, Kim JS, Lee DI, Choi YW. 2000. Antioxidative activity of tannins from Rubus coreanum. Yakhak Hoeji 44(4):354-357
  14. Kim MJ, Kim JS, Kang WH, Jeong DM. 2002. Effect on antimutagenic and cancer cell growth inhibition of lxeris dentata nakai. Korean J Medicinal Crop Sci 10(2):139-143
  15. Kim MS, Pang GC, Lee MW. 1997. Flavonoids from the leaves of Rubus coreanum. Yakhak Hoeji 41(1):1-6
  16. Kim YH, Kang SS. 1993. Triterpenoids from Rubi Fructus (Bogbunja). Arch Pharn Res 16:109-113 https://doi.org/10.1007/BF03036856
  17. Kinae N, Masuda S. 2002. Studies on antimutagens (A review). Environ Mutagen Res 24(3): 129-144
  18. Lee KI, Park KY, Rhee SH. 1992. Antimutagenic effect of greenyellow vegetables toward aflatoxin B1 and 4-nitroquinoline-1-oxide. J Korean Soc Food Nutr 21(1):143-148
  19. Lee SE, Seong NS, Park CG, Seong JS. 2002. Screening for antioxidative activity of priental medicinal plant materials. Korean J Medicinal Crop Sci 10(3):171-176
  20. Lee MK, Lee HS, Choi GP, Oh DH, Kim JD, Yu CY, Lee HY. 2003. Screening of biological activities of the extracts from Rubus coreanus Miq. Korean J Medicinal Crop Sci 11(1):5-12
  21. Lee NJ, Lee KH, Lee DU, Park SS, Han YH, Ryu SY. 2001. Antimutagenic activity and cytotoxicity of the whole plant of Rumex acetosa. Korean J Pharmacogn 32(4):338-343
  22. Maron DM, Ames BN. 1983. Revised methods for the Salmonella mutagenicity test. Mut Res 113:173-215 https://doi.org/10.1016/0165-1161(83)90010-9
  23. Moon GS. 1991. Constituents and uses of medicinal herbs. Ilweolseogak. Seoul. pp. 310-311
  24. Nam SH, Jung JE, Kang MY. 1999. Screening of the mutagenicity and antimutagenicity of the hot water extracts from medicinal plants. J Korean Soc Agric Chem Biotechnol 42(4):344-350
  25. Nohmi T. 2002. Translesion DNA polymerases from microorganisms. Environ Mutagen Res 24(3):153-159
  26. Oh SW, Lee YC, Hong HD. 2002. Effects on the shelf-life Tofu with ethanol extracts of Rubus coreanus miquel, Therminalia chebula Retz and Rhus javanica. Korean J Food Sci Technol 34(4):746-749
  27. Park JG, Hyun JW, Lim KH and Shin JE. 1993. Antineoplastic effect of extracts from traditional medicinal plants. Korean J Pharmacogn. 24(3):223-230
  28. Park JH, Lee HS, Mun HC, Kim DH, Seong NS, Jung HG, Bang JK, Lee HY. 2004. Imptovement of anticancer activation of ultrasonificated extracts from acanthopanax senticosis Harms, Ephedra sinica Srapf, Rubus coreanus Miq. and Artemisia capillaris Thunb. Korean J Medicinal Crop Sci 12(4):273-278
  29. Park JH, Oh SM, Lim SS, Lee YS, Shin HK, Oh YS, Choe NH, Park JHY, Kim JK. 2006. Induction of heme oxygenase-1 mediates the anti-inflammatory effects of the ethanol extract of Rubus coreanus in murine macrophages. Biochemical and Biophysical Research Communications. 351(1):146-152 https://doi.org/10.1016/j.bbrc.2006.10.008
  30. Park YS. 2002. Antioxidative activities and contents of polyphenolic compound of medicinal herb extracts. J Fast Asian Soc Dietary Life 12(1):23-31
  31. Pauwels B, Pooter CMJ, Lambrechts HAJ, Lardon F. 2003. Comparision of the sulforhodamine B assay and the clonogenic assay for in vitro chemoradiation studies. Cancer Chemother Pharmacol 51:221-226
  32. Scudiero DA, Shoemaker RH, Paull KD, Monks A, Tierney S, Nofziger TH, Currens MJ. Seniff D, Boyd MR. 1998. Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines. Cancer Res 48:4827-4833
  33. Shin SM. 2008. Cytotoxic effects of korean black raspberry wine on various human cancer cells. Master thesis. Dogguk University. pp 2-31
  34. Song GS, Ahn BY, Lee KS, Maeng IK, Choi DS. 1997. Effect of hot water extracts from medical plants on the mutagenicity of indirect mutagens. Korean J Food Sci Technol 29(6):1288-1294