한국식품과학회지 (Korean Journal of Food Science and Technology)

한국식품과학회 (Korean Society of Food Science and Technology)
권호 표지

고초균에 의한 탈지대두 grits 발효물의 항혈전 및 콜레스테롤 저하 효과

Antithrombotic and Cholesterol Reduction Effects of Defatted Soybean Grits Fermented by Bacillus subtilis NUC1

  • 이성규 (계명대학교 식품가공학과) ;
  • 김현정 (계명대학교 전통 미생물자원 개발 및 산업화 연구센터) ;
  • 임남경 (계명대학교 식품가공학과) ;
  • 이은주 ((주)엔유씨전자 바이오 연구소) ;
  • 이삼빈 (계명대학교 식품가공학과) ;
  • 이인선 (계명대학교 식품가공학과)
  • Lee, Sung-Gyu (Department of Food and Technology, Keimyung University) ;
  • Kim, Hyun-Jeong (The Center for Traditional Microorganism Resources, Keimyung University) ;
  • Im, Nam-Kyung (Department of Food and Technology, Keimyung University) ;
  • Lee, Eun-Ju (Bio Research Institute, NUC Electronics Co. Ltd.) ;
  • Lee, Sam-Pin (Department of Food and Technology, Keimyung University) ;
  • Lee, In-Seon (Department of Food and Technology, Keimyung University)
  • 발행 : 2009.08.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구에서는 탈지대두 grits(DSG)와 B. subtilis NUC1균주로 발효시킨 탈지대두 발효물(FD)을 제조한 다음 열풍건조한 시료를 물 추출하여 이들 DSG 물추출물(DW)과 발효된 DSG 물추출물(FDW)의 항혈전 활성과 콜레스테롤 저하 효과를 알아보았다. 그 결과, FDW는 DW보다 항혈전 활성 및 혈소판의 응고 억제 효과가 더 우수하였으며, 유의적으로 높은 HMG-CoA reductase 저해활성을 가졌다. 그러나 DW와 FDW 처리군 둘다 HepG2 세포를 이용한 콜레스테롤 생합성 억제능을 보였고, 대조군에 비해 세포 내의 콜레스테롤 함량을 유의적으로 감소시켰다. 그리고 콜레스테롤 흡착능에서는 FDW만 약 90% 정도의 높은 흡착능을 보였고, DW는 콜레스테롤 흡착능을 보이지 않았다. 따라서 발효된 DSG는 원료인 DSG 보다 항혈전 및 콜레스테롤 개선 효과가 더 우수한 것으로 생각되었다.

This study investigated antithrombotic and hypocholesterolemic activities of defatted soybean grits (DSG) and fermented DSG (FD). The FD was prepared by the solid state fermentation using Bacillus subtilis NUC1 at $40^{\circ}C$ for 24h. The water extracts of fermented DSG (FDW) exhibited higher fibrinolytic activity and inhibition of platelet aggregation induced by ADP than water extracts of DSG (DW). However, the DW and FDW inhibited HMG-CoA reductase activity and significantly decreased the intracellular cholesterol contents in HepG2 cells. In addition, DW treatment did not show any cholesterol adsorption capacity, while FDW demonstrated the highest cholesterol adsorption by 90%. The results suggest that fermented DSG have significant antithrombotic and hypocholesterolemic effects in vitro and these activities were improved during fermentation by B. subtilis NUC1.

키워드

참고문헌

  1. NSOK. Annual Report on the Cause of Death Statistics. Natl. Stat. Office Korea, Seoul, Korea (2003)
  2. NSOK. The Expectancy of Future Population. Natl. Stat. Office Korea, Seoul, Korea (2002)
  3. Lim SS, Lee JH. Effect Atremisa Princeps var Orientals and Ccircium japonicum var ussuiense on serum lipid of hyperlipidemi crat. Korean J. Nutr. 30: 12-18 (1997)
  4. Mckenny JA. Lipid management: tools for getting to the goal. Am. J. Manag. Care 7: 299-306 (2001)
  5. Gang MS, Lim SS, Lee JH. A study on the chemical composition an hypocholesterilemic effect of nostoc commune. J. Korean Soc. Food Sci. Nutr. 26: 468-472 (1997)
  6. Choi HS, Ha JO, Choo MH, Na MS, Lee MY. Effect of bambusae caulis in liquamen on lipid metabolism in rats fed high fat diet. Korean J. Food Preserve 11: 373-382 (2004)
  7. Kang JA, Kang JS. Effect of garlic and onion on plasma an liver cholesterol and triglyceride and platelet aggregation in rate casal or cholesterol supplemented diets. Korean J. Nutr. 32: 132-138 (1997)
  8. Sitory CR. Pharmacology and mechanism of action of the new HMG-CoA reductase inhibitors. Pharm. Res. 22: 555-562 (1990) https://doi.org/10.1016/S1043-6618(05)80047-7
  9. Qureshi AA, Abuimeileh N, Din ZZ, Elson CE, Burger WC. Inhibition of cholesterol and fatty acid biosynthesis in liver enzymes and chicken hepatocytes by polar fractions of garlic. Lipids 18: 343-348 (1983) https://doi.org/10.1007/BF02537229
  10. Qureshi AA, Burger WC, Peterson DM, Elson C.E. Suppression of cholesterogenesis by plant constituents: review of wisconsin contributions to NC-167. Lipids 20: 817-824 (1985) https://doi.org/10.1007/BF02534407
  11. Stryer WH. Biochemistry, 4th ed. Freeman & Company, New York, USAL. pp. 685-712 (1995)
  12. Huff MW, Carrol KK. Effects of dietary proteins and amino acid mixtures on plasma cholesterol levels in rabbits. J. Nutr. 110: 1676-1685 (1980)
  13. Park MS, Liepa GU. Effects of dietary protein and amino acids on the metabolism of cholesterol-carring lipoproteins in rats. J. Nutr. 112: 1892-1898 (1982)
  14. Sautier C, Doucet C, Flament C, Lemonnier D. Effect of soy protein and saponins on serum, tissue and feces steroids in rats. Atherosclerosis 34: 233-241. (1979) https://doi.org/10.1016/S0021-9150(79)80004-0
  15. Sirtori CR, Agra E, Conti F, Gatti E, Mantero O, Tremili E, Sirtori M, Fraterrigo L, Tavazzi L, Kritxhevsky D. Clinical experience with the soybean protein diet in the treatment of hypercholesterolemia. Am. J. Clin. Nutr. 32: 1645-1658 (1979)
  16. Choi YB, Sohn HS. Isoflavone content in Korean fermented and unfermented soybean foods. Korean J. Food Sci. Technol. 30: 745-750 (1998)
  17. Hso S, Lee SK, Joo HK. Isolation and identification of fibrinolytic bacteria from Korean traditional chugkookjang. J. Korean Soc. Agric. Chem. Biotechnol. 41: 119-124 (1998)
  18. Kwon EY. Standardization of Chungkookjang preparation and its cancer preventive effect. MS thesis, Pusan National University, Pusan, Korea (2000)
  19. Yang JL. Antiatherogenic effect of chonkukjang. PhD thesis, Pusan National University, Pusan, Korea (2000)
  20. Kang MJ. Antibiabetic effect of soy pinito and Chongkukjang: Animal studies and clinical trials. PhD thesis, Inje University, Gimhae, Korea (2004)
  21. Shon MY, Seo KI, Park SK, Cho YS, Sung NJ. Some ciological activities and isoflavone content of Chungkugjang prepared with black beans and Bacillus strains. J. Korean Soc. Food Sci. Nutr. 30: 662-667 (2001)
  22. Byun MW, Son JH, Yook HS, Jo C, Kim DH. Effect of gamma irradiation on the physiological activity of Korean soybean fermented foods, Chungkookjang and Doenjang. Radiation Physics. and Chemistry 64: 245-248 (2002) https://doi.org/10.1016/S0969-806X(01)00492-3
  23. Astrup T, Mullertz S. The fibrin plate method for estimating fibrinolytic activity. Arch. Biochem. Biophys. 40: 346-351 (1952) https://doi.org/10.1016/0003-9861(52)90121-5
  24. Durand P, Bloy C, Peltier-Pujol F, Blache D. In-vitro and ex-vivo inhibition of blood platelet aggregation by naftazone. J. Pharm. Pharmacol. 48: 566-572 (1996) https://doi.org/10.1111/j.2042-7158.1996.tb05975.x
  25. Hulcher FH, Oleson WH. Simplified spectrophotometric assay for microsomal 3-hydroxy-3-methylglutaryl CoA reductase by measurement of coenzyme A. J. Lipid Res. 14: 625-631 (1973)
  26. Park SC, Noh YH, Koo J. Effects of ginseng components on content of cholesterol and activity of acyl CoA:cholesterol acyltransferase in HepG2 cells cultured in cholesterol rich medium. Korean J. Ginseng. Sci. 19: 212-218 (1995)
  27. Lowry OH, Rosebrough NH, Farr AL, Randall RJ. Protein measurement with folin phenol reagent. J. Biol. Chem. 193: 265-275 (1951)
  28. Soh HS, Kim CS, Lee SP. A new in vitro assay of cholesterol adsorption by food and microbial polysaccharides. J. Med. Food 6: 225-230 (2003) https://doi.org/10.1089/10966200360716643
  29. Miwa K, Kambara H, Kawai C. Effect of aspirin in large doses on attacks of variant angina. Am. Heart. J. 105: 351-355 (1983) https://doi.org/10.1016/0002-8703(83)90548-3
  30. Kim YT, Kim WK, Oh HI. Screening and identification of the fibrinolytic bacterial strain from Chungkook-jang. Korean J. Appl. Microbiol. Biotechnol. 23: 1-5 (1995)
  31. Fujita M, Nomura K, Hong K, Ito T, Asada A, Nishimura S. Purification and characterization of a strong fibrinolytic enzyme (nattokinase) in the vegetable cheese Natto, a popular soybean fermented food in Japan. Biochem. Biophys. Res. Commun. 197: 1340-1347 (1993) https://doi.org/10.1006/bbrc.1993.2624
  32. Oh HS, Park YH, Kim JH. Isoflavone contents, antioxidative and fibrinolytic activities of some commercial cooking-with-rice spyceans. Korean J. Food Sci. Technol. 34: 498-504 (2002)
  33. Kim WK, Choi KH, Kim YT, Park HH, Choi YJ, Lee YS, Oh HI, Kwon IB, Lee SY. Purification and characterization of a fibrinolytic enzyme produced from Bacillus sp. Strain CK 11-4 screened from Chungguk-jang. Applied and Environmental Microbiology 62: 2482-2488 (1996)
  34. Bainy EM, Tosh SM, Corredig M, Poysa V, Woodrow L. Varietal differences of carbohydrates in defatted soybean flour and soy protein isolate by-products. Carbohydrate Polymers 72: 664-672 (2008) https://doi.org/10.1016/j.carbpol.2007.10.008
  35. Armstrong RA. Platelet prostanoid receptors. Pharmacol. Ther. 72: 171-191 (1996) https://doi.org/10.1016/S0163-7258(96)00103-9
  36. Siess W. Molecular mechanism of platelet activation. Physiol. Rev. 69: 58-178 (1989)
  37. Endo A. The discovery and development of HMG-CoA reductase inhibitors. J. Lipid Res. 33: 1569-1582 (1992)
  38. Chen JR, Chiou SF, Sueteuna K, Yang HY, Yang SC. Lipid metabolism in hypocholesterolemic rats affected by feeding cholesterol-free diets containing different amounts of non dialyzed soybean protein fraction. Nutrition 19: 676-680 (2003) https://doi.org/10.1016/S0899-9007(03)00072-8
  39. Potter SM, Baum JA, Teng H, Stillman RJ, Shay NF, Erdman JW. Soy protein and isoflavones : their effects on blood lipids and bone density in postmenopausal women. Am. J. Clin. Nutr. 68: 1375-1379 (1998)
  40. Kim HJ, Lee SG, Ji YJ, Hwangbo MH, Lee EJ, Lee SP, Lee IS. Quality Characteristics of Defatted Soybean Grits Fermented by Bacillus Subtilis NUC1. J. Korean Soc. Food. Sci. Nutr. 37: 1479-1484 (2008) https://doi.org/10.3746/jkfn.2008.37.11.1479
  41. Kim YT, Kim WK, Oh HI. Screening and identification of the fibinolytic bacterial strain from Chongkukjang. Kor. J. Appl. Microbiol. Biotechnol. 23: 1-5 (1995)
  42. Kil JO, Kom GN, Park IS. Production and characterization of fibrinolytic enzyme: Optimal condition for production of the enzyme produced from Bacillus sp. KP-6408 isolated from Chongkukjang. J. Korean Soc. Food Sci. Nutr. 27: 51-56 (1998)