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옥수수 일차뿌리에서 TIBA가 brassinolide에 의해 유도된 굴중성 반응에 미치는 영향

Effect of TIBA on the Brassiolide-induced Gravitropic Response in the Primary Roots of Maize

  • Kang, Byung-Hee (Department of Biological Science, Andong National University) ;
  • Park, Jea-Hye (Department of Biological Science, Andong National University) ;
  • Kim, Jong-Sik (Department of Biological Science, Andong National University) ;
  • Jang, Soo-Chul (University College, Yonsei University) ;
  • Kim, Seung-Ki (Department of Life Science, Chung-Ang University) ;
  • Kim, Soon-Young (Department of Biological Science, Andong National University)
  • 발행 : 2009.08.30

초록

옥수수 뿌리에 BL을 처리하면 양성굴중성 반응이 촉진되고, ethylene 생성도 증가한다는 것이 알려져 있다. BL에 의해 유도된 굴중성 반응과 ethylene 생성과의 관계를 조사하였다. ethylene 생성 억제제인 $10^{-4}$ M AVG를 처리하면 ethylene 생성은 90% 이상 억제되었으나, 굴중성 반응은 13% 정도 억제되었다. AVG를 BL과 함께 처리한 뿌리는 ethylene 생성은 약 60% 억제되었으나 굴중성 반응은 대조구 보다 증가하였다. 다른 ethylene 생성 억제제인 cobalt ion을 처리하면 ethylene 생성은 약 10% 정도 억제되었으나 굴중성 반응은 억제되지 않았다. BL과 cobalt ion을 함께 처리한 뿌리는 ethylene 생성이 억제되었으나 굴중성 반응은 증가되었다. 이러한 BL의 효과가 auxin transport와 관계가 있는지 알아보기 위하여 auxin transport inhibitor인 TIBA를 처리하였다. $10^{-5}$ M TIBA와 BL과 TIBA를 함께 처리한 경우(BL+TIBA), ethylene 생성은 각각 96%, 132% 증가하였으나 굴중성 반응은 모두 일어나지 않았다. 또한, BL, TIBA 그리고 IAA를 함께 처리한 뿌리 (BL+TIBA+IAA)는 음성굴중성 반응을 나타냈으나 뿌리 생장은 오히려 증가시켰으며, 이는 수평으로 있는 뿌리에서 IAA가 아랫면으로 transport 되지 못하고 윗면에 축적된 것을 의미한다. 이러한 결과는 BL이 뿌리 내에 존재하는 IAA의 차등분포에 영향을 주어 양성굴중성 반응을 촉진할 가능성을 제시한다.

It has been known that brassiolide (BL) increased the positive gravitropic response and ethylene production in maize roots. This study examined the relationship between the BL-induced gravitropic response and ethylene Production. The ethylene production was inhibited to about 90% of the control by the treatment of $10^{-4}$ M aminoethoxyvinylglycine (AVG), the ethylene synthesis inhibitor. However, the gravitropic response did not show any significant changes compared to the control at $10^{-4}$ M AVG. In the case of treatment of AVG with BL, the ethylene production decreased to 60% of the control. However, the gravitropic response increased to the level which was induced by BL. Cobalt ions, another ethylene biosynthesis inhibitor, inhibited ethylene production, but not gravitropic response. When roots were treated with BL and cobalt ions, they showed the inhibition of ethylene production and promotion of gravitropic response. To elucidate the possibility that the effect of BL is related to auxin transport, roots were treated with TIBA (2,3,5-triiodobenzoic acid), an auxin transport inhibitor. Both treatment of TIBA alone and TIBA with BL stimulated ethylene production to about 96% and 132%, respectively. However, gravitropic response was completely inhibited in both treatments. Further, roots treated with BL in the presence of TIBA and IAA showed a negative gravitropic response, which means that IAA accumulates in the upper side of horizontal roots. Root elongation was also stimulated in this treatment. Taken together, these results suggest that BL might affect the differential distribution of internal IAA on roots, causing the regulation of positive gravitropic response.

키워드

참고문헌

  1. Abeles, F. B., P. W. Morgan, and M. E. Saltveit. 1992. Ethylene in Plant Biology. pp. 56-248, 2nd eds, Academic Press, San Diego, California
  2. Chang, S. C., Y. S. Kim, J. Y. Lee, P. B. Kaufman, A. Kirakosyan, H. S. Yun, T-W. Kim, S. Y. Kim, M. H. Cho, J. S. Lee, and S.-K. Kim. 2004. Brassinolide interacts with auxin and ethylene in the root gravitropic response of maize (Zea mays). Physiol. Plant. 121, 666-673 https://doi.org/10.1111/j.0031-9317.2004.00356.x
  3. Chen, R., E. Rosen, and P. H. Masson. 1999. Gravitropism in higher plants. Plant Physiol. 120, 343-350 https://doi.org/10.1104/pp.120.2.343
  4. Clouse, S. D. and J. M. Sasse. 1998. Brassinosteroid: essential regulators of plant growth and development. Ann. Rev. Plant Physiol. Plant Mol. Biol. 49, 427-451 https://doi.org/10.1146/annurev.arplant.49.1.427
  5. Fujioka, S. 1999. Natural occurrence of brassinosteroid on plant kingdom, pp. 21-45, In Sakorai, A., T. Yokota, and S. D. Clouse (eds.), Brassinosteroids, Steroidal Plant Hormones. Springer-Verlag, Tokyo
  6. Fujioka, S. and A. Sakurai. 1997. Brassinosteroids. Nat. Prod. Rep. 14, 1-10 https://doi.org/10.1039/np9971400001
  7. Grove, M. D., G. F. Spencer, W. K. Rohwedder, N. B. Mandava, J. F. Worley, J. D. Warthen Jr., S. L. Steffens, J. l. Flippen-Anderson, and J. C. Cook Jr. 1979. Brassinolide, a plant growth-promoting steroid isolated from Brassica napus pollen. Nature 281, 216-217 https://doi.org/10.1038/281216a0
  8. Kim, S. Y. and T. J. Mulkey. 1997a. Effect of ethylene antagonists on auxin-induced of intact primary root elongation in maize (Zea mays L.). J. Plant Biol. 40, 256-260 https://doi.org/10.1007/BF03030457
  9. Kim, S. Y. and T. J. Mulkey. 1997b. Effect of auxin and ethylene on elongation of intact primary roots of maize (Zea mays L.). J. Plant Biol. 40, 249-255 https://doi.org/10.1007/BF03030456
  10. Kim S-K., S. C. Chang, E. J. See, W. S. Chung, Y. S. Kim, S. B. Hwang, and J. S. Lee. 2000. Involvement of brassiosteorid in the gravitropic response of primary root of maize. Plant Physiol. 123, 997-1004 https://doi.org/10.1104/pp.123.3.997
  11. Kim, T.-W., S. M. Lee, S.-H. Joo, H. S. Yun, Y. Lee, P. B. Kaufman, A. Kirakosyan, S.-H. Kim, K. H. Nam, J. S. Lee, S. C. Chang, and S.-K. Kim. 2007. Elongation and gravitropic response of Arabidopsis roots are regulated by brassinolide and IAA. Plant Cell Envirn. 30, 679-689 https://doi.org/10.1111/j.1365-3040.2007.01659.x
  12. Lim, S. H., S. C. Chang, J. S. Lee, S-K. Kim, and S. Y. Kim. 2002. Brassinosteroids affects ethylene production in the primary roots of maize (ZeamaysL.). J. Plant Biol. 45, 148-153 https://doi.org/10.1007/BF03030307
  13. Lomax, T. L. 1997. Molecular genetic analysis of plant gravitropism. Gravitational Space Biol. 10, 75-82
  14. Meudt, W. J. 1987. Investigation on the mechanism of the brassinosteroid response. VI. Effect of brassinolide on gravitropism of bean hypocotyls. Plant Physiol. 83, 195-198 https://doi.org/10.1104/pp.83.1.195
  15. Park, W. J. 1998. Effect of epibrassinolide on hypocotyl growth of the tomato mutant diageotropica. Planta. 207, 120-124 https://doi.org/10.1007/s004250050463
  16. Perbal, G., D. DrissEcloe, M. Tewinkel, and D. Volkmann. 1997. Statocyte polarity and gravisensitivity in seedling roots grown in microgravity. Planta. 203, 57-62 https://doi.org/10.1007/PL00008115
  17. Sather, N. and T.-H. Iversen. 1991. Gravitropism and strach statolith in an Arabidopsis mutant. Planta. 184, 491-497 https://doi.org/10.1016/0273-1177(94)90381-6