Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Anxiolytic Effects of Woohwangcheongsimwon in Mice

  • Yoon, Byung-Hoon (Department of Life and Nanopharmaceutical Science, College of Pharmacy, Kyung Hee University) ;
  • Kim, Dong-Hyun (Department of Life and Nanopharmaceutical Science, College of Pharmacy, Kyung Hee University) ;
  • Lee, Seung-Joo (Department of Life and Nanopharmaceutical Science, College of Pharmacy, Kyung Hee University) ;
  • Shin, Bum-Young (Department of Pharmaceutical Science and Kyung Hee East-West Pharmaceutical Research Institute, College of Pharmacy, Kyung Hee University) ;
  • Lee, Yong-Hyuk (R & D Center, Kwang Dong Pharmaceutical Co., Ltd.) ;
  • Kim, Dong-Hee (R & D Center, Kwang Dong Pharmaceutical Co., Ltd.) ;
  • Park, Chan-Sung (R & D Center, Kwang Dong Pharmaceutical Co., Ltd.) ;
  • Lee, Yong-Wook (R & D Center, Kwang Dong Pharmaceutical Co., Ltd.) ;
  • Cho, Hi-Jae (R & D Center, Kwang Dong Pharmaceutical Co., Ltd.) ;
  • Yamamoto, Yutaka (Tochimoto Tenkaido Co., Ltd.) ;
  • Kang, Dong-Hyo (Tochimoto Tenkaido Co., Ltd.) ;
  • Ryu, Jong-Hoon (Department of Life and Nanopharmaceutical Science, College of Pharmacy, Kyung Hee University)
  • Published : 2009.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Woohwangcheongsimwon (WHCSW) is a traditional oriental medicinal fomula which has been clinically used for treating strokes, palpitation, loss of consciousness and anxiety. The purpose of this study was to characterize the putative anxiolytic properties of WHCSW using an elevated plus-maze (EPM) and hole-board test. Control mice were orally treated with an equal volume of vehicle (10% Tween 80 solution), and positive control mice were treated with diazepam (1 mg/kg, i.p.). In the EPM test, WHCSW significantly increased the percentage of time-spent in the open arms (200 mg/kg, P < 0.05) and the percentage of open arm entries (200 and 400 mg/kg, P < 0.05). WHCSW also significantly increased the number of head-dips in the hole-board test (200 mg/kg, P < 0.05). In addition, the anxiolytic properties of WHCSW examined in the EPM test were inhibited by flumazenil (10 mg/kg, i.p.), a GABA$_A$ antagonist. However, no changes in spontaneous locomotor activity or myorelaxant effects were observed versus 10% Tween 80 controls. These results suggested that WHCSW is an effective anxiolytic agent, and that its anxiolytic effects are mediated via GABA$_A$ receptors.

Keywords

References

  1. Ambawade, S.D., Kasture, V.S., and Kasture, S.B., Anxiolytic activity of Glycyrrhiza glabra Linn. J. Nat. Rem. 2, 130-134 (2001) https://doi.org/10.1038/35052563
  2. Bhattacharya, S.K. and Mitra, S.K., Anxiolytic activity of Panax ginseng roots: an experimental study. J. Ethnopharmacol. 34, 87-92 (1991) https://doi.org/10.1016/0378-8741(91)90193-H
  3. Bonnetti, E.P., Pieri, L., Cumin, R., Schaffner, R., Pieri, M., Gamzu, E.R., Muller, R.K.M., and Haefely, W.. Benzodiazepine antagonist Ro 15- 1788: neurological and behavioral effects. J. Psychopharmacol. 78, 8- 18 (1982) https://doi.org/10.1007/BF00470579
  4. Cabib, S., Algeri, S., Perego, C., and Puglisi-Allegra, S., Behavioral and biochemical changes monitored in two inbred strains of mice during exploration of an unfamiliar environment. Physiol. Behav. 47, 749-753 (1990) https://doi.org/10.1016/0031-9384(90)90089-M
  5. Carlini, E.A., Plants and the central nervous system. Pharmacol. Biochem. Behav. 75, 501-512 (2003) https://doi.org/10.1016/S0091-3057(03)00112-6
  6. Chen, S.W., Min, L., Li, W.J., Kong, W.X., Li, J.F., and Zhang, Y.J., The effects of angelica essential oil in three murine tests of anxiety. Pharmacol. Biochem. Behav. 79, 377-382 (2004) https://doi.org/10.1016/j.pbb.2004.08.017
  7. Dawson, G.R. and Tricklebank, M.D., Use of elevated plus maze in the search for novel anxiolytic agents. Trends Pharmacol. Sci. 16, 33-36 (1995) https://doi.org/10.1016/S0165-6147(00)88973-7
  8. Dunn, R.W., Corbett, R., and Fielding, S., Effects of 5-HT1A receptor agonists and NMDA receptor antagonists in the social interaction test and elevated plus maze. Eur. J. Pharmacol. 169, 1-10 (1989) https://doi.org/10.1016/0014-2999(89)90811-X
  9. Handley, S.R. and Tricklebank, M.D., Effects of alpha-adrenoceptor agonists and antagonists in a maze-exploration model of 'fear'- motivated behaviour. Naunyn Schmiedebergs Arch. of Pharmacol. 327, 1-5 (1995) https://doi.org/10.1007/BF00504983
  10. Hui, K.M., Huen, M.S., Wang, H.Y., Zheng, H., Sigel, E., Baur, R., Ren, H., Li, Z.W., Wong, J.T., and Xue, H., Anxiolytic effect of wogonin, a benzodiazepine receptor ligand isolated from Scutellaria baicalensis Georgi. Biochem. Pharmacol. 64, 1415-1424 (2002) https://doi.org/10.1016/S0006-2952(02)01347-3
  11. Jung, J.W., Ahn, N.Y., Park, S.H., Oh, J.K., Oh, H.R., Lee, B.K., Om, A.S., Kim, B.S., Kim, D.H., and Ryu, J.H., The anxiolytic-like effect of Scutellaria Baicalensis using elevated plus-maze in rat. Kor. J. Pharmacogn. 35, 22-27 (2004).
  12. Jung, J.W., Yoon, B.H., Oh, H.R., Ahn, J.H., Kim, S.Y., Park, S.Y., and Ryu, J.H., Anxiolytic-like effects of Gastrodia elata and its phenolic constituents in mice. Biol. Pharm. Bull. 29, 261-265 (2006a) https://doi.org/10.1248/bpb.29.261
  13. Jung, J.W., Ahn, N.Y., Oh, H.R., Lee, B.K., Lee, K.J., Kim, S.Y., Cheong, J.H., and Ryu, J.H., Anxiolytic effects of the aqueous extract of Uncaria rhynchophylla. J. Ethnopharmacol. 108, 193-197 (2006b) https://doi.org/10.1016/j.jep.2006.05.019
  14. Klint, T., Effect of 8-OH DPAT and buspirone in a passive avoidance test and in the elevated plus maze test in rats. Behav. Pharmacol. 2, 481-489 (1991)
  15. Lader, M. and Morton, S., Benzodiazepine problems. Br. J. Addict. 86, 823-828 (1991) https://doi.org/10.1111/j.1360-0443.1991.tb01831.x
  16. Lepine, J.P., The epidemiology of anxiety disorders: prevalence and societal costs. J. Clin. Psychiatry 63, Suppl 14, 4-8 (2002) https://doi.org/10.4088/JCP.v63n0101
  17. Lister, R.G., The use of a plus-maze to measure anxiety in the mouse. Psychopharmacology (Berl). 92, 180-185 (1987)
  18. Min, L., Chen, S.W., Li, W.J., Wang, R., Li, Y.L., Wang, W.J., and Mi, X.J., The effects of angelica essential oil in social interaction and holeboard tests. Pharmacol. Biochem. Behav. 81, 838-842 (2005) https://doi.org/10.1016/j.pbb.2005.05.015
  19. Moser, PC., An evaluation of the elevated plus-maze test using the novel anxiolytic buspirone. Psychopharmacology (Berl). 99, 48-53 (1989) https://doi.org/10.1007/BF00634451
  20. Newton, R.E., Marunycz, J.D., Alderdice, M.T., and Napoliello, M.J., Review of the side-effect profile of buspirone. Am. J. Med. 80, 17-21 (1986) https://doi.org/10.1016/0002-9343(86)90327-X
  21. O'brien, CP., Benzodiazepine use, abuse, and dependence. J. Clin. Psychiatry 66, Suppl 2, 28-33 (2005)
  22. Pellow, S., Chopin, P., File, S.E., and Briley, M., Validation of open: closed arm entries in an elevated plus-maze as a measure of anxiety in the rat. J. Neurosci. Methods 14, 149-167 (1985) https://doi.org/10.1016/0165-0270(85)90031-7
  23. Peng, W.H., Hsieh, M.T., Lee, Y.S., Lin, Y.C., and Liao, J., Anxiolytic effect of seed of Ziziphus jujuba in mouse models of anxiety. J. Ethnopharmacol. 72, 435-441 (2000) https://doi.org/10.1016/S0378-8741(00)00255-5
  24. Rodriguez Echandia, E.L., Broitman, S.T., and Foscolo, M.R., Effect of the chronic ingestion of chlorimipramine and desipramine on the hole board response to acute stresses in male rats. Pharmacol. Biochem. Behav. 26, 207-210 (1987) https://doi.org/10.1016/0091-3057(87)90106-7
  25. Scholey, A., Kennedy, D., and Wesnes, K., The psychopharmacology of herbal extracts: issues and challenges. Psychopharmacology (Berl) 179, 705-707; author reply 708-711 (2005) https://doi.org/10.1007/s00213-004-2062-9
  26. Takeda, H., Tsuji, M., and Matsumiya, T., Changes in head-dipping behavior in the hole-board test reflect the anxiogenic and/or anxiolytic state in mice. Eur. J. Pharmacol. 350, 21-29 (1998) https://doi.org/10.1016/S0014-2999(98)00223-4
  27. Trent, D.L. and Edwin, D.L., Dietary soy phytoestrogens produce anxiolytic effects in the elevated plus-maze. Brain Res. 913, 180-184 (2001) https://doi.org/10.1016/S0006-8993(01)02793-7
  28. Vishwakarma, S.L., Pal, S.C., Kasture, V.S., and Kasture, S.B., Anxiolytic and antiemetic activity of Zingiber officinale. Phytother. Res. 16, 621-626 (2002) https://doi.org/10.1002/ptr.948
  29. Wada, T. and Fukuda, N., Effects of DN-2337, a new anxiolytic, diazepam and buspirone on exploratory activity of the rat in an elevated plus maze. Psychopharmacology (Berl) 104, 444-450 (1991) https://doi.org/10.1007/BF02245647
  30. Wang, H., Hui, K.M., Xu, S., Chen, Y., Wong, J.T., and Xue, H., Two flavones from Scutellaria baicalensis Georgi and their binding affinities to the benzodiazepine site of the GABAA receptor complex. Pharmazie 57, 857-858 (2002)
  31. Whiting, P.J., GABA-A receptors: a viable target for novel anxiolytics? Curr, Opin. Pharmacol. 6, 24-29 (2006) https://doi.org/10.1016/j.coph.2005.08.005