Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Genetic Polymorphism of Microsatellite Markers in Panax ginseng C.A. Meyer

인삼 (Panax ginseng C.A. Meyer)의 Microsatellite 마커에 대한 유전적 다형성과 특성 규명

  • Park, Sun-Wha (Department of Biological Science, Kongju National University) ;
  • Hyun, Young-Se (Department of Biological Science, Kongju National University) ;
  • Chung, Ki-Wha (Department of Biological Science, Kongju National University)
  • Published : 2009.09.30
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Abstract

Ginseng (Panax ginseng C.A. Meyer) is one of the most important medicinal plants in East Asia. Microsatellite or simple sequence repeat (SSR) markers are used in obtaining genetic analysis and authentication in many plants. The present study examined five microsatellites in conjunction with P. ginseng in Korea. The total observed allelic number was 17 (mean = 3.4), and gene diversities varied from 0.078 to 0.543 with an average of 0.314. Through a combined analysis of five loci in 100 ginseng samples, 44 different combined genotypes were observed. Expected and observed heterozygosites ranged from 0.077 to 0.541 (mean = 0.313) and 0.040 to 0.130 (0.083), respectively. All examined loci exhibited deficiency of heterozygosity and deviation from the Hardy-Weinberg equilibrium. Such results may be explained by the non-random mating and inbreeding that has occurred for several hundred years. These microsatellite markers could be used for the study of molecular genetics and the establishment of DNA marker database, as well as authentication of ginseng species and chromosomal mapping of QTL loci in P. ginseng.

인삼에 대한 microsatellite 개발은 다른 분자적 마커들에 비해 늦게 이루어져, 최근에 와서야 인삼의 microsatellite 들이 보고되고 있는 실정이다. 본 연구에서는, 분리된 microsatellite들 중에서 5 개의 다형성 마커를 선별하여 국내 경작지나 시장에서 유통되는 인삼을 대상으로 유전적 다형성을 조사하고, 각 마커의 특성을 규명하였다. 유전자형 분석은 변성 PAGE와 silver staining법으로 하거나 형광표지 primer로 표지한 PCR 산물을 자동 염기서열 분석기로 분석하였다. 본 연구에서 개발한 5개의 microsatellite 마커들의 평균 대립유전자 수는 3.2 개였으며, 평균 GD는 0.367 였다. 전체적으로 볼 때, PG1419가 가장 높은 다형성을 보였으며 (PIC: 0.460, GD: 0.543), PG770은 가장 낮은 다형성을 나타내었다 (PIC: 0.070, GD: 0.078). 각 좌위들의 예상 이형접합도 (H$_{exp}$)는 0.077에서 0.541 (mean = 0.313)로 계산되었으나, 관측 이형접합도 (H$_{obs}$)는 0.040에서 0.130 (mean = 0.083)으로 훨씬 낮게 관찰되었으며, 유전자형의 분포는 Hardy-Weinberg 평형상태에서 벗어남을 보였다. 본 연구에서 개발한 인삼의 microsatellite 마커들은 인삼의 분자적 마커의 데이터베이스 확립의 기초 자료로 활용될 뿐 아니라, 인삼의 분자적 구별법 및 QTL 좌위의 염색체지도 작성에 유용하게 활용될 것이다.

Keywords

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