Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
권호 표지

Immunomodulatory activity of Scutellaria baicalensis Georgi Pharmacopuncture on Acute Inflammatory Rat Model

황금(黃芩) 약침(藥鍼)이 급성염증 백서의 면역조절능에 미치는 영향

  • Yang, Seung-Hee (Dept. of Meridian & Acupoint, College of Korean Medicine, Sangji University) ;
  • Lee, Hyang-Sook (Dept. of Meridian & Acupoint, College of Korean Medicine, Sangji University) ;
  • Lee, Eun (Dept. of Pharmaceutical Engineering, College of health Science, Sangji University) ;
  • Lee, Joon-Moo (Dept. of Meridian & Acupoint, College of Korean Medicine, Sangji University)
  • 양승희 (상지대학교 한의과대학 경락경혈학교실) ;
  • 이향숙 (상지대학교 한의과대학 경락경혈학교실) ;
  • 이은 (상지대학교 보건과학대학 제약공학과) ;
  • 이준무 (상지대학교 한의과대학 경락경혈학교실)
  • Published : 2009.09.27
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : To investigate the anti-inflammatory effects of Scutellaria baicalensis Georgi pharmacopuncture in lipopolysaccharide (LPS)-induced inflammatory rat model. Methods : Sprague-Dawley rats were divided into 4 groups; LPS control (n=6), LPS+Scutellaria baicalensis Georgi pharmacopuncture at BL23 (n=6, BL23), LPS+Scutellaria baicalensis Georgi pharmacopuncture at CV12 (n=6, CV12), and LPS+Scutellaria baicalensis Georgi pharmacopuncture at GV4 (n=6, GV4). Pharmacopuncture was given every two days for 4 weeks followed by inflammation induction by intraperitoneal LPS injection (5mg/kg). Blood, liver tissue, and peritoneal lavage fluid were taken and proinflammatory cytokines and other related factors were analysed. Results : For proinflammatory cytokines, CV12 pharmacopuncture group was significantly different compared with the control group in plasma IL-$1{\beta}$, IL-6, TNF-$\alpha$, and IL-10 5 h after LPS injection (P<0.05). For plasma IL-$1{\beta}$ and IL-6, CV12 pharmacopuncture group also showed significant difference at 2 h compared with the control (P<0.05). GV4 pharmacopuncture group was significantly different compared with the control at 5 h in plasma IL-$1{\beta}$, IL-6, and TNF-$\alpha$ and at 2 h in IL-10 (P<0.05). Liver cytokines were analyzed at 5 h after LPS injection; only CV12 pharmacopuncture group showed significant difference in IL-$1{\beta}$ (P<0.05) and others including IL-6, TNF-$\alpha$, and IL-10 had no difference compared with the control group. CD4/CD8 ratio and the phagocytic activities of polymorphonuclear neutrophils were not different from those of control group in all pharmacopuncture groups (P>0.05). Plasma NO3-/NO2- and intracellular adhesion molecule-1 of CV12 pharmacopuncture group were significantly lower than that of the control group (P<0.05). In the plasma concentration of prostaglandin E2, all 3 pharmacopuncture groups had significantly lower values than that of the control group (P<0.05), but there was no difference among pharmacopucnture groups. Monocyte chemoattractant protein-1 and cytokine-induced neutorphil chemoattractant-1 in peritoneal lavage fluid was significantly decreased in CV12 pharmacopuncture group compared with the control group (P<0.05). Conclusions : These results indicate that Scutellaria baicalensis Georgi pharmacopuncture at CV12 may have a potent anti-inflammatory effect in an LPS-induced inflammatory rat model.

Keywords

References

  1. Rabson A, Roitt IM, Delves PJ. Really Essential Medical Immunology. Oxford : Blackwell Publishing Ltd. 2005 : 1-14.
  2. 김현제 외 편역. 한의학사전. 서울 : 성보사. 1988 : 194.
  3. 문류모. Stress에 관한 문헌적 고찰 - 현대의학을 중심으로. 동의신경정신과학회지. 1991 ; 2(1) : 38-50.
  4. 채병윤. 한방외과. 서울 : 고문사. 1978 : 53.
  5. 김호철. 한약약리학. 서울 : 집문당. 2001 : 129-33.
  6. Middleton E Jr, Drzewiecki G. Naturally occurring flavonoids and human basophil histamine release. Int Arch Allergy Appl Immun. 1985 ; 77(1-2) : 155-7. https://doi.org/10.1159/000233771
  7. Middleton E Jr, Harborne JB, Beretz A. Plant Flavonoids in Biology and Medicine II. Biochemical, Cellular and Medicinal Properties. New York : Alan R. Liss. 1988 : 61-5.
  8. 최영광, 박성호, 서일복, 김호현, 김정선, 김이화. 黃芩藥鍼液이 LPS로 유발된 RAW 264.7 대식세포에서의 항염증효과. 대한약침학회지. 2003 ; 6(3) : 5-14.
  9. WHO standard acupuncture point locations in the western pacific region. WHO Library Cataloguing in Publication Data. 2008 : 111, 205, 225.
  10. Bohmer RH, Trinkle LS, Staneck JL. Dose effects of LPS on neutrophils in a whole blood flow cytometric assay of phagocytosis and oxidative burst. Cytometry 1992 ; 13(5) : 525-31. https://doi.org/10.1002/cyto.990130512
  11. Hong T, Jin GB, Cho S, Cyong JC. Evaluation of the anti-inflammatory effect of baicalein on dextran sulfate sodium-induced colitis in mice. Planta Med. 2002 ; 68(3) : 268-71. https://doi.org/10.1055/s-2002-23143
  12. 이순희, 임병우, 조여원. DSS로 유도된 염증성 장 질환 동물 모델에서 황금 열수 추출물이 면역 조절 기능에 미치는 영향. 한국영양학회지. 2004 ; 37(6) : 431-9.
  13. 김유승, 송춘호. 黃芩藥鍼이 type 1 hypersensitivity에 미치는 영향. 경락경혈학회지. 2006 ; 23(3) : 111-22.
  14. 전국한의과대학, 한의학전문대학원 경락경혈학 교재편찬위원회. 대학경락경혈학각론(상, 하). 의방서원. 2009 : 591, 1172, 1234.
  15. 唐照亮. 灸疗抗炎免疫作用的实验研究. 中國鍼灸. 1997 ; 17(4) : 233.
  16. 김동윤, 김경호, 김갑성, 송춘호, 안창범. 胃兪穴과 中脘穴의 蒼朮水鍼이 胃炎回復 效果에 미치는 影響. 대한침구학회지. 1993 ; 10(1) : 289-96.
  17. Marriott JB, Westby M, Cookson S, Guckian M, Goodbourn S, Muller G et al. CC-3052: A water-soluble analog of thalidomide and potent inhibitor of activation-induced TNF-$\alpha$ production. J Immunol. 1998 ; 161(8) : 4236-43.
  18. Alba-Loureiro TC, Martins EF, Landraf RG, Jancar S, Curi R, Sannomiya P. Role of insulin on PGE2 generation during LPS-induced lung inflammation in rats. Life Sci. 2006 ; 78(6) : 578-85. https://doi.org/10.1016/j.lfs.2005.05.057
  19. Simpson KJ, Lukacs NW, Colletti L, Strieter RM, Kunkel SL. Cytokines and the liver. J Hepatol. 1997 ; 27(6) : 1120-32. https://doi.org/10.1016/S0168-8278(97)80160-2
  20. Mathiak G, Grass G, Herzmann T, Luebke T, Zetina CC, Boehm SA et al. Capase-1-inhibitor ac-YVAD-cmk reduces LPS-lethality in rats without affecting haematology or cytokine responses. Br J Pharmacol. 2000 ; 131(3) : 383-6. https://doi.org/10.1038/sj.bjp.0703629
  21. Chamulitrat W, Blazka ME, Jordan SJ, Luster MI, Mason RP. Tumor necrosis factor-$\alpha$ and nitric oxide production in endotoxin-primed rats administered carbon tetrachloride. Life Sci. 1995 ; 57(24) : 2273-80. https://doi.org/10.1016/0024-3205(95)02220-D
  22. Harbrecht BG, Di Silvio M, Demetris AJ, Simmons RL, Billiar TR. Tumor necrosis factor-$\alpha$ regulates in vivo nitric oxide synthesis and induces liver injury during endotoxemia. Hepatology. 1994 ; 20(4 Pt 1) : 1055-60. https://doi.org/10.1002/hep.1840200439
  23. Thompson KC, Trowern A, Fowell A, Marathe M, Haycock C, Arthur MJ et al. Primary rat and mouse hepatic stellate cells express the macrophage inhibitor cytokine interleukin-10 during the course of activation in vitro. Hepatology. 1998 ; 28(6) : 1518-24. https://doi.org/10.1002/hep.510280611
  24. Tsou SS, Chiu WC, Yeh CL, Hou YC, Yeh SL. Effects of omega-3 fatty acids on inflammatory mediators and splenocyte cytokine mRNA expressions in rats with polymicrobial sepsis. Nutrition. 2008 ; 24(5) : 484-91. https://doi.org/10.1016/j.nut.2008.01.012
  25. Harris SG, Padilla J, Koumas L, Ray D, Phipps RP. Prostaglandins as modulators of immunity. Trends Immunol. 2002 ; 23(3) : 144-50. https://doi.org/10.1016/S1471-4906(01)02154-8
  26. Hogg N. Free radicals in disease. Semin Reprod Endocrinol. 1998 ; 16(4) : 241-8. https://doi.org/10.1055/s-2007-1016284
  27. Bressan E, Cunha F de Q, Tonussi CR. Contribution of TNF-alpha, IL-1 beta and CINC-1 for articular incapacitation, edema and cell migration in a model of LPS-induced reactive arthritis. Cytokine. 2006 ; 36(1-2) : 83-9. https://doi.org/10.1016/j.cyto.2006.11.007
  28. 김성일, 도원석, 김갑성. 黃芩藥鍼液의 흰쥐 肝細胞內의 抗酸化 效能에 관한 硏究. 대한침구학회지. 1993 ; 16(1) : 497-509.