Reproducibility Between two physicians of fMRI study on the Brain Activity Induced by Acupuncture (at BL62)

  • Yeo, S. (Meridian and Acupoint, Grad. Sch. of Basic Eastern Med. Science, Kyung Hee Univ.) ;
  • Kim, Y. (Meridian and Acupoint, Grad. Sch. of Basic Eastern Med. Science, Kyung Hee Univ.) ;
  • Choe, I.H. (Applied Meridian Med., Grad. Sch. of Applied Eastern Medicine, Kyung Hee Univ.) ;
  • Rheu, K.H. (Applied Meridian Med., Grad. Sch. of Applied Eastern Medicine, Kyung Hee Univ.) ;
  • Choi, Y.G. (Acupuncture and Meridian, WHO Collaborating Ctr. for Traditional Medicine, East-West Med. Res. Institute, Kyung Hee Univ.) ;
  • Hong, Y.M. (Meridian and Acupoint, Grad. Sch. of Basic Eastern Med. Science, Kyung Hee Univ.) ;
  • Lim, S. (Meridian and Acupoint, Grad. Sch. of Basic Eastern Med. Science, Kyung Hee Univ.)
  • 발행 : 2009.06.27

초록

목적 : 신맥 혈위 자극이 뇌활성화 변화에 미치는 영향에 있어 자침 시술자간의 재현성을 fMRI 영상을 통해 평가하고자 하였다. 방법 : 본 연구는 건강한 성인 남자 15명을 대상으로 실시하였으며, 15명의 피험자 중 7명은 각각 2회 방문하였으며, 두 시술자에 의해 침을 맞고 총 4개의 데이터를 얻었다. 나머지 8명은 1회 방문하여 두 시술자에게 침을 맞아 2개씩의 데이터를 얻어 총 44개의 fMRI 데이터를 얻었다. 실험자간의 차이를 줄이기 위해 자침의 깊이와 회전, 강도 등을 동일하게 하였으며, 우측 신맥혈에 자침하였다. 침에 의해 활성화되는 영역을 확인하기 위해 블록디자인을 사용하여 fMRI를 촬영하였다. 결과 : 다른 날에 실시한 같은 시술자내의 재현성은 24 %, 같은 날 실시한 다른 시술자간의 재현성은 64 % 로, 다른 시술자간의 재현성이 서로 다른 날 실시한 같은 시술자내의 재현성보다 높게 나타났다. 결론 : 침을 이용한 fMRI의 실험에서 시술자에 의한 차이 외에도 실험하는 날짜의 차이, 피험자 인체의 생리적인 변화 등에 의한 차이가 크다는 것을 본 실험을 통하여 확인하였다. 그리고 자침의 깊이와 회전, 자극의강도 등을 동일하게 함으로써 다른 시술자간의 재현성을 높일 수 있다는 것을 확인했다. 추후 침실험에 있어서 여러 변수들에 의한 차이를 극복하고 재현성을 높일 수 있는 방법에 관한 더욱 심도 있는 연구가 필요하다.

키워드

참고문헌

  1. Bin Yan, Ke Li, Jianyang Xu, Wei Wang, Kuncheng Li, Hua liu, Baoci Shan, Xiaowei Tang. Acupoint-specific fMRI patterns in human brain. Neuroscience Letters. 2005 383 : 236-40. https://doi.org/10.1016/j.neulet.2005.04.021
  2. Fang JL, Krings T, Weidemann J, Meister IG, Thron A. Functional MRI in healthy subjects during acupuncture: different effects of needle rotation in real and false acupoints. Neuroradiology. 2004 46(5) : 359-62. https://doi.org/10.1007/s00234-003-1125-7
  3. Gareus, M. Lacour, A.C. Schulte, J. Hennig, Is there a BOLD response of the visual cortex on stimulation of the vision-related acupoint GB37? J. Magn. Reson. Imaging. 2002 15 : 227-32. https://doi.org/10.1002/jmri.10059
  4. Gusnard DA, Raichle ME. Searching for a baseline : functional imaging and the resting human brain. Nat Rev Neurosci. 2001 ; 2 : 685-94. https://doi.org/10.1038/35094500
  5. Hui KK, Liu J, Marina O, Napadow V, Haselqrove C, Kwong KK, Kennedy DN, Makris N. The integrated response of the human cerebro-cerebellar and limbic systems to acupuncture stimulation at ST36 as evidenced by fMRI. Neuroimage. 2005 27(3) : 479-96. https://doi.org/10.1016/j.neuroimage.2005.04.037
  6. Hui KKS, Liu J, Makris N, Gollub RL, Chen AJW, Moore CI, Kennedy DN, Rosen BR, Kwong KK. Acupuncture modilates the limbic system and subcortical gray structures of the human brain : evidence from fMRI studies in normal subjects. Hum Brain Mapp. 2000 9(1) : 13-25. https://doi.org/10.1002/(SICI)1097-0193(2000)9:1<13::AID-HBM2>3.0.CO;2-F
  7. Jeun SS, Kim JS, Kim BS, Park SD, Lim EC, Choi GS, Choe BY. Acupuncture stimulation for motor cortex activities: a 3T fMRI study. Am J Chin Med. 2005 33(4) : 573-8. https://doi.org/10.1142/S0192415X0500317X
  8. Jian Kong, Randy L Gollub, J. Megan Webb, Jiang-Ti Kong, Mark G Vangel, Kenneth Kwong, Test-retest study of fMRI signal change evoked by electroacupuncture stimulation. Neuroimage. 2007 February 1 34(3) : 1171–81.
  9. Kang (강)쇄빈, 경전침구학, 일중사, 서울, 2000, 223-5, 344-50.
  10. Kent A. Kiehl, Peter F. Liddle. Reproducibility of the hemodynamic Response to Auditory Oddball Stimuli: A Six-Week Test-Retest Study. Human Brain Mapping. 2003 18 : 42-52. https://doi.org/10.1002/hbm.10074
  11. Kong J, MA L, Gollub RL, Wei J, yang X, Li D, Weng X, Jia F, Wang C, Li F, Li R, Zhuang D. A pilot study of functional magnetic resonance imaging of the brain during manual and electroacupuncture stimulation of acupuncture point (LI-4 Hegu) in normal subjects reveals differential brain activation between methods. J Altern Complement Med. 2002 8(4) : 411-9. https://doi.org/10.1089/107555302760253603
  12. Li G, Cheung RT, Ma QY, Yang ES. Visual cortical activations on fMRI upon stimulation of the vision-implicated acupoints. Neuroreport. 2003 14(5) : 669-73. https://doi.org/10.1097/00001756-200304150-00002
  13. Li G, Huang L, Cheung RT, Liu SR, Ma QY, Yang ES. Cortical activations upon stimulation of the sensorimotorimplicated acupoints. Magn Reson Imaging. 2004 22(5) : 639-44. https://doi.org/10.1016/j.mri.2004.01.074
  14. Li G, Liu HL, Cheung RT, Hung YC, Wong KK, Shen GG, Ma QY, yang ES, An fMRI study comparing brain activation between word generation and electrical stimulation of languageimplicated acupoints. Hum Brain Mapp. 2003 : 18(3) : 233-8. https://doi.org/10.1002/hbm.10098
  15. Parrish TB, Schaeffer A, Catanese M, Rogel MJ. Functional magnetic resonance imaging of real and sham acupuncture. Noninvasively measuring cortical activation from acupuncture. IEEE Eng Med Biol Mag. 2005 24(2) : 35-40.
  16. Rombouts SARB, Barkhof F, Hoogenraad FGC, Sprenger M, Valk J, Scheltens P. Within-subject reproducibility of visual activation patterns with functional magnetic resonance imaging using multislice echo planar imaging. Magn Reson Imaging. 1998b 16 : 105-13. https://doi.org/10.1016/S0730-725X(97)00253-1
  17. Siedentopf CM, Golaszewski SM, MottaghyFM, Ruff CC, Felber S, Schlager A. Functional magnetic resonance imaging detects activation of the visual associaton cortex during laser acupuncture of the foot in humans. Neurosci Lett. 2002 327(1) : 53-6. https://doi.org/10.1016/S0304-3940(02)00383-X
  18. Stern CE, Corkin S, Gonzalez RG, Guimaraes AR, Baker JR, Jennings PJ, Carr CA, Sugiura RM, Vedantham V, Rosen BR. The hippocampal formation participates in novel picture encoding: Evidence from functional magnetic resonance imaging. Proc Natl Acad Sci USA. 1996 93 : 8660-5. https://doi.org/10.1073/pnas.93.16.8660
  19. Ueda Y, HayashiK, Kuriowa K. The application of fMRI to basic experiments in acupuncture. The effects of stimulus points and content on cerebral activities and responses. IEEE Eng Med Biol Mag. 2005 24(2) : 47-51.
  20. Wei-Ting Zhang, Zhen Jin, Fei Luo, Lei Zhang, Ya-Wei Zeng, Ji-Sheng Han. Evidence from brain imaging with fMRI supporting functional specificity of acupoints in humans. Neuroscience Letters. 2004 354 50-3. https://doi.org/10.1016/j.neulet.2003.09.080
  21. Willem CM Machielsen, Serge ARB Rombouts, Frederik Barkhof, Philip Scheltens, Menno P. Witter. fMRI of Visual Encoding: Reproducibility of Activation. Hum Brain Mapp. 2000 9 : 156-64. https://doi.org/10.1002/(SICI)1097-0193(200003)9:3<156::AID-HBM4>3.0.CO;2-Q
  22. Wu MT, Hiesh JC, Xiong J, Yang CF, Pan HB, Chen YC, Tsai G, Rosen BR, Kwong KK. Central nervous pathway for acupuncture stimulation : localization of processing with functional MR imaging of the brain - preliminary experience, Radiology. 1999 212(1) : 133-41.
  23. Wu MT, Sheen JM, Chuang KH, Yang P, Chin SL, Tasi CY, Chen CJ, Liao JR, Lai PH, Chu KA, Pan HB, Yang CF. Neuronal specificityof acupuncture response: a fMRI study with electroacupuncture. Neuroimage. 2002 16(4) : 1028-37. https://doi.org/10.1006/nimg.2002.1145
  24. Yan B, Li K, Xu J, Wang W, Li K, Liu H, Shan B, Tang X. Acupoint-specific fMRI patterns in human brain. Neurosci Lett. 2005 383(3) : 236-40. https://doi.org/10.1016/j.neulet.2005.04.021
  25. Yoo SS, Teh EK, Blinder RA, Jolesz FA. Modulation of cerebellar activities by acupuncture stimulation: evidence from fMRI study. Neuroimage. 2004 22(2) : 932-40. https://doi.org/10.1016/j.neuroimage.2004.02.017
  26. Zhang WT, Jin Z, Cui GH, Zhang KL, Zhang L, Zeng YW, Luo F, Chen AC, Han JS. Relations between brain network activation and analgesic effect induced by low vs. high frequency electrical acupoint stimulation in different subjects: a functional magnetic resonance imaging study. Brain Res. 2003 982(2) : 168-78. https://doi.org/10.1016/S0006-8993(03)02983-4
  27. Zhang WT, Jin Z, Luo F, Zhang L, Zeng YW, Han JS. Evidence from brain imaging with fMRI supporting functional specificity of acupoints in humans. Neurosci Lett. 2004 354(1) : 50-3. https://doi.org/10.1016/j.neulet.2003.09.080