Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
권호 표지

Effects of Electroacupuncture on the Visceromotor Response to Colonic Distension in TNBS-Induced Colonic Inflammation in Rats

흰쥐의 TNBS 유발 대장염에서 전침(電鍼)의 대장(大腸) 흥분성(興奮性)에 미치는 효과(效果)

  • Choi, Yun-Young (Dept. of Meridian & Acupoint, College of Korean Medicine, Wonkwang University) ;
  • Ahn, Sung-Hun (Dept. of Meridian & Acupoint, College of Korean Medicine, Wonkwang University) ;
  • Kwon, Oh-Sang (Dept. of Meridian & Acupoint, College of Korean Medicine, Wonkwang University) ;
  • Seo, Sang-Rok (Dept. of Meridian & Acupoint, College of Korean Medicine, Wonkwang University) ;
  • Sohn, In-Chul (Dept. of Meridian & Acupoint, College of Korean Medicine, Wonkwang University) ;
  • Kim, Jae-Hyo (Dept. of Meridian & Acupoint, College of Korean Medicine, Wonkwang University)
  • 최윤영 (원광대학교 한의과대학 경락경혈학교실) ;
  • 안성훈 (원광대학교 한의과대학 경락경혈학교실) ;
  • 권오상 (원광대학교 한의과대학 경락경혈학교실) ;
  • 서상록 (원광대학교 한의과대학 경락경혈학교실) ;
  • 손인철 (원광대학교 한의과대학 경락경혈학교실) ;
  • 김재효 (원광대학교 한의과대학 경락경혈학교실)
  • Published : 2009.06.27
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Abstract

Objectives: Single colorectal instillation of trinitrobenzenesulphonic acid (TNBS) causes a dose-dependent increase of visceral motor response (VMR) and severity of inflammation. In this study we compared the effects of electroacupuncture in the different acupoints in the acute colitis induced by TNBS intracolonic injection in rats. Methods: In Male Sprague-Dawley rats, weighing $250{\sim}400g$, a single colorectal administration of TNBS 5mg/kg and 50% ethanol under isoflurane anaesthesia after an overnight fast. Electrodes for electromyography (EMG) recording were stitched into the external oblique musculature under general anesthesia. Acupoints of LI4, ST25, or ST36 were stimulated by electroacupuncture, respectively. The balloon was inserted intra-anally and visceral motor response (VMR) to colorectal distensioin (CRD) was quantified with an EMG recording system. Results: At an observation of the visceral hyperalgesia in the day-time series, the visceromotor response increased significantly 3 days after TNBS intra-rectalcolonic injection in rats. Electroacupuncture on either ST25 or ST36 suppressed the visceromotor response to colorectal distension, but not LI4, at 3 days after TNBS injection. Pretreatment of naltrexone (10 mg/kg, i.p.), opioids antagonist, inhibited the VMR suppress of 10Hz EA to ST36 but not phentolamine (5 mg/kg, i.p.). Pretreatment of either naltrexone or phentolamine inhibited effects of 10Hz EA to ST25. Conclusions: Data show that EA at either ST25 or ST36 potently inhibits hypersensitivity of colorectum after TNBS induced colitis and is differently mediated through the endogenous opioid system and adrenergic system.

Keywords

References

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