Parasites, Hosts and Diseases

  • 제47권3호
  • /
  • Pages.219-225
  • /
  • 2009
  • /
  • 2982-5164(pISSN)
  • /
  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
권호 표지
DOI QR코드

DOI QR Code

A Hospital-Based Serological Survey of Cryptosporidiosis in the Republic of Korea

  • Lee, Jong-Kyu (Department of Environmental and Tropical Medicine, Konkuk University School of Medicine) ;
  • Han, Eun-Taek (Department of Parasitology, Kangwon National University College of Medicine) ;
  • Huh, Sun (Department of Parasitology, College of Medicine, Hallym University) ;
  • Park, Woo-Yoon (Department of Radiation Oncology, College of Medicine, Chungbuk National University) ;
  • Yu, Jae-Ran (Department of Environmental and Tropical Medicine, Konkuk University School of Medicine)
  • 발행 : 2009.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The seroprevalence of cryptosporidiosis was examined using patients' sera collected from hospitals located in 4 different areas of the Republic of Korea. ELISA was used to measure antibody titers against Cryptosporidium parvum antigens from a total of 2,394 serum samples, which were collected randomly from patients in local hospitals; 1)Chungbuk National University Hospital, 2) Konkuk University Hospital, 3) local hospitals in Chuncheon, Gangwon-do (province), 4) Jeonnam National University Hospital, from 2002 through 2003. Of the2,394 samples assayed, 34%, 26%, and 56% were positive for C. parvum-specific IgG, IgM, and IgA antibodies, respectively, Positive IgG titers were most common in sera from Jeonnam National University Hospital, Gwangju, Jeollanam-do, and positive IgM titers were most common in sera from Chungbuk National University Hospital, Cheongju, Chuncheonqbuk-do. The seropositivity was positively correlated with age for both the IgG and IgA antibodies but was negatively correlated with age for the IgM antibodies. Western blotting revealed that 92%, 83%, and 77% of sera positive for IgG, IgM, and IgA ELISA reacted with 27-kDa antigens, respectively. These results suggested that infection with Cryptosporidium in hospital patients occurs more commonly than previously reported in the Republic of Korea.

키워드

참고문헌

  1. Fayer R. Cryptosporidium and cryptosporidiosis. Boca Raton, Florida, USA. CRC Press. 1997, p 1-251
  2. DuPont HL, Chappell CL, Sterling CR, Okhuysen PC, Rose JB, Jakubowski W. The infectivity of Cryptosporidium parvum in healthy volunteers. N Engl J Med 1995; 332: 855-859 https://doi.org/10.1056/NEJM199503303321304
  3. Pozio E, Rezza G, Boschini A, Pezzotti P, Tamburrini A, Rossi P, Di Fine M, Smacchia C, Schiesari A, Gattei E, Zucconi R, Ballarini P. Clinical cryptosporidiosis and human immunodeficiency virus (HIV)-induced immunosuppression: findings from a longitudinal study of HIV-positive and HIV-negative former injection drug users. J Infect Dis 1997; 176: 969-975 https://doi.org/10.1086/516498
  4. Ramirez NE, Ward LA, Sreevatsan S. A review of the biology and epidemiology of cryptosporidiosis in humans and animals. Microbes Infect 2004; 6: 773-785 https://doi.org/10.1016/j.micinf.2004.02.021
  5. Current WL, Garcia LS. Cryptosporidiosis. Clin Microbiol Rev 1991; 4: 325-358
  6. Cho MH, Kim AK, Im KI. Detection of Cryptosporidium oocysts from out-patients of the Severance Hospital, Korea. Korean J Parasitol 1993; 31: 193-199 https://doi.org/10.3347/kjp.1993.31.3.193
  7. Chai JY, Lee SH, Guk SM, Lee SH. An epidemiological survey of Cryptosporidium parvum infection in randomly selected inhabitants of Seoul and Chollanam-do. Korean J Parasitol 1996; 34: 113-119 https://doi.org/10.3347/kjp.1996.34.2.113
  8. Seo M, Huh S, Chai JY, Yu JR. An epidemiological survey on Cryptosporidium parvum infection of inhabitants in Chorwon-gun, Kangwon-do. Korean J Parasitol 2001; 39: 201-203 https://doi.org/10.3347/kjp.2001.39.2.201
  9. Lee JK, Song HJ, Yu JR. Prevalence of diarrhea caused by Cryptosporidium parvum in non-HIV patients in Jeollanam-do, Korea. Korean J Parasitol 2005; 43: 111-114 https://doi.org/10.3347/kjp.2005.43.3.111
  10. Guk SM, Seo M, Park YK, Oh MD, Choe KW, Kim JL, Choi MH, Hong ST, Chai JY. Parasitic infections in HIV-infected patients who visited Seoul National University Hospital during the period 1995-2003. Korean J Parasitol 2005; 43: 1-5 https://doi.org/10.3347/kjp.2005.43.1.1
  11. Weber R, Bryan RT, Bishop HS, Wahlquist SP, Sullivan JJ, Juranek DD. Threshold of detection of Cryptosporidium oocysts in human stool specimens: evidence for low sensitivity of current diagnostic methods. J Clin Microbiol 1991; 29: 1323-1327
  12. Kuhls TL, Mosier DA, Crawford DL, Griffis J. Seroprevalence of cryptosporidial antibodies during infancy, childhood, and adolescence. Clin Infect Dis 1994; 18: 731-735 https://doi.org/10.1093/clinids/18.5.731
  13. Zu SX, Li JF, Barrett LJ, Fayer R, Shu SY, McAuliffe JF, Roche JK, Guerrant RL. Seroepidemiologic study of Cryptosporidium infection in children from rural communities of Anhui, China and Fortaleza, Brazil. Am J Trop Med Hyg 1994; 51: 1-10 https://doi.org/10.4269/ajtmh.1994.51.1
  14. Moss DM, Chappell CL, Okhuysen PC, DuPont HL, Arrowood MJ, Hightower AW, Lammie PJ. The antibody response to 27-, 17-, and 15-kDa Cryptosporidium antigens following experimental infection in humans. J Infect Dis 1998; 178: 827-833 https://doi.org/10.1086/515377
  15. McDonald AC, Mac Kenzie WR, Addiss DG, Gradus MS, Linke G, Zembrowski E, Hurd MR, Arrowood MJ, Lammie PJ, Priest JW. Cryptosporidium parvum-specific antibody responses among children residing in Milwaukee during the 1993 waterborne outbreak. J Infect Dis 2001; 183: 1373-1379 https://doi.org/10.1086/319862
  16. Priest JW, Xie LT, Arrowood MJ, Lammie PJ. The immunodominant 17-kDa antigen from Cryptosporidium parvum is glycosylphosphatidylinositol-anchored. Mol Biochem Parasitol 2001; 113: 117-126 https://doi.org/10.1016/S0166-6851(00)00386-8
  17. Khan WA, Rogers KA, Karim MM, Ahmed S, Hibberd PL, Calderwood SB, Ryan ET, Ward HD. Cryptosporidiosis among Bangladeshi children with diarrhea: a prospective, matched, casecontrol study of clinical features, epidemiology and systemic antibody responses. Am J Trop Med Hyg 2004; 71: 412-419
  18. Ong CS, Li AS, Priest JW, Copes R, Khan M, Fyfe MW, Marion SA, Roberts JM, Lammie PJ, Isaac-Renton JL. Enzyme immunoassay of Cryptosporidium-specific immunoglobulin G antibodies to assess longitudinal infection trends in six communities in British Columbia, Canada. Am J Trop Med Hyg 2005; 73: 288-295
  19. Teixeira MC, Barreto ML, Melo C, Silva LR, Moraes LR, Alcantara-Neves NM. A serological study of Cryptosporidium transmission in a periurban area of a Brazilian Northeastern city. Trop Med Int Health 2007; 12: 1096-1104 https://doi.org/10.1111/j.1365-3156.2007.01892.x
  20. Frost FJ, de la Cruz AA, Moss DM, Curry M, Calderon RL. Comparisons of ELISA and western blot assays for detection of Cryptosporidium antibody. Epidemiol Infect 1998; 121: 205-211 https://doi.org/10.1017/S0950268898008991
  21. Priest JW, Kwon JP, Moss DM, Roberts JM, Arrowood MJ, Dworkin MS, Juranek DD, Lammie PJ. Detection by enzyme immunoassay of serum immunoglobulin G antibodies that recognize specific Cryptosporidium parvum antigens. J Clin Microbiol 1999; 37: 1385-1392
  22. O'Donoghue PJ. Cryptosporidium and cryptosporidiosis in man and animals. Int J Parasitol 1995; 25: 139-195 https://doi.org/10.1016/0020-7519(94)E0059-V
  23. Yang S, Healey MC. The immunosuppressive effects of dexamethasone administered in drinking water to C57BL/6N mice infected with Cryptosporidium parvum. J Parasitol 1993; 79: 626-630 https://doi.org/10.2307/3283395
  24. Waldman E, Tzipori S, Forsyth JR. Separation of Cryptosporidium species oocysts from feces by using a percoll discontinuous density gradient. J Clin Microbiol 1986; 23: 199-200
  25. Leach CT, Koo FC, Kuhls TL, Hilsenbeck SG, Jenson HB. Prevalence of Cryptosporidium parvum infection in children along the Texas-Mexico border and associated risk factors. Am J Trop Med Hyg 2000; 62: 656-661
  26. Ungar BL, Gilman RH, Lanata CF, Perez-Schael I. Seroepidemiology of Cryptosporidium infection in two Latin American populations. J Infect Dis 1988; 157: 551-556 https://doi.org/10.1093/infdis/157.3.551
  27. Frost FJ, Fea E, Gilli G, Biorci F, Muller TM, Craun GF, Calderon RL. Serological evidence of Cryptosporidium infections in southern Europe. Eur J Epidemiol 2000; 16: 385-390 https://doi.org/10.1023/A:1007654226975
  28. Yu JR, Lee JK, Seo M, Kim SI, Sohn WM, Huh S, Choi HY, Kim TS. Prevalence of cryptosporidiosis among the villagers and domestic animals in several rural areas of Korea. Korean J Parasitol 2004; 42: 1-6 https://doi.org/10.3347/kjp.2004.42.1.1
  29. Park JH, Kim HJ, Guk SM, Shin EH, Kim JL, Rim HJ, Lee SH, Chai JY. A survey of cryptosporidiosis among 2,541 residents of 25 coastal islands in Jeollanam-do (Province), Republic of Korea. Korean J Parasitol 2006; 44: 367-372 https://doi.org/10.3347/kjp.2006.44.4.367
  30. Kosek M, Alcantara C, Lima AA, Guerrant RL. Cryptosporidiosis: an update. Lancet Infect Dis 2001; 1: 262-269 https://doi.org/10.1016/S1473-3099(01)00121-9
  31. Okhuysen PC, Chappell CL, Sterling CR, Jakubowski W, DuPont HL. Susceptibility and serologic response of healthy adults to reinfection with Cryptosporidium parvum. Infect Immun 1998; 66: 441-443
  32. Mead JR, Arrowood MJ, Sterling CR. Antigens of Cryptosporidium sporozoites recognized by immune sera of infected animals and humans. J Parasitol 1988; 74: 135-143 https://doi.org/10.2307/3282489
  33. Moss DM, Bennett SN, Arrowood MJ, Wahlquist SP, Lammie PJ. Enzyme-linked immunoelectrotransfer blot analysis of a cryptosporidiosis outbreak on a United States Coast Guard cutter. Am J Trop Med Hyg 1998; 58: 110-118

피인용 문헌

  1. Characterization of the thioredoxin peroxidase from Cryptosporidium parvum vol.129, pp.4, 2009, https://doi.org/10.1016/j.exppara.2011.09.011
  2. Clinical Guidelines for the Treatment and Prevention of Opportunistic Infections in HIV-infected Koreans vol.44, pp.3, 2009, https://doi.org/10.3947/ic.2012.44.3.93
  3. A Waterborne Outbreak and Detection of Cryptosporidium Oocysts in Drinking Water of an Older High-Rise Apartment Complex in Seoul vol.51, pp.4, 2009, https://doi.org/10.3347/kjp.2013.51.4.1