Parasites, Hosts and Diseases

  • 제47권3호
  • /
  • Pages.287-291
  • /
  • 2009
  • /
  • 2982-5164(pISSN)
  • /
  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
권호 표지
DOI QR코드

DOI QR Code

Evaluation of ${\alpha}$-Tubulin as an Antigenic and Molecular Probe to Detect Giardia lamblia

  • Kim, Ju-Ri (Department of Environmental Medical Biology and Institute of Tropical Medicine, Post Brain Korea 21 Program, Yonsei University College of Medicine) ;
  • Shin, Myeong-Heon (Department of Environmental Medical Biology and Institute of Tropical Medicine, Post Brain Korea 21 Program, Yonsei University College of Medicine) ;
  • Song, Kyoung-Ju (Department of Environmental Medical Biology and Institute of Tropical Medicine, Post Brain Korea 21 Program, Yonsei University College of Medicine) ;
  • Park, Soon-Jung (Department of Environmental Medical Biology and Institute of Tropical Medicine, Post Brain Korea 21 Program, Yonsei University College of Medicine)
  • 발행 : 2009.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The ${\alpha}/{\beta}$-tubulin heterodimer is the basic subunit of microtubules in eukaryotes. Polyclonal antibodies specific to recombinant ${\alpha}$-tubulin of Giardia lamblia were made, and found effective as a probe to specifically detect G. lamblia by immunofluorescence assays. Nucleotide sequences of ${\alpha}$-tubulin genes were compared between G. lamblia WB and GS strains, prototypes of assemblage A and assemblage B, respectively. A set of primers was designed and used to amplify a portion of the ${\alpha}$-tubulin gene from G. lamblia. PCR-RFLP analysis of this ${\alpha}$-tubulin PCR product successfully differentiated G. lamblia into 2 distinct groups, assemblages A and B.Theresults indicate that ${\alpha}$-tubulin can be used as a molecular probe to detect G.lamblia.

키워드

참고문헌

  1. Adam RD. The biology of Giardia spp. Microbiol Rev 1991; 55: 706-732
  2. Mayrhoefer G, Andrews RH, Ey PL, Chilton NB. Division of Giardia isolates from humans into two genetically distinct assemblages by electrophoretic analysis of enzymes encoded at 27 loci and comparison with Giardia muris. Parasitology 1995; 111: 11-17 https://doi.org/10.1017/S0031182000064556
  3. Andrews RH, Adams M, Boreham PF, Mayrhoefer G, Meloni BP. Giardia intestinalis: electrophoretic evidence for species complex. Int J Parasitol 1989; 19: 183-190 https://doi.org/10.1016/0020-7519(89)90006-4
  4. Monis PT, Mayrhoefer G, Andrews RH, Homan WL, Limper L, Ey PL. Molecular genetic analysis of Giardia intestinalis isolates at the glutamate dehydrogenase locus. Parasitology 1996; 11: 1-12 https://doi.org/10.1017/S0031182000004042
  5. Homan WL, Van Enckevort FH, Limper L, Van Eys GJ, Schoone GJ, Kasprzak W, Majewska AC, Van Knapen F. Comparison of Giardia isolates from different laboratories by isoenzyme analysis and recombinant DNA probes. Parasitol Res 1992; 78: 316-323 https://doi.org/10.1007/BF00937090
  6. Aggarwal A, Nash TE. Comparison of two antigenically distinct Giardia lamblia isolates in gerbils. Am J Trop Med Hyg 1987; 36: 325-332 https://doi.org/10.4269/ajtmh.1987.36.325
  7. Byrd LG, Conrad JT, Nash TE. Giardia lamblia infections in adult mice. Infect Immun 1994; 62: 3583-3585
  8. Monis PT, Andrews RH, Mayrhoefer G, Mackrill J, Kulda J, Isaac-Renton JL, Ey PL. Novel lineages of Giardia intestinalis identified by genetic analysis of organisms isolated from dogs in Australia. Parasitology 1998; 116: 7-19 https://doi.org/10.1017/S0031182097002011
  9. Ey PL, Mansouri M, Kulda J, Nohynkova E, Monis PT, Andrews RH, Mayrhoefer G. Genetic analysis of Giardia from hoofed farm animals reveals artiodactyls-specific and potentially zoonotic genotypes. J Eukaryot Microbiol 1997; 44: 626-635 https://doi.org/10.1111/j.1550-7408.1997.tb05970.x
  10. Monis PT, Andrews RH, Mayrhoefer G, Ey PL. Molecular systematics of the parasitic protozoan Giardia intestinalis. Mol Biol Evol 1999; 16: 1135-1144 https://doi.org/10.1093/oxfordjournals.molbev.a026204
  11. Weiss JB, Van Keulen H, Nash TE. Classification of subgroups of Giardia lamblia based upon ribosomal RNA gene sequence using polymerase chain reaction. Mol Biochem Parasitol 1992; 54: 73-86 https://doi.org/10.1016/0166-6851(92)90096-3
  12. Hopkins RM, Meloni BP, Groth DM, Wetherall JD, Reynoldson JA, Thompson RC. Ribosomal RNA sequencing reveals differences between the genotypes of Giardia isolates recovered from humans and animals in Germany. J Parasitol 1997; 83: 44-51 https://doi.org/10.2307/3284315
  13. Baruch AC, Isaac-Renton J, Adam RD. The molecular epidemiology of Giardia lamblia: a sequence-based approach. J Infect Dis 1996; 174: 233-236 https://doi.org/10.1093/infdis/174.1.233
  14. Mahbubani MH, Bej AK, Perlin M, Schafer III FW, Jakubowski W, Atlas RM. Differentiation of Giardia duodenalis from other Giardia spp. by using polymerase chain reaction and gene probes. J Clin Microbiol 1992; 30: 74-78
  15. Yong TS, Park SJ, Hwang UW, Yang HW, Lee K, Min DY, Rim HJ, Wang Y, Feng Z. Ribosomal DNA sequencing for genotyping of Giardia lamblia recovered from humans in China and Korea. J Parasitol 2000; 86: 887-891 https://doi.org/10.2307/3284991
  16. Keister DB. Axenic culture of Giardia lamblia in TYI-S-33 medium supplemented with bile. Trans R Soc Trop Med Hyg 1983; 77: 487-488 https://doi.org/10.1016/0035-9203(83)90120-7
  17. Diamond LS. The establishment of various trichomonads of animals and man in axenic cultures. J Parasitol 1957; 43: 488-490
  18. Diamond LS. Axenic cultivation of Entamoeba histolytica. Science 1961; 134: 336-337 https://doi.org/10.1126/science.134.3475.336
  19. Willaert E. Isoelement et culture in vitro des amibes genre Naegleria. Ann Soc Belge Med Trop 1971; 51: 701-708
  20. Park SJ, Yong TS, Yang HW, Lee DH, Lee K. Axenic cultivation and characterization of Giardia lamblia isolated from humans in Korea. Korean J Parasitol 1999; 37: 121-125 https://doi.org/10.3347/kjp.1999.37.2.121
  21. Zhang Q, Zhu L, Feng H, Ang S, Chau FS, Liu WT. Microbial detection in microfluidic devices through dual staining of quantum dots-labeled immunoassay and RNA hybridization. Anal Chum Acta 2006; 556: 171-177 https://doi.org/10.1016/j.aca.2005.07.003
  22. Bertrand I, Gantzer C, Chesnot T, Schwartzbrod J. Improved specificity for Giardia lamblia cysts quantification in wastewater by development of a real-time PCR method. J Microb Methods 2004; 57: 41-53 https://doi.org/10.1016/j.mimet.2003.11.016
  23. Souza DS, Barreiros JT, Papp KM, Steindel M, Simoes CM, Barardi CR. Comparison between immunomagnetic separation, coupled with immunofluorescence, and the techniques of Faust et al. and of Lutz for diagnosis of Giardia lamblia cysts in human feces. Rev Inst Med Trop Sao Paulo 2003; 45: 339-342 https://doi.org/10.1590/S0036-46652003000600008
  24. Ratanapo S, Mungthin M, Soontrapa S, Faithed C, Siripattanapipong S, Rangsin R, Naaglor T, Piyaraj P, Taamasri P, Leelayoova S. Multiple modes of transmission of giardiasis in primary schoolchildren of a rural community, Thailand. Am J Trop Med Hyg 2008; 78: 611-615
  25. Robertson LJ, Forberg T, Hermansen L, Hamnes IS, Gjerde B. Giardia duodenalis cysts isolated from wild moose and reindeer in Norway: genetic characterization of PCR-RFLP and sequence analysis at two genes. J Wild Dis 2007; 43: 576-585 https://doi.org/10.7589/0090-3558-43.4.576

피인용 문헌

  1. Identification of genotypes of Giardia duodenalis human isolates in Isfahan, Iran, using polymerase chain reaction – Restriction Fragment Length polymorphism vol.1, pp.None, 2009, https://doi.org/10.4103/2277-9175.105166
  2. Host defences against Giardia lamblia vol.37, pp.8, 2015, https://doi.org/10.1111/pim.12210